Scientist, Lentiviral Vector Cell Line Development

asimov; Boston (MA); In loco; USD 90.000 - 130.000; Tempo pieno

Descrizione del lavoro

Asimov, a Boston-based biotech startup, seeks a Scientist to join the Lentiviral Vector Cell Line Development team. You will engineer stable producer cell lines and drive upstream process optimization for LV therapeutics in a fast-paced setting.

The role involves cross-functional collaboration, scale-up across Ambr15/Ambr250, and development of LV analytical assays, with a focus on data-driven design and rigorous record-keeping.

Competenze

• PhD or higher in a related field with hands-on LV production in industry.
• Experience in LV transductions and assays (ddPCR/qPCR, flow cytometry).
• Experience with Ambr15/Ambr250 or benchtop bioreactors.
• Excellent written and verbal communication.

Mansioni

• Perform LV stable producer cell line development including transfection, pool generation, cloning and cell banking.
• Assess LV preparations for quantity, potency and infectivity.
• Support novel workflows and applications in LV CLD.
• Contribute to projects on viral vector therapeutics with quality attributes.
• Collaborate cross-functionally with Synthetic Biology and Process Development teams.
• Assist scale-up and characterization across Ambr systems and LV analytics.
• Design experiments, analyze data, troubleshoot, and maintain records.
• Represent LV CLD on project teams and communicate results clearly.

Conoscenze

LV production

Viral transduction

Flow cytometry

ddPCR/qPCR

Cell line development

Data analysis

Formazione

PhD in Biological/Chemical/Cell/Molecular biology

MS in related field

Bachelor’s with 6–8 years experience

Strumenti

Ambr15

Ambr250

Benchtop bioreactor

Descrizione del lavoro

Our mission at Asimov is to advance humanity's ability to design living systems, enabling biotechnologies with outsized benefit to society. We're developing a mammalian synthetic biology platform––from cells to software––to enable the design and manufacture of next-generation therapeutics.

We are seeking a Scientist to join our Lentiviral Vector Cell Line Development team in Boston, MA. The ideal candidate has a proven track record developing lentiviral vector (LV) packaging and stable producer cell lines in an industry setting. In this role, you will play a central part in engineering stable producer cell lines for LV therapeutics while driving upstream process optimization and scale-up. This is a unique opportunity to work at a nimble, forward-thinking synthetic biology startup and help build the future of biological engineering.

About the Role:

• Perform Lentiviral vector stable producer cell line development processes including transfection, pool generation, cloning, characterization, and cell banking using state-of-the-art equipment.

• Perform assays to assess quantity, potency and infectivity of lentiviral vector preparations.

• Support innovation through development of novel workflows and applications.

• Support research projects to develop viral vector therapeutics with enhanced quality attributes such as infectivity, potency and/or cell targeting.

• Work effectively as part of a cross-functional team in support of the LV CLD platform and in collaboration with Synthetic Biology and Process Development teams.

• Contribute to process development efforts, including scale-up and characterization across Ambr15, Ambr250, and benchtop systems, as well as LV analytical assay development.

• Design experiments, analyze data, troubleshoot, and maintain detailed records.

• Represent LV CLD on project teams, making decisions for cell line development activities and effectively communicating results, progress and plans.

About You:

• You have a PhD in Biological Engineering, Chemical Engineering, Cell Biology, Molecular Biology, or a related field with 0-2 years of hands-on industry experience with Lentiviral vector production and cell line development in support of gene therapies; alternatively, a MS with 3-4 years or Bachelors with 6-8 years of this type of experience.

• Extensive experience performing viral transductions, including T-cell transduction assay, flow cytometry, and quantitative gene expression analyses (ddPCR or qPCR).

• Experience with Ambr15, Ambr250, and/or benchtop scale viral vector bioreactor processes.

• Experience with downstream purification of Lentiviral vectors, viral genome engineering, and/or host cell genome engineering related to virus production or producer cell line technologies is highly desired.

• Strong written and verbal communication skills required.

We're fueled by a vision to transform biological engineering into a fully-fledged engineering discipline. Should you join our team, you will grow with a constantly evolving organization and push the frontiers of synthetic biology. Company culture is key to Asimov, and ours is a culture of recombination; we believe that our mission can only be achieved by bringing together a diverse team with a mixture of backgrounds and perspectives.