Scientist II-Antibody Technologies - Req Flow Cytometry Exp (onsite)

MillenniumSoft Inc; San Diego (CA); On-site; USD 90,000 - 130,000; Full time

Job summary

MillenniumSoft Inc in San Diego, CA seeks a Scientist II in Antibody Technologies to contribute to R&D of monoclonal antibodies. You will work in a dynamic team on multiple workstreams including antibody screening via flow cytometry, hybridoma tissue culture, and recombinant protein expression.

The role requires hands-on flow cytometry, tissue culture expertise, and a foundation in biochemistry and molecular biology; you will design experiments, analyze data, troubleshoot, improve processes, and

Qualifications

• Bachelor’s or Master’s degree in cellular or molecular biology, immunology, cell biology or related field and 2–4 years lab experience.
• Flow cytometry and tissue culture experience required.

Responsibilities

• Design and execute experiments with varying complexity using multi-color Flow Cytometry.
• Analyze data using FlowJo and FCS Express; document results per SOPs.
• Transfect and express antibodies in cell lines; culture hybridomas and other cell lines.
• Operate automated liquid handling and other lab automation equipment.
• Troubleshoot, prioritize tasks and manage daily lab operations.
• Train junior scientists in techniques and processes.
• Meet deadlines and plan follow-up experiments with minimal guidance.

Skills

Flow cytometry

Tissue culture

Data analysis

Scientific writing

Problem solving

Education

BSc or MSc in cellular/molecular biology or immunology

Tools

BD FACS Diva

FlowJo

FCS Express

Microsoft Office

Job description

We are seeking a highly motivated and organized Scientist II to contribute to the research and development of monoclonal antibodies in our Antibody Technologies team. This individual will be responsible for working in a dynamic team environment and will contribute to multiple workstreams including but not limited to antibody screening using flow cytometry, hybridoma tissue culture and development, and recombinant protein expression. The position requires extensive hands-on experience with flow cytometry, expertise in tissue culture, and an understanding of biochemistry and molecular biology. The successful candidate is expected to design and execute experiments, be detail-oriented, analyze and interpret data, contribute to troubleshooting efforts, identify and implement process improvements and will be accountable for preparing and presenting data at lab meetings regarding required tasks and expected results.

Scientist II-Antibody Technologies

We are seeking a highly motivated and organized Scientist II to contribute to the research and development of monoclonal antibodies in our Antibody Technologies team. This individual will be responsible for working in a dynamic team environment and will contribute to multiple workstreams including but not limited to antibody screening using flow cytometry, hybridoma tissue culture and development, and recombinant protein expression. The position requires extensive hands-on experience with flow cytometry, expertise in tissue culture, and an understanding of biochemistry and molecular biology. The successful candidate is expected to design and execute experiments, be detail-oriented, analyze and interpret data, contribute to troubleshooting efforts, identify and implement process improvements and will be accountable for preparing and presenting data at lab meetings regarding required tasks and expected results.

Duties And Responsibilities

• Responsible for the design and execution of experiments with varying complexity using multi-color Flow Cytometry.
• Analyze data using FCS Express and FlowJo software, report test results including completion of all required documentation and database entries as required by protocols and SOPs.
• Responsible for transfection and expression of antibodies in cell lines, hybridoma tissue culture, and culture of various cell lines for testing. Specific requirement for hands-on experience with aseptic technique and cell-culture techniques.
• Responsible for executing experiments using automated liquid handling and other automation equipment
• Day-to-day problem solving and cross-functional team-based activities to ensure that daily operations and timelines are met.
• Contribute to identification and implementation of process improvement projects
• Train junior scientists on laboratory techniques and business processes
• Independently analyze own research data, solve problems with minimal guidance and effectively plan follow up experiments. Will be expected to efficiently prioritize individual workload.
• Involvement with scheduling, operational and maintenance duties required within the laboratory.

Knowledge And Skills

• Hands on experience designing, performing, and analyzing flow cytometry experiments, preferably on BD flow cytometers (LSRII, LSR Fortessa, Canto, Aria).
• Proficient in tissue culture techniques; including transfection of cells for protein expression.
• Software: BD FACS Diva, FlowJo, FCS Express, and Microsoft Office Suite.
• Familiarity with assay automation.
• Familiarity with molecular biology (DNA preparation)
• Effective interpersonal skills and ability to work in a cross-functional team-oriented environment to achieve organizational goals.
• Effective written and oral communication skills.
• Demonstrated ability to follow established policies and procedures.
• Demonstrated ability to keep neat, accurate, and complete records.
• Effective analytical and problem-solving skills.

Education And Experience

A Bachelor’s or Master of Science degree in cellular or molecular biology, immunology, cell biology or related scientific discipline and a minimum of 2-4 years of laboratory experience required. Flow cytometry and tissue culture experience required.