Research Technician I

Mass General Brigham; Boston (MA); On-site; USD 45,000 - 75,000; Full time

Job summary

An established industry player is seeking a Research Technician I to join their dynamic team at a leading research center. In this role, you'll perform essential laboratory procedures, including mouse colony management and molecular biology techniques, contributing to groundbreaking research in wound healing and inflammation. The laboratory environment is collaborative and well-equipped, fostering innovation and discovery. If you have a passion for research and a strong background in wet lab techniques, this is an exciting opportunity to advance your career while making a meaningful impact in the field of medical science.

Qualifications

• Bachelor's degree required with wet lab experience preferred.
• Proficient in molecular biology and biochemistry procedures.

Responsibilities

• Manage mouse colonies and perform routine molecular biology tasks.
• Prepare solutions and conduct assays including RT-PCR and western blots.

Skills

Wet Lab Experience

Technical Ability

Excellent Communication Skills

Independent Work

Group Collaboration

Education

Bachelor of Science/Art

Tools

RT-PCR

Western Blots

Cell and Tissue Culture

Microscopy

Flow Cytometry

Job description

Site: The General Hospital Corporation

At Mass General Brigham, we know it takes a surprising range of talented professionals to advance our mission—from doctors, nurses, business people and tech experts, to dedicated researchers and systems analysts. As a not-for-profit organization, Mass General Brigham is committed to supporting patient care, research, teaching, and service to the community. We place great value on being a diverse, equitable and inclusive organization as we aim to reflect the diversity of the patients we serve.

At Mass General Brigham, we believe a diverse set of backgrounds and lived experiences makes us stronger by challenging our assumptions with new perspectives that can drive revolutionary discoveries in medical innovations in research and patient care. Therefore, we invite and welcome applicants from traditionally underrepresented groups in healthcare — people of color, people with disabilities, LGBTQ community, and/or gender expansive, first and second-generation immigrants, veterans, and people from different socioeconomic backgrounds – to apply.

Job Summary

The Research Technician I will perform standard laboratory procedures in the laboratory of Dr Marneros at the Cutaneous Biology Research Center/MGH.

Duties include:

• Mouse colony management and husbandry including Tag and tail, genotyping, and euthanasia and tissue harvest, as well as phenotypic characterization of mouse phenotypes.
• Routine molecular biology, histology/immunochemistry, microscopy and flow cytometry in support of projects in cutaneous biology and immunology.

Our laboratory is investigating molecular mechanisms involved in wound healing, angiogenesis and inflammation.

Qualifications

• Wet Lab Experience Required
• Please submit your Unofficial Transcript
• Submit Letters of Recommendation

Project 1: We investigate the role of proangiogenic factors for wound healing and age-related diseases. We could show that the proangiogenic factor VEGF-A induces oxidative stress and NLRP3 inflammasome activation to promote age-related diseases (Cell Reports, 2013; FASEB J, 2014; EMBO Mol Med, 2016; FASEB J, 2018, eLife, 2020). We use a variety of in vivo and in vitro approaches to investigate a novel pathogenic link between VEGF-A and NLRP3 inflammasome activation.

Project 2: We identified a novel pathway that is critical for epithelial differentiation, especially in the kidney and skin. This new pathway plays a critical role in kidney and skin development (Dev Cell, 2020; Cell Reports, 2021; J Clin Invest, 2024). We are exploring key novel molecular mechanisms that regulate skin and kidney development, with implications for several skin and kidney diseases.

In summary, our laboratory uses a large number of diverse experimental approaches (human genetics, mouse genetics, chemical screens, in vitro assays, cutting-edge imaging technology) to define novel mechanisms in angiogenesis, wound healing and inflammation. All of our projects have strong translational clinical relevance.

Our laboratory is embedded in a highly productive and well-equipped environment at the Cutaneous Biology Research Center of Massachusetts General Hospital/Harvard Medical School. Cutting-edge technologies are available within our Department and the MGH/Harvard research community.

lab website: https://www.massgeneral.org/dermatology/research/cutaneous-biology-research-center/faculty-labs/alexander-marneros-lab

Principal Duties and Responsibilities

• Prepares basic solutions and performs base-level procedures as assigned (i.e. Pipetting, cell and tissue culture, molecular biology and biochemistry procedure etc).
• Routine maintenance work for supporting the laboratory such as general laboratory upkeep.
• Perform routine assays, including RT-PCR, western blots, tissue processing, stainings and immunolabeling.
• Conduct analysis of results.
• Assist in procedures with rodent models.
• Maintain a laboratory notebook.
• Attend weekly laboratory meetings and maintain laboratory notebooks.
• Perform other duties as assigned.

Skills/Abilities

• Must exhibit initiative and be able to work independently when trained.
• Must have technical ability, be proficient, and accurate in scientific experimentation.
• Ability to work in a group environment and assist with multiple research projects.
• Excellent written and verbal communication skills.

Education

• Bachelor of Science/Art required.

Experience

• Prior laboratory experience preferred, especially in working with mice.

Additional Job Details (if applicable)

Remote Type

Onsite

Work Location

149 Thirteenth Street Building 149

Scheduled Weekly Hours

40

Employee Type

Regular

Work Shift

Day (United States of America)

EEO Statement:

The General Hospital Corporation is an Affirmative Action Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.

Mass General Brigham Competency Framework

At Mass General Brigham, our competency framework defines what effective leadership “looks like” by specifying which behaviors are most critical for successful performance at each job level. The framework is comprised of ten competencies (half People-Focused, half Performance-Focused) and are defined by observable and measurable skills and behaviors that contribute to workplace effectiveness and career success. These competencies are used to evaluate performance, make hiring decisions, identify development needs, mobilize employees across our system, and establish a strong talent pipeline.