Research Technician Chouchani Lab

danafarber; Boston (MA); On-site; USD 48,000 - 51,000; Full time

Job summary

Located in Boston, Dana-Farber Cancer Institute seeks a research assistant to support Dr. Edward Chouchani's Lab, performing molecular and cell biology experiments and assisting with cell-based assays.

Duties include maintaining cell lines, handling laboratory animals, protein purification, and ordering lab supplies; candidates gain exposure to qPCR, western blot, sequencing, and related techniques. This role offers continuous learning opportunities and a solid stepping stone for those pursuing

Qualifications

• Bachelor's or Master's degree in Biology, Biotechnology, Chemistry, or a related field.
• Hands-on laboratory experience in molecular and/or cell biology.
• Previous experience working in a medical or scientific research setting is preferred.
• Must be self-motivated and an independent worker with excellent communication skills (written and verbal).
• At least two years of commitment is required.

Responsibilities

• Assisting postdocs and students with cell based assays, which will involve maintenance of cell lines and perform routine molecular and cellular biology experiments (e.g., qPCR, western blot, sequencing, plasmid purification and bacterial transformation).
• Assisting with biochemical techniques such as protein purification and quantification, enzyme assays and mass spectrometry-based protein and metabolite measurements.
• Maintenance of Chouchani Lab animal (laboratory mouse) colony, including weaning, tail biopsies, DNA preparation and genotyping by PCR.
• Placing orders for the purchase of lab consumables.
• General lab management (meaning keep things running and functional, organized and stocked).

Job description

Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative
researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining
extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive
care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to
underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.

This position in Dr. Edward Chouchani's Lab will assist scientists in performing scientific research of varying complexity. Duties include
working with laboratory mice and conducting basic molecular biology and cell biology techniques. Basic procedures include but are not limited
to: pipetting; cell and tissue culture, handling of small laboratory animals, etc. There will be continuous learning opportunities and the position
will be a good stepping stone for those interested in biomedical research.

Located in Boston and the surrounding communities, Dana-Farber Cancer Institute is a leader in life changing breakthroughs in cancer research and patient care. We are united in our mission of conquering cancer, HIV/AIDS, and related diseases. We strive to create an inclusive, diverse, and equitable environment where we provide compassionate and comprehensive care to patients of all backgrounds, and design programs to promote public health particularly among high-risk and underserved populations. We conduct groundbreaking research that advances treatment, we educate tomorrow's physician/researchers, and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.

Responsibilities

• Assisting postdocs and students with cell based assays, which will involve maintenance of cell lines and perform routine molecular and cellular biology experiments (e.g., qPCR, western blot, sequencing, plasmid purification and bacterial transformation).
• Assisting with biochemical techniques such as protein purification and quantification, enzyme assays and mass spectrometry-based protein and metabolite measurements.
• Maintenance of Chouchani Lab animal (laboratory mouse) colony, including weaning, tail biopsies, DNA preparation and genotyping by PCR.
• Placing orders for the purchase of lab consumables.
• General lab management (meaning keep things running and functional, organized and stocked).

Qualifications

• Bachelor's or Master's degree in Biology, Biotechnology, Chemistry, or a related field.
• Hands-on laboratory experience in molecular and/or cell biology.
• Previous experience working in a medical or scientific research setting is preferred.
• Must be self-motivated and an independent worker with excellent communication skills (written and verbal).
• At least two years of commitment is required.

Preferred Qualifications

• Prior experience with protein purification, structural biology, biochemical enzyme assays, and mass spectrometry
• Prior experience with T cells or other immune cells (e.g., primary T cells from PBMCs or other systems)
• Familiarity with standard molecular biology techniques (e.g., PCR, western blot, cloning)
• Experience with genetic sequencing and basic data analysis.

SUPERVISORY RESPONSIBILITIES

No supervisory responsibilities. Normally receives detailed instructions on all work.

PATIENT CONTACT

None

At Dana-Farber Cancer Institute, we work every day to create an innovative, caring, and inclusive environment where every patient, family, and staff member feels they belong. As relentless as we are in our mission to reduce the burden of cancer for all, we are committed to having faculty and staff who offer multifaceted experiences. Cancer knows no boundaries and when it comes to hiring the most dedicated and compassionate professionals, neither do we.

Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other characteristics protected by law.

The hiring range is based on market pay structures, with individual salaries determined by factors such as business needs, market conditions, internal equity, and based on the candidate's relevant experience, skills and qualifications.

For union positions, the pay range is determined by the Collective Bargaining Agreement (CBA).

$47,500.00 - $51,300.00