Research Technician I, Oral Biology

University at Buffalo; Buffalo (NY); Sur place; USD 42 000 - 64 000; Plein temps

Résumé du poste

University at Buffalo is seeking a Research Technician I to support a dental pulp stem cell project in the Department of Oral Biology. The role involves executing mammalian cell culture, molecular biology techniques, histology and imaging, and flow cytometry.

The candidate will assist with animal work, data analysis, and administrative duties, under supervision, with opportunities to train and supervise newer staff and students.

Qualifications

• Experience with mammalian cell culture.
• Experience with animal work is a strong plus.
• Ability to manage multiple tasks with minimal supervision.
• Proficiency in molecular biology techniques and histology.

Responsabilités

• Mammalian cell culture.
• DNA/RNA extraction and PCR.
• Protein detection/quantification and cell signaling studies (immunofluorescence, western blotting, ELISA, plasmid recombination).
• Mice and Ferret: colony maintenance, breeding, genotyping, sample harvesting.
• Histology (sample preparation).
• Microscopy imaging (brightfield, confocal, TEM).
• Flow cytometry: multi-channel FACS and immune profiling.
• Administrative duties (notes, purchasing, inventory, protocol submissions).
• Training and supervision of new technicians and students.
• Experience with GraphPad Prism is preferred.

Connaissances

Mammalian cell culture

DNA/RNA extraction

PCR

Protein detection/quantification

Immunofluorescence

Western blotting

ELISA

Plasmid recombination

Mice/ferret care

Histology

Microscopy imaging

Flow cytometry

Administrative duties

Training supervision

GraphPad Prism

Outils

GraphPad Prism

Description du poste

A Research Technician I position is available at the University at Buffalo School of Dental Medicine's Department of Oral Biology with Dr. Camila Sabatini's research team. The primary objective of this position is to study dental pulp stem cells, with a focus on regenerative dentistry. The successful candidate should be able to manage multiple detail-oriented tasks with minimal supervision, capable of trouble-shooting and critical thinking, develop and execute in vitro and in vivo experiments, histological processing and imaging, molecular biology techniques, perform administrative tasks, self-motivated, dedicated and creative. Excellent organizational skills, ability to work well with others, and willingness to learn new lab techniques and follow instructions are key. Prior experience with animal work is a strong plus.
The activities of the Research Technician I will consist of:

• Mammalian cell culture;
• DNA/RNA extraction and PCR;
• Protein detection/quantification and cell signaling studies (immunofluorescence, western blotting, ELISA, plasmid recombination);
• Mice and Ferret: maintaining the colony, breeding and genotyping, and sample harvesting;
• Histology (sample preparation);
• Microscopy imaging (brightfield, confocal, and transmission electron microscopy);
• Flow cytometry: multiple channel FACS analysis and immune profiling;
• Administrative duties (taking notes during lab meetings, assisting with purchase orders and inventory management, and managing protocol submissions and documentation, lab organization);
• Training and supervision of new technicians and students;
• Requires experience with statistical software Graphpad Prism.
  

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The Research Foundation of SUNY is an EOE and welcomes all to apply including veterans and individuals with a disability.