Scientist I

247Hire; San Diego (CA); On-site; USD 65,000 - 90,000; Full time

Job summary

247Hire is seeking a Scientist I to advance our non-viral cell and gene therapy platform in San Diego. You will validate novel mRNA and DNA designs, perform cloning, IVT mRNA production, and expression assays across mammalian cell lines.

Strong emphasis on meticulous ELN documentation and data presentation is expected. Requirements include a Bachelor's or Master's in molecular biology with 2+/- years of hands-on experience in cloning, IVT, and molecular assays such as qRT-PCR, Western blot, and

Qualifications

• Bachelor's or Master's in molecular biology or related field with relevant lab experience.
• Proficiency in cloning and IVT mRNA production techniques.
• Experience with reporter assays and RT-qPCR.
• Strong communication and meticulous ELN documentation.

Responsibilities

• Design and clone expression vectors and IVT donor plasmids.
• Perform DNA and mRNA transfections in mouse and human cell lines.
• Assess nucleic acid expression using qPCR, reporter assays, and Western blot.
• Maintain ELN and prepare summary slides.

Skills

Gibson assembly

GoldenGate cloning

site-directed mutagenesis

IVT mRNA prep

qRT-PCR

Western blot

Luciferase assays

Cell culture

ELN documentation

Communication

Education

Bachelor's degree in molecular biology or related field

Master's degree in molecular biology

Tools

SnapGene

Prism

MS Office

Job description

Responsibilities

The Scientist I will be a vital contributor to our non-viral cell and gene therapy delivery platform, primarily focused on R&D for innovative nucleic acid payload technologies. This role involves evaluating novel mRNA and DNA designs using a range of molecular biology techniques, including plasmid cloning, in vitro validation, and the production of in vitro-transcribed mRNA. To verify successful expression, the scientist will utilize assays such as Western blot, qRT-PCR, and luciferase reporter assays.

• Design and clone expression vectors and IVT donor plasmids to support research initiatives.
• Execute DNA and mRNA transfections in mouse and human cell lines.
• Assess nucleic acid expression through molecular methods like qPCR, reporter assays and Western blotting.
• Maintenance of detailed electronic laboratory notebooks (ELN) and preparation of summary slides

Required Skills

• Education/Experience: Bachelor's degree with 2+ years or a Master's degree with 1+ years of experience in molecular biology, pharmaceutical sciences, or a related field.
• Molecular Biology: Required proficiency in advanced cloning (Gibson assembly, Golden Gate, site-directed mutagenesis) and IVT.
• Functional Assays: Hands-on expertise in measuring transgene delivery success via reporter assays and qRT-PCR.
• Communication: Exceptional oral and written skills with a meticulous attention to detail.
• Must haves for this position are:

• Molecular cloning and DNA manipulation techniques (e.g. Gibson assembly, GoldenGate cloning, site-directed mutagenesis)
• Work with RNA, especially preparing in vitro-transcribed mRNA
• Experience with molecular assays to assess gene - e.g. Western blot, luciferase reporter assays, qRT-PCR)

Preferred Skills

• Cell Culture: Preferred experience in genetic manipulation of cell lines and primary cells (e.g., T cells, monocytes, hepatocytes).
• Software/Tools: Desired proficiency in Snapgene, Prism, and MS Office.