Scientist, Proteomics-----Cambridge, MA

Zenith LifeScience LLC.; Cambridge (MA); On-site; USD 120,000 - 160,000; Full time

Job summary

Zenith LifeScience LLC is seeking a highly motivated scientist to join the Chemical Biology and Proteomics group within Discovery Sciences. You will apply proteomics to advance drug discovery and interrogate mechanisms of action in CVRM contexts.

You will design mass-spectrometry–based discovery assays, perform proximity biotinylation, and validate protein interactions with PLA and cell-based assays while maintaining rigorous notebooks and collaborating across disciplines.

Qualifications

• PhD with at least 2 years hands-on proteomics experience, or Masters with 3–5 years proteomics experience.
• Experience with proteomic sample preparation.
• Experience in mammalian cell culture.
• Ability to work independently and in cross-functional teams.

Responsibilities

• Design and execute discovery proteomic assays using mass spectrometry.
• Perform proximity biotinylation studies in cells with automated affinity purification and DIA MS methods.
• Develop proximity ligation assays and cell-based assays to assess protein interactions.
• Generate protein conjugates and characterize labeling by UV-Vis spectroscopy.
• Analyze and interpret data using established bioinformatic pipelines.
• Maintain detailed and accurate electronic lab notebooks.
• Represent CBP team and communicate with project discipline leads to generate mechanistic hypotheses from proteomics data.

Skills

Proteomics

Mass spectrometry

Proximity ligation assays

Mammalian cell culture

Data analysis pipelines

Team collaboration

Education

Ph.D. in Proteomics / Chemical Biology / Biological Sciences / Biochemistry or related

Masters with 3–5 years hands-on proteomics experience

Tools

Spectronaut

Proteome Discoverer

MaxQuant

Skyline

Xcalibur

Job description

We are seeking a highly motivated and talented scientist to join Company’s Chemical Biology and Proteomics group within Discovery Sciences. You will apply proteomics to advance drug discovery. You will work as an integral member of a cross-functional team to interrogate mechanisms of action for therapeutic agents, and to propose and validate biological hypotheses for agents within the cardiovascular, renal and metabolism (CVRM) area.

Key Responsibilities:

• Design and execute discovery proteomic assays using mass spectrometry to support mechanistic studies, Client Client targets, and elucidate biological regulators of disease biology.
• Perform proximity biotinylation studies in cells, using established automated affinity purification workflows, and data independent acquisition (DIA) mass spectrometry methods.
• Perform target validation by developing proximity ligation assays (PLA) and cell-based assays to assess protein-protein interactions using chemical tools and confocal microscopy.
• Generate protein conjugates and characterize protein labeling by UV-Vis spectroscopy.
• Analyze and interpret experimental data using established bioinformatic pipelines.
• Maintain detailed and accurate electronic laboratory notebooks.
• Represent CBP team, and communicate with project discipline leads, and contribute to generation of mechanistic hypothesis through interpretation of proteomics data.
• Ph.D. in Proteomics, Chemical Biology, Biological Sciences, Biochemistry, or a related discipline, with at least 2 years of hands-on proteomics experience, including experience gained during doctoral or postdoctoral research. Or Masters degree with 3-5 years of hands-on proteomics experience.
• Knowledge and hands-on experience with proteomic sample preparation.
• Experience in mammalian cell culture.
• Experience employing cell-based and proteomic assays to understand small molecule mechanisms of action.
• Demonstrated ability to work effectively both independently and collaboratively, contribute to scientific discussions, solve problems, and deliver high-quality results within agreed timelines.

Preferred Background:

• Experience developing or applying proximity ligation assays or related proximity-based protein-protein interaction validation assays.
• Experience using LC-MS/MS platforms and semi-automated instrumentation.
• Experience using proteomics search and analysis tools, such as Spectronaut, Proteome Discoverer, MaxQuant, Skyline and Xcalibur.