Research Lab Tech I - Hematology Hollenhorst Lab

Mass General Brigham Incorporated; Boston, Northern (MA, KY); On-site; USD 29,000 - 40,000; Full time

Job summary

Mass General Brigham Incorporated seeks a Research Lab Tech I for the Hollenhorst Lab in Boston. The role involves performing Western blot, immunoprecipitation, and protein purification, with mass spectrometry sample preparation as needed.

You will work under supervision, maintain notebooks, and contribute to data analysis and presentations. Ideal candidates have 0–1 year of wet-lab experience, strong organization, and an eagerness to learn.

Qualifications

• Bachelor’s degree required; biology/chemistry/biochemistry preferred.
• 0–1 years of relevant laboratory experience; prior training helpful but not required.
• Strong organization, independence and ability to troubleshoot lab protocols.
• Excellent written and oral communication; ability to present data clearly.

Responsibilities

• Perform routine and non-routine experiments (western blot, IP, protein purification) with increasing independence.
• Process platelets and plasma samples; maintain long-term cultures of human cells.
• Maintain electronic lab notebook and manage data; coordinate with PI on experimental design.

Skills

Excel

FlowJo

GraphPad Prism

Data analysis

Teamwork

Communication skills

Education

Bachelor’s degree in biology or related field

Tools

Western blot

Immunoprecipitation

Protein purification

Mass spectrometry sample prep

Flow cytometry

Job description

## Research Lab Tech I - Hematology Hollenhorst LabApplyremote type: Hybridlocations: Boston-MAtime type: Full timeposted on: Posted Yesterdayjob requisition id: RQ4060863Site: The Brigham and Women's Hospital, Inc.Mass General Brigham relies on a wide range of professionals, including doctors, nurses, business people, tech experts, researchers, and systems analysts to advance our mission. As a not-for-profit, we support patient care, research, teaching, and community service, striving to provide exceptional care. We believe that high-performing teams drive groundbreaking medical discoveries and invite all applicants to join us and experience what it means to be part of Mass General Brigham.**Job Summary**The Hollenhorst Lab applies innovative biochemical strategies to study clinically relevant questions regarding the proteins that regulate bleeding and clotting. The goal is to further our understanding of the basic biology of hemostasis and thrombosis with an eye towards questions directly relevant to the care of patients. We are looking for an enthusiastic and motivated individual to perform experiments under the supervision of the principal investigator or senior lab member. The selected candidate will perform western blots, immunoprecipitation, protein purification, and prepare samples for mass spectrometry analysis. The individual will work with a high degree of independence, organize notebooks and manage data, and perform other laboratory experiments relevant for understanding platelets and coagulation factors. Ideal candidates will have training in wet lab techniques, strong organizational skills, and be motivated to further their career by learning and adopting new skills. While applicants from diverse backgrounds and career stages are welcome, this position is a particularly good fit for individuals who seek a 2-year research experience prior to applying to PhD and/or MD programs. The principal investigator will provide direct mentorship and long-term career advice. For individuals with an interest in gaining clinical experience in advance of applying to medical school, the principal investigator will provide shadowing opportunities. \*\*\* When applying, please submit a cover letter along with your resume and include your GPA. Applications that do not include both components will NOT be considered. \*\*\***Qualifications**Principal Duties and Responsibilities• Perform routine and non-routine experiments, which are moderate to complex, with increasing independence. These include western blot, immunoprecipitation, protein purification by liquid chromatography, flow cytometry, and preparation of samples for mass spectrometry analysis. (Prior training in these techniques is helpful but is NOT required.)• Process platelets and plasma samples from donors and patients.• Maintain long-term tissue cultures of human cell lines, monitor cells for viability and contamination.• Maintain a detailed electronic laboratory notebook to reproduce experiments precisely.• Consult with the principal investigator and implement adjustments in experimental design.• Prepare laboratory reagents and chemicals, organize storage. Meticulously maintain and update databases of laboratory supplies and storage. Order new supplies on time to maintain inventory.• Perform routine maintenance and calibration of laboratory equipment, if necessary.• Become proficient in Excel, FlowJo, and GraphPad Prism as needed analyze and critically evaluate data.• Present data and relevant research publications in lab meetings. Help to prepare presentations and written articles for publication.• All other duties, as assigned.Working Conditions• Laboratory environment with frequent work with toxic chemicals, biohazards, bloodborne pathogens, and substances emitting unpleasant odors. • Work requires frequent standing, walking and frequent lifting or carrying supplies.QualificationsRequired:* Bachelor’s degree required, preferably in biology, chemistry or a related science field.* 0-1 yrs of experience. Previous experience (6 months+) with techniques in experimental biology, chemistry and/or biochemistry preferred. Skills/abilities/competencies required:• A positive attitude and eagerness to learn. • Comfortable with working independently and in a team. Good oral and written communication skills. • Ability to learn and perform a variety of laboratory protocols, to identify potential problems and troubleshoot solutions. • Strong organizational skills. • Ability to logically present data. • Sound judgment regarding time-management and laboratory safety.**Additional Job Details (if applicable)**Research Environment The Hollenhorst laboratory is located in the Harvard Institutes of Medicine building, 4 Blackfan Circle, in the center of the Longwood Medical Area. Our primary affiliations are the Brigham and Women’s Hospital Division of Hematology and Harvard Medical School. The laboratory works closely with the National Center for Functional Glycomics at Beth Israel Deaconess Medical Center and is affiliated with the Broad Institute of Harvard and MIT, the Dana-Farber/Harvard Cancer Center and the Harvard Chemical Biology PhD Program. These institutions and programs house scientific and clinical experts in a wide variety of areas, enabling interactions with a myriad of collaborators and communities. We have access to state-of-the-art core facilities for glycomics, proteomics, flow cytometry, etc. These rich resources allow laboratory members to thrive in a supportive and well-equipped environment.**Remote Type**Hybrid**Work Location**45 Francis Street**Scheduled Weekly Hours**40**Employee Type**Regular**Work Shift**Day (United States of America)**Pay Range**$21.00 - $29.01/Hourly**Grade**5At Mass General Brigham, we believe in recognizing and rewarding the unique value each team member brings to our organization. Our approach to determining base pay is comprehensive, and any offer extended will take into account your skills, relevant experience if applicable, education, certifications and other essential factors. The base pay information provided offers an estimate based on the minimum job qualifications; however, it does not encompass all elements contributing to your total compensation package. In addition to competitive base pay, we offer comprehensive benefits, career advancement opportunities, differentials, premiums and bonuses as applicable and recognition programs designed to celebrate your contributions and support your professional growth. We invite you to apply, and our Talent Acquisition team will provide an overview of your potential compensation and benefits package.**EEO Statement:**2200 The Brigham and Women's Hospital, Inc. is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment. To ensure reasonable accommodation for individuals protected by Section 503 of the Rehabilitation Act of 1973, the Vietnam Veteran’s Readjustment Act of 1974, and Title I of the Americans with Disabilities Act of 1990, applicants who require accommodation in the job application process may contact Human Resources at (857)-282-7642.