Scientist (Non PhD)

Insight Global; San Diego (CA); On-site; USD 85,000 - 110,000; Full time

Job summary

Insight Global is seeking a Scientist (non-PhD) to join our interdisciplinary team supporting and advancing our preclinical oncology drug pipeline. The role emphasizes cellular/molecular biology, in vitro pharmacology, and robust assay development in 2D and 3D formats.

The successful candidate will design and execute studies, implement new technologies, interpret data, and present results. Strong communication and independent problem-solving are essential.

Qualifications

• BS or BS/MS in the biological sciences with 2-4 years of relevant laboratory experience.
• Extensive cell culture experience required.
• Experience in cellular and molecular biology techniques (western blotting, qPCR, CRISPR-Cas9 or si/shRNA, immunofluorescence, immunoprecipitation, cloning/plasmid engineering, etc.).
• Ability to design, optimize, develop and routinely execute robust immune-based screening assays such as ELISA, MSD, HTRF, alphaLISA etc. and functional cell screening assays including Cyquant, CTG, and IncuCyte in medium and high-throughput 2D and 3D formats.
• Ability to program, validate and operate liquid-handling automation including Biomek liquid handler and multi-drop Combi for 96-well and 384-well plate-based assays and use of plate readers such as PHERAStar and Celigo for data generation.
• Ability to work independently and design experiments that test complex hypotheses.
• Attention to detail, and ability to multi-task/ prioritize and manage timing of daily activities.
• Effective oral and written communication skills.
• Ability to work well as part of an interdisciplinary team.
• Fluency in basic data analysis software such as GraphPad, Excel, Activity Base, etc.
• Pharmaceutical industry experience.
• Experience with complex 3D model systems such as matrix-attached models, 3D bioprinting, spheroids and/or organoids in mono- and immune cell co-culture systems.
• Microfluidics and/or tumor-on-a-chip experience.
• Experience with immunofluorescent microscopy and high-content imaging and analysis systems such such as Perkin Elmer Operetta CLS, Signals Image Artist, Cell Profiler, FIJI, etc.
• Experience in generation and/or interpretation of multi-omic datasets, such as RNAseq, ChIPseq, proteomics, or functional genomic screens.
• Preference for candidates with expertise in oncogenic signaling, cell cycle regulation, and/or epigenetic/transcriptional regulation.

Responsibilities

• Design, plan, and execute studies to explore novel drug targets.
• Implement new technologies to support drug discovery and interpret experimental results.
• Present research findings and collaborate across an interdisciplinary team.

Skills

Cell culture

Molecular biology

Assay development

ELISA/HTRF

CRISPR/Cas9

Automation (Biomek)

Data analysis (GraphPad)

High-content imaging

3D culture models

Cancer biology

Education

BS/MS in biological sciences

Tools

Biomek automation

PHERAStar reader

Celigo

Operetta CLS

Job description

Job Description

We are seeking a Scientist (non-PhD) to join our team. The candidate will join our interdisciplinary and highly collaborative team to support and advance our preclinical oncology drug pipeline.

The ideal candidate will have a strong background in cellular and molecular biology, in vitro pharmacology, and biochemical/cell assay development. They will design, plan, and execute studies to explore novel drug targets. They will implement new technologies to support drug discovery, interpret experimental results, and present their research. The successful candidate will have a proven track record of independent research experience with an ability to learn quickly and think critically. This position will provide the motivated candidate with ample opportunity for scientific growth through exposure to all aspects of the drug discovery process. Special consideration will be given for candidates that have expertise in cancer biology.

Skills and Requirements

• BS or BS/MS in the biological sciences with 2-4 years of relevant laboratory experience.
• Extensive cell culture experience required.
• Experience in cellular and molecular biology techniques such as western blotting, qPCR, CRISPR-Cas9 or si/shRNA, immunofluorescence, immunoprecipitation, cloning/plasmid engineering, etc.
• Ability to design, optimize, develop and routinely execute robust immune-based screening assays such as ELISA, MSD, HTRF, alphaLISA etc. and functional cell screening assays including Cyquant, CTG, and IncuCyte in medium and high-throughput 2D and 3D formats.
• Ability to program, validate and operate liquid-handling automation including Biomek liquid handler and multi-drop Combi for 96-well and 384-well plate-based assays and use of plate readers such as PHERAStar and Celigo for data generation.
• Ability to work independently and design experiments that test complex hypotheses.
• Attention to detail, and ability to multi-task/ prioritize and manage timing of daily activities.
• Effective oral and written communication skills.
• Ability to work well as part of an interdisciplinary team.
• Fluency in basic data analysis software such as GraphPad, Excel, Activity Base, etc.
• Pharmaceutical industry experience.
• Experience with complex 3D model systems such as matrix-attached models, 3D bioprinting, spheroids and/or organoids in mono- and immune cell co-culture systems.
• Microfluidics and/or tumor-on-a-chip experience.
• Experience with immunofluorescent microscopy and high-content imaging and analysis systems such such as Perkin Elmer Operetta CLS, Signals Image Artist, Cell Profiler, FIJI, etc.
• Experience in generation and/or interpretation of multi-omic datasets, such as RNAseq, ChIPseq, proteomics, or functional genomic screens.
• Preference for candidates with expertise in oncogenic signaling, cell cycle regulation, and/or epigenetic/transcriptional regulation.

We are a company committed to creating diverse and inclusive environments where people can bring their full, authentic selves to work every day. We are an equal employment opportunity/affirmative action employer that believes everyone matters. Qualified candidates will receive consideration for employment without regard to race, color, ethnicity, religion, sex (including pregnancy), sexual orientation, gender identity and expression, marital status, national origin, ancestry, genetic factors, age, disability, protected veteran status, military or uniformed service member status, or any other status or characteristic protected by applicable laws, regulations, and ordinances. If you need assistance and/or a reasonable accommodation due to a disability during the application or the recruiting process, please send a request to [e-mail].