Research Scientist (1 Year Fixed Term)

Stanford University School of Medicine; Palo Alto (CA); On-site; USD 76,000 - 80,000; Full time

Job summary

The Stanford Division of Pediatric Radiology within the Department of Radiology is seeking a Research Scientist to perform defined research projects and independently conduct experiments in the Thakor Laboratory, which focuses on translational islet transplantation using pulsed focused ultrasound to improve engraftment and function.

The role involves cell culture, molecular biology, sequencing, ELISA, microscopy, flow cytometry, animal work, data analysis, and contribution to manuscripts and

Qualifications

• Ph.D. required in molecular biology, immunology, stem cell biology, or related field.
• Extensive molecular biology experience and mouse handling.
• Experience with flow cytometry and cell sorting.
• Experience with RNA sequencing and microscopy.
• Ability to independently design and troubleshoot experiments.
• Excellent communication and organizational skills.
• Ability to work independently and collaboratively.
• Knowledge of Microsoft Office and data analysis tools.

Responsibilities

• RNA/DNA extraction.
• RNA sequencing.
• ELISA.
• Cell culture.
• Islet biology.
• Genetic modification techniques.
• Mouse handling.
• Flow-cytometry analysis and sorting.
• Microscopy, protocol optimization (confocal microscopy - preferred).
• Novel assay development.
• Attendance at weekly lab meetings.
• To contribute to multiple projects and to make scientific and written contributions to progress reports, grant applications, and manuscripts.
• Other duties may also be assigned.

Skills

Molecular biology

Mouse handling

Flow cytometry

Cell culture

RNA sequencing

Microscopy

Genetic modification

CRISPR/Cas9

Experiment design

Data analysis

Scientific writing

Communication

Team collaboration

R

GraphPad Prism

Bioinformatics

Immunology

Education

Ph.D. in molecular biology, immunology, stem cell biology, or related field

Tools

Microsoft Office

Adobe Illustrator

GraphPad Prism

R

Job description

Job Description The Stanford Division of Pediatric Radiology within the Department of Radiology is seeking a Research Scientist to perform basic functions and activities involved in defined research projects, and to independently conduct and analyze experiments. The work will be carried out in the laboratory of Dr. Thakor Avnesh. The Thakor Laboratory conducts translational islet transplantation research, leveraging pulsed focused ultrasound (pFUS) to modulate the tissue microenvironment, optimize islet delivery, and improve engraftment and function in both preclinical and clinical settings. We are seeking highly motivated individuals who are passionate about science, learning, and collaboration. Candidates with a strong background in molecular and cellular biology, and/or experience in bioinformatics, will find this an ideal environment to apply and develop their skills. Duties involve culture and maintenance of stem cells and human/mouse islets; standard molecular biology techniques including DNA and RNA extraction, quantitative PCR, ELISA; microscopy; flow cytometry; immunostaining; and experience with animals (i.e., mouse handling, organ collection). Experience in genetic modification techniques, including gene editing (e.g., CRISPR/Cas9), with interest or experience in knock-in and knock-out approaches, and generation/validation of modified cell lines or animal models, in addition to the listed responsibilities is desirable. The candidate will be responsible for leading scientific projects, including design and execution of experiments, analysis and presentation of data, and contributions to manuscript, report, and grant preparation. The candidate will also be expected to optimize existing protocols in collaboration with other lab members. Additional tasks will include maintaining detailed records of experiments and reagent stocks. This position is for a 1-year fixed-term, with the possibility of renewal based on funding, performance, and operational needs.Duties and responsibilities include*:

• RNA/DNA extraction.
• RNA sequencing.
• ELISA.
• Cell culture.
• Islet biology.
• Genetic modification techniques.
• Mouse handling.
• Flow-cytometry analysis and sorting.
• Microscopy, protocol optimization (confocal microscopy - preferred).
• Novel assay development.
• Attendance at weekly lab meetings.
• To contribute to multiple projects and to make scientific and written contributions to progress reports, grant applications, and manuscripts.
• Other duties may also be assigned.

Qualifications Required Qualifications

• Ph.D. in molecular biology, immunology, stem cell biology, or related field.
• Extensive experience with molecular biology, mouse handling and procedures, flow-cytometry analysis and cell sorting, mammalian cell culture, RNA-sequencing, and microscopy.
• Ability to independently design and troubleshoot experiments.
• Excellent communication and organization skills.
• Ability to work both independently and collaboratively.
• Working knowledge of Microsoft Office, Adobe Illustrator, GraphPad Prism, R, and other.

Essential job duties require frequent standing, walking, twisting, bending, stooping, squatting, grasping (light to forceful), fine manipulation, desk-based computer, and writing, with the ability to lift up to 50 pounds and work on hard surfaces for up to eight hours; occasional sitting, kneeling, crawling, reaching overhead, and sorting/file paperwork; rare climbing, scrubbing, sweeping, mopping, chopping, or operating hand/foot controls; correctible vision; ability to perform repetitive motion and work in a dusty, dirty, and odorous environment; and the provision of reasonable accommodations for disabilities as required by law. The expected pay range for this position is $76,383 to $80,000 per annum. Stanford University provides pay ranges representing its good faith estimate of the salary or hourly wage the university reasonably expects to pay for a position upon hire. The pay offered to a selected candidate will be determined based on factors such as (but not limited to) the scope and responsibilities of the position, the qualifications of the selected candidate, departmental budget availability, internal equity, geographic location, and external market pay for comparable jobs. At Stanford University, base pay represents only one aspect of the comprehensive rewards package. The Cardinal at Work website (https://cardinalatwork.stanford.edu/benefits-rewards) provides detailed information on Stanford's extensive range of benefits and rewards offered to employees. Specifics about the rewards package for this position may be discussed during the hiring process. Consistent with its obligations under the law, the University will provide reasonable accommodations to applicants and employees with disabilities. Applicants requiring a reasonable accommodation for any part of the application or hiring process should contact Stanford University Human Resources by submitting a contact form. Stanford is an equal employment opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, protected veteran status, or any other characteristic protected by law. The job duties listed are typical examples of work performed by positions in this job classification and are not designed to contain or be interpreted as a comprehensive inventory of all duties, tasks, and responsibilities. Specific duties and responsibilities may vary depending on department or program needs without changing the general nature and scope of the job or level of responsibility. Employees may also perform other duties as assigned.