Senior Scientist, Cytometric Profiling

Merck; South San Francisco (CA); On-site; USD 131,000 - 207,000; Full time

Benefits offered by this job

Annual bonus

Long-term incentive

Benefits package

Job summary

Merck in South San Francisco is seeking an experienced scientist to join the Profiling & Expression team. You will design and execute deep profiling of primary human tissue using flow cytometry, develop biomarker assays, and discuss results with stakeholders across R&D to support translational programs.

The role emphasizes strong cytometry and immunology skills, cell culture, and collaboration in a fast-paced environment with opportunities for growth.

Qualifications

• Experience with traditional Flow Cytometry (BD/Beckman).
• Knowledge of Spectral Cytometry (Cytek).
• Proficiency with FlowJo data analysis software.
• Experience in immunologic assays and cell culture techniques.

Responsibilities

• Independently designing and executing properly controlled experiments for deep profiling of primary human tissue using flow cytometry in a BSL-2 laboratory environment.
• Developing flow cytometry-based preclinical biomarker assays.
• Representing the Cytometric Profiling group in team meetings with cross-functional stakeholders.
• Presenting results at meetings and maintaining detailed notebooks.
• Support daily operations of FACS core lab.

Skills

Flow Cytometry

Immunology

Cell Culture

Biomarker Assays

Communication

Education

Bachelor's degree (Biology)

Master's degree

PhD in Biology/Immunology

Tools

BD Biosciences

Beckman Coulter

Cytek

FlowJo

Job description

Job Description

The Profiling & Expression department within Translational Medicine helps bridge Discovery and Development programs in multiple therapeutic areas through expertise in multimodal molecular analysis of tissues and model systems. We are a platform-based functional group leveraging a close partnership of pathologists, gene expression scientists, image analysis specialists, assay developers, and data scientists to contribute to target validation, generation of quality mechanistic data, and the discovery and development of response and pharmacodynamic biomarkers with line of sight to the clinic. The Cytometric Profiling group within the Profiling & Expression department leverages high dimensional flow cytometry for target expression analysis, target engagement assay development, and pharmacodynamic biomarker discovery predominantly using primary human tissues. The successful candidate will focus on the Immunology therapy area by profiling therapeutic targets of interest in primary human healthy and disease tissue using high-dimensional flow cytometry. Indications relevant to this work include autoimmune, inflammatory, atopic, and fibrotic diseases. Profiling data will support preclinical and early clinical program development as well as translational efforts in Immunology or other therapy areas as needed. This position requires a proactive, innovative, organized, and detail-oriented scientist who can work collaboratively across multiple functional areas to support the development of our company's pipeline.

Job Responsibilities Include The Following

• Independently designing and executing properly controlled experiments for deep profiling of primary human tissue using flow cytometry in a BSL-2 laboratory environment
• Developing flow cytometry-based preclinical biomarker assays
• Representing the Cytometric Profiling group in team meetings while actively engaging with stakeholders across the Research and Development Division's network
• Presenting results at lab, team, or department meetings
• Maintaining a detailed, complete, and compliant laboratory notebook
• Help supporting daily operations of FACS core lab

Qualifications

Education

• Bachelor's (with 7+ years), Master's (with 4+ years) or Ph.D. in Biology, Immunology, or related discipline and relevant industry or postdoctoral experience

Required Experience And Skills

• Experience with traditional Flow Cytometry, preferably with BD Biosciences or Beckman Coulter flow cytometers
• Knowledge of Spectral Cytometry, preferably with Cytek Biosciences spectral cytometers
• Proficiency with FlowJo data analysis software
• Knowledge of immunologic assays: T cell and myeloid cell stimulation, phospho-STAT or NFkB intracellular staining, ELISA
• Cell culture and tissue dissociation techniques
• Excellent communication and interpersonal skills, as well as the ability to function in a team environment
• Ability to interface with translational, discovery, and clinical cross-functional colleagues to drive assay development and implementation into discovery and/or clinical programs as needed
• Proficiency with MS Office applications on the Windows operating system

Preferred Experience And Skills

• Immunology and/or Immuno-Oncology background
• Experience with cell sorting (FACS), preferably with Cytek spectral cell sorters
• Cell preparation for single cell RNA-sequencing as well as Total-seq staining
• Single cell RNA-sequencing using the 10X Genomics platform
• Proficiency with high-dimensional data clustering and analytical methods
• Proficiency with TIBCO Spotfire, GraphPad Prism, OMIQ
• With management guidance, ability to incorporate cutting edge scientific and technical advances to support Profiling & Expression priorities

Required Skills

Cell Culture Techniques, Cytometry, Flow Cytometry, Immunoassays, Immunology, Immunology Research, T Cell Immunology, Tissue Cultures

US And Puerto Rico Residents Only

Our company is committed to inclusion, ensuring that candidates can engage in a hiring process that exhibits their true capabilities.

Requirements

As an Equal Employment Opportunity Employer, we provide equal opportunities to all employees and applicants for employment and prohibit discrimination on the basis of race, color, age, religion, sex, sexual orientation, gender identity, national origin, protected veteran status, disability status, or other applicable legally protected characteristics. As a federal contractor, we comply with all affirmative action requirements for protected veterans and individuals with disabilities. For more information about personal rights under the U.S. Equal Opportunity Employment laws, visit: EEOC Know Your Rights EEOC GINA Supplement We are proud to be a company that embraces the value of bringing together, talented, and committed people with diverse experiences, perspectives, skills and backgrounds. The fastest way to breakthrough innovation is when people with diverse ideas, broad experiences, backgrounds, and skills come together in an inclusive environment. We encourage our colleagues to respectfully challenge one another’s thinking and approach problems collectively. Learn more about your rights, including under California, Colorado and other US State Acts.

San Francisco Residents Only:

We will consider qualified applicants with arrest and conviction records for employment in compliance with the San Francisco Fair Chance Ordinance

Los Angeles Residents Only:

We will consider for employment all qualified applicants, including those with criminal histories, in a manner consistent with the requirements of applicable state and local laws, including the City of Los Angeles’ Fair Chance Initiative for Hiring Ordinance

Employee Status Regular

Relocation Domestic/International

VISA Sponsorship Yes

Travel Requirements No Travel Required

Flexible Work Arrangements Not Applicable

Shift 1st - Day

Valid Driving License No

Hazardous Material(s) N/A

Job Posting End Date

• A job posting is effective until 11:59:59PM on the day BEFORE the listed job posting end date. Please ensure you apply to a job posting no later than the day BEFORE the job posting end date.

Requisition ID: R416348

The salary range for this role is $131,400.00 - $206,800.00 This is the lowest to highest salary we in good faith believe we would pay for this role at the time of this posting. An employee’s position within the salary range will be based on several factors including, but not limited to relevant education, qualifications, certifications, experience, skills, geographic location, government requirements, and business or organizational needs.

The successful candidate will be eligible for annual bonus and long-term incentive, if applicable.

We offer a comprehensive package of benefits. Available benefits include medical, dental, vision healthcare and other insurance benefits (for employee and family), retirement benefits, including 401(k), paid holidays, vacation, and compassionate and sick days. More information about benefits is available at https://jobs.merck.com/us/en/compensation-and-benefits.