Research Specialist A/B

Trustees of University of Pennsylvania; Arizona; Presencial; USD 45.000 - 60.000; Jornada completa

Descripción de la vacante

The Amado Laboratory at the Perelman School of Medicine, University of Pennsylvania, seeks a Research Specialist A/B to advance gene-therapy approaches for ALS and neurodegenerative disorders. You will design and run experiments using cell lines, patient-derived cells, and mouse models in a collaborative setting.

We value strong organization, record-keeping, and the ability to learn routines quickly. Prior work with mammalian cell culture and basic molecular biology is preferred, with

Formación

• Bachelor of Science required; 0–2 years experience for RS-A/B positions.
• Experience with mammalian cell culture and basic molecular biology is preferred.
• Ability to work independently and as part of a team; strong organizational skills.

Responsabilidades

• Design, conduct, and analyze experiments in cell lines, patient-derived cells, and mouse models.
• Contribute to gene therapy projects including delivery, RNAi, gene editing, and ASOs.
• Assist in generating figures and writing Methods for publications.

Conocimientos

Independent work

Team collaboration

Organization & record-keeping

Educación

Bachelor of Science

Herramientas

DNA/RNA/protein extraction

QPCR

Western blotting

Molecular cloning

PCR

Cell culture

Descripción del empleo

University Overview

The University of Pennsylvania, the largest private employer in Philadelphia, is a world-renowned leader in education, research, and innovation. This historic, Ivy League school consistently ranks among the top 10 universities in the annual U.S. News & World Report survey. Penn has 12 highly-regarded schools that provide opportunities for undergraduate, graduate and continuing education, all influenced by Penn's distinctive interdisciplinary approach to scholarship and learning. As an employer Penn has been ranked nationally on many occasions with the most recent award from Forbes who named Penn one of America's Best Large Employers in 2023.

Penn offers a unique working environment within the city of Philadelphia. The University is situated on a beautiful urban campus, with easy access to a range of educational, cultural, and recreational activities. With its historical significance and landmarks, lively cultural offerings, and wide variety of atmospheres, Philadelphia is the perfect place to call home for work and play.

The University offers a competitive benefits package that includes excellent healthcare and tuition benefits for employees and their families, generous retirement benefits, a wide variety of professional development opportunities, supportive work and family benefits, a wealth of health and wellness programs and resources, and much more.

Posted Job Title

Research Specialist A/B

Job Profile Title

Research Specialist A

Job Description Summary

The Amado Laboratory, a part of both the Perelman School of Medicine at the University of Pennsylvania and the Children's Hospital of Philadelphia, is seeking a highly motivated Research Specialist A/B to join our research program focused on the development of gene therapy approaches to treat ALS and related neurodegenerative disorders.

Job Description

Our lab uses cellular and mouse models of disease to identify therapeutic targets and develop cutting-edge gene therapy-based approaches to treat them, including AAV-mediated gene delivery, RNA interference, gene editing, and ASOs. We are seeking someone who can help design, conduct, and analyze experiments in immortalized cell lines, patient-derived stem cells, and mouse models of diseases of the nervous system in a highly collaborative, fun environment. The ideal candidate is comfortable with basic existing lab techniques and is also keen on reading published literature to identify the most effective, reliable, and novel strategies to advance the projects' success, and is not afraid to try new things and troubleshoot. Excellent organization and record-keeping skills are required. Prior experience with the following techniques is desired:

• DNA/RNA/protein extraction
• QPCR
• western blotting
• molecular cloning
• PCR
• cell culture including maintenance and transfection

Prior experience with mouse handling and knowledge of or willingness to learn tissue extraction techniques (especially brain, spinal cord, and/or peripheral nerve) and behavior testing is preferred, as there is a significant in vivo component to our work.

Sectioning/immunohistochemistry/immunofluorescence experience is a bonus, as is experience with imaging acquisition and analysis. For Research Specialist B applicants, duties will be more autonomous with a greater level of independence. Further, although both positions are expected to lead to inclusion in publications from the lab, a Research Specialist B will also be expected to assist in generating figures, writing Methods, and other aspects of manuscript preparation.

Qualifications

RS-A: Bachelor of Science and 0 to 1 year of experience or equivalent combination of education and experience is required. Prior experience with mammalian cell culture (including transfection) and molecular/cell biology is preferred. Prior experience working with mice is preferred; openness to it is required. Ability to work both independently and as part of a team are required. Excellent organizational, time management, and critical thinking skills are required. RS-B: Bachelor of Science and 1 to 2 years of experience or equivalent combination of education and experience is required. Prior experience with mammalian cell culture (including transfection) and molecular/cell biology is required. Prior experience with mice is strongly preferred. Position is contingent upon continued funding.

Job Location - City, State

Philadelphia, Pennsylvania

Department / School

Perelman School of Medicine

Pay Range

$45,278.00 - $60,202.00 Annual Rate Salary offers are made based on the candidate's qualifications, experience, skills, and education as they directly relate to the requirements of the position, and in alignment with salary ranges based on external market data for the job's level. Internal organization and peer data at Penn are also considered.

Equal Opportunity Statement

The University of Pennsylvania is an equal opportunity employer. Candidates are considered for employment withou