Scientist - Required Flow Cytometry, Tissue Culture & 2 Yrs lab Exp)

MillenniumSoft Inc; San Diego (CA); On-site; USD 28,000 - 41,000; Full time

Job summary

Medical Devices Company in San Diego seeks a mid-level Scientist specialized in flow cytometry and tissue culture to join the Antibody Technologies team. You will contribute to monoclonal antibody development, perform flow cytometry tests, and support hybridoma work and recombinant protein expression.

The role requires hands-on experience with flow cytometry, cell culture, and data analysis, with responsibility for experiments, data presentation, and adherence to lab SOPs.

Qualifications

• Min 2 years lab experience with flow cytometry and tissue culture.
• Experience in monoclonal antibody development is a plus.
• Ability to set up and execute experiments with data presentation.

Responsibilities

• Execute experiments with multi-color flow cytometry.
• Analyze data and report results per SOPs and documentation requirements.
• Transfection and expression of antibodies and recombinant proteins in cell lines; culture of various lines for testing.
• Maintain aseptic technique and cell culture standards.
• Prioritize workload and troubleshoot to meet timelines.
• Assist with scheduling and maintenance duties within the lab.

Skills

Flow cytometry

Tissue culture

Data analysis

Documentation

Education

Bachelor's or Master's in cellular/molecular biology or related field

Tools

BD FACS Diva

FlowJo

FCS Express

MS Office

Job description

Position : Scientist - Required Flow Cytometry, Tissue Culture & 2 Yrs lab Exp)

Location : San Diego, CA

Duration : 12 Months

Total Hours/week : 40 pay rate:$20-30/hr

1st Shift

Client : Medical Devices Company

Job Category : Research & Development

Level of Experience : Mid-Level

Employment Type : Contract on W2 (Need US Citizens or GC Holders or GC EAD or OPT or EAD or CPT)

Work hours: 40 hrs/wk. standard work hours ~8am-5pm.

3 Must haves on the resume: min 2 years lab experience. Flow cytometry experience. Tissue culture experience.

Job Description

• We are seeking a highly motivated and organized Scientist to contribute to the research and development of monoclonal antibodies in our Antibody Technologies team.
• This individual will be responsible for working in a dynamic team environment and be a contributor to multiple work streams, including but not limited to testing new antibodies for use in flow cytometry applications, hybridoma tissue culture and development, recombinant antibody expression.
• The position requires extensive hands-on experience with flow cytometry, expertise in tissue culture, and an understanding of biochemistry and molecular biology.
• The successful candidate is expected to set up and execute experiments, be detail oriented, skilled at data analysis and troubleshooting and will be accountable for preparing and presenting data at lab meetings with guidance regarding required tasks and expected results.

Duties And Responsibilities

• Responsible for the execution of experiments with varying complexity using multi-color Flow Cytometry.
• Analyze data using FCS Express and FlowJo software, report test results including completion of all required documentation and database entries as required by protocols and SOPs.
• Responsible for transfection and expression of antibodies and recombinant proteins in cell lines, hybridoma tissue culture, and culture of various cell lines for testing.
• Specific requirement for hands-on experience with aseptic technique and cell-culture techniques.
• Day-to-day problem solving and cross-functional team-based activities to ensure that daily operations and timelines are met.
• Independently analyze own research data, solve problems with minimal guidance and effectively plan follow up experiments.
• Will be expected to efficiently prioritize individual workload.
• Involvement with scheduling, operational and maintenance duties required within the laboratory.

Knowledge And Skills

• Hands on experience designing, performing, and analyzing flow cytometry experiments, preferably on client flow cytometers (LSRII, LSR Fortessa, Canto, Aria).
• Proficient in tissue culture techniques; including transfection of cells for protein expression.
• Software: BD FACS Diva, FlowJo, FCS Express, and Microsoft Office Suite.
• Familiarity with molecular biology (DNA preparation)
• Preferred: Familiarity with assay automation and automated liquid handlers.
• Effective interpersonal skills and ability to work in a cross-functional team-oriented environment to achieve organizational goals.
• Effective written and oral communication skills.
• Demonstrated ability to follow established policies and procedures.
• Demonstrated ability to keep neat, accurate, and complete records.
• Effective analytical and problem-solving skills.

Education And Experience

• A Bachelor's or Master of Science degree in cellular or molecular biology, immunology, cell biology or related scientific discipline and a minimum of 2-5 years of laboratory experience required. Flow cytometry and/or tissue culture experience required.