Research Assistant | Laboratory of Cell Cycle Genetics

The Rockefeller University; New York (NY); On-site; USD 50,000 - 55,000; Full time

Job summary

An established industry player is seeking a motivated individual with a Bachelor of Science degree to join their innovative project focused on developing single-chain antibodies against coronaviruses. This role involves preparing DNA, transforming yeast, and analyzing next-generation sequencing data. You will be part of a collaborative team dedicated to advancing research in virology and antibody development. If you are passionate about molecular biology and eager to contribute to groundbreaking research, this position offers a unique opportunity to make a significant impact in the field.

Qualifications

• Basic laboratory experience in molecular biology and microbiology preferred.
• Experience with DNA preparation and microbial culture is a plus.

Responsibilities

• Prepare plasmid and PCR-generated DNA for yeast libraries.
• Perform high-efficiency yeast transformation to generate libraries.
• Assist with biochemical characterization of single-chain antibodies.

Skills

Molecular Biology

Microbiology

DNA Preparation

PCR Techniques

Next-Generation Sequencing

Education

Bachelor of Science

Tools

Magnetic Bead Technology

Job description

Organization Overview

We work on the cell cycle using budding yeast as a model system. Our collaboration focuses on constructing ordinary differential equation-based models to control the yeast cell cycle, and testing these models genetically and biochemically. We conduct biochemical and genetic screens for binding partners and phosphorylation targets specific to different cyclin-Cdk complexes. Additionally, we plan a combined computational and molecular approach to analyze promoter elements important in both mitotic and meiotic cell cycles.

Project Overview

The overall purpose of the project is to search for broadly cross-reactive single-chain antibodies against viruses in the coronavirus family. Specifically, the work involves:

• Constructing libraries of yeast expressing single-chain antibodies against multiple viral antigens in the coronavirus family
• Screening these libraries for binding and cross-binding to antigens from related viruses
• Preparing DNA and analyzing next-generation sequencing data to determine sequences and specificities of cross-binding versus specific antibodies

Responsibilities

Responsibilities include:

• Preparing plasmid and PCR-generated DNA for constructing yeast libraries
• Performing high-efficiency yeast transformation to generate complex libraries
• Selecting differential binding clones using magnetic bead technology
• Purifying DNA from selected populations and preparing for next-generation sequencing
• Assisting with biochemical and structural characterization of resultant single-chain antibodies
• Performing related duties as assigned or requested

Qualifications

A Bachelor of Science degree with basic laboratory experience is required. Specific experience in molecular biology and microbiology (DNA preparation and analysis, media preparation, microbial culture) is strongly preferred.

The Rockefeller University is an equal opportunity employer – veterans and individuals with disabilities. Qualified applicants will receive consideration for employment without regard to characteristics protected by applicable law, including disability and veteran status.

Compensation

The salary range for this role is:

• Minimum: USD $50,000.00 per year
• Maximum: USD $55,000.00 per year