Senior Research Assistant, Leukemia Translational Lab Lead

Cincinnati Children's Hospital Medical Center; Cincinnati (OH); In loco; USD 53.000 - 72.000; Tempo pieno

Descrizione del lavoro

Cincinnati Children's Hospital Medical Center in Cincinnati, OH is seeking an experienced Research Assistant to serve as a senior research professional and laboratory manager in a translational leukemia lab focused on understanding and overcoming targeted therapy resistance in AML.

You will support scientific and operational aspects of the lab, including experiments, regulatory compliance, trainee support, and inventory management.

Competenze

• Bachelor's degree in a related field with 1 year of directly related experience or Master's degree in a related field.
• Preferred experience with mammalian tissue culture, DNA/RNA isolation, protein biochemistry, sequencing workflows, and in vivo planning.
• Strong organizational, record-keeping, and project management skills.
• Ability to work independently and collaboratively in a fast-paced research environment.
• Experience in leukemia or hematologic malignancies and handling primary patient-derived samples is a plus.

Mansioni

• Perform and troubleshoot molecular and cellular biology experiments with minimal supervision.
• Maintain and characterize leukemia cell lines and primary patient samples.
• Conduct tissue culture studies, in vitro drug treatment experiments, and mechanistic studies of therapeutic response and resistance.
• Perform protein-based analyses, including immunoblotting, immunoprecipitation (IP), coimmunoprecipitation (co-IP), and related assays.
• Isolate DNA and RNA from cells, tissues, and animal samples.
• Prepare samples for next-generation sequencing and assist with sequencing-based studies.
• Support in vivo studies, including planning, coordination, sample collection, and analysis of mouse experiments.
• Maintain accurate, detailed, and well-organized experimental records and laboratory notebooks.
• Analyze, summarize, and present experimental findings at laboratory meetings.
• Oversee day-to-day laboratory operations and ensure efficient workflow.
• Manage laboratory inventory, purchasing, equipment maintenance, and vendor interactions.
• Develop and maintain laboratory SOPs and organizational systems.
• Coordinate laboratory onboarding and training of students, staff, and trainees.
• Monitor compliance with institutional safety, biosafety, animal use, and regulatory requirements.
• Assist with laboratory resource allocation.
• Foster a collaborative and productive laboratory environment.

Conoscenze

Mammalian tissue culture

DNA/RNA isolation

Protein biochemistry

Immunoblotting

Immunoprecipitation

Molecular biology

Sequencing workflows

Mouse research

Laboratory management

Formazione

Bachelor's degree in related field

Master's degree in related field

1 year related experience

3 years related experience

Master's degree with 2 years related experience

Strumenti

Next-generation sequencing

Workflow management

Regulatory compliance

Descrizione del lavoro

Cincinnati Children's Hospital Medical Center in Cincinnati, OH is seeking an experienced Research Assistant to serve as a senior research professional and laboratory manager in a translational leukemia lab focused on understanding and overcoming targeted therapy resistance in AML.

You will support scientific and operational aspects of the lab, including experiments, regulatory compliance, trainee support, and inventory management.