Research Assistant IV - Pediatric Surgery - Bondoc Lab

CO00 Children's Hospital Medical Center; United States; On-site; USD 58,000 - 75,000; Full time

Job summary

Cincinnati Children's Bondoc Laboratory seeks a Research Assistant to support pediatric liver cancer and development research using human iPSCs, organoid models, and animal studies. You will perform complex experiments, analyze data, and contribute to collaborative projects in a dynamic team.

The role involves mammalian cell culture, molecular assays, histology, and advanced imaging, with opportunities to train others and participate in publications.

Qualifications

• Bachelor's degree or Master's with relevant experience in molecular biology or cellular biology.
• Experience in mammalian cell culture and human iPSC culture preferred.
• Knowledge of organoid culture systems is desirable.

Responsibilities

• Design, perform, troubleshoot and optimize laboratory experiments.
• Execute protocols and adapt to project objectives.
• Analyze and interpret experimental data and maintain records.
• Contribute to publications and presentations.
• Train students and junior staff.

Skills

iPSC culture

Organoid culture

Flow cytometry

Immunostaining

Confocal microscopy

qPCR

Western blotting

Mouse handling

Education

Bachelor's degree in related field

Master's degree in related field

Tools

Histology

Cell sorting

Job description

Primary Location Burnet Campus Department Pediatric Surgery Shift Day (United States of America) Schedule Full time Weekly Hours 40 FTE 1 Employee Status Regular

*Expected Starting Pay Range $58,406.40 - $74,588.80 *Starting pay is based on experience, skills, and equity; exceptions may apply for highly qualified candidates. Additional pay (e.g., shift, on-call, or weekend differentials) and benefits may apply. Annual pay may vary based on FTE status.

The Bondoc Laboratory is seeking a highly motivated and experienced Research Assistant to support research focused on pediatric liver cancer, liver development, stem cell biology, and organoid models. The successful candidate will independently perform complex laboratory experiments, support animal studies, analyze data, and contribute to collaborative research projects.

Representative Responsibilities

• Research Design, perform, troubleshoot, and optimize laboratory experiments.
• Execute and adapt research protocols to meet project objectives.
• Analyze and interpret experimental data.
• Maintain accurate laboratory records and documentation.
• Contribute to publications, presentations, and other research activities.

Laboratory Techniques

• Perform mammalian cell culture, primary cell culture, and human iPSC culture.
• Conduct molecular biology assays including PCR, qPCR, and Western blotting.
• Perform histology, immunohistochemistry, immunofluorescence, and microscopy-based analyses.
• Prepare and process samples for flow cytometry and related applications.
• Assist with development, optimization, and implementation of new laboratory methods.

Animal Studies

• Maintain research mouse colonies.
• Perform animal handling, monitoring, tissue collection, and surgical procedures.
• Support in vivo studies and associated data collection.

Laboratory Operations

• Maintain laboratory equipment, supplies, and inventory.
• Troubleshoot technical and equipment issues.
• Ensure compliance with institutional, safety, biosafety, and animal care requirements.
• Support laboratory organization and day-to-day operations.

Training and Collaboration

• Train and mentor students, trainees, and junior staff.
• Serve as a technical resource within the laboratory.
• Contribute to a collaborative and productive research environment.

Preferred Experience

• Human iPSC culture and differentiation
• Organoid culture systems
• Flow cytometry and cell sorting
• Histology and immunostaining
• Confocal microscopy
• qPCR and Western blotting
• Mouse handling, colony management, and survival surgery

JOB QUALIFICATIONS

Bachelor's degree in a related field 3 years of experience in related job discipline OR Master's degree in a related field and 2 years of experience

About Us

At Cincinnati Children's, we come to work with one goal: to make children’s health better. We believe in a holistic team approach, both in caring for patients and their families, and in advancing science and discovery. We strive to do better and find energy and inspiration in our shared purpose. If you want to be the best you can be, you can do it at Cincinnati Children's.

Cincinnati Children's is: Recognized by U.S. News & World Report as a top 10 best Children's Hospitals in the nation for more than 15 years Consistently among the top 3 Children's Hospitals for National Institutes of Health (NIH) Funding Recognized as one of America's Best Large Employers (2025), America's Best Employers for New Grads (2025) One of the nation's America's Most Innovative Companies as noted by Fortune Consistently certified as great place to work A Leading Disability Employer as noted by the National Organization on Disability Magnet® designated for the fourth consecutive time by the American Nurses Credentialing Center (ANCC) We Embrace Innovation-Together. We believe in empowering our teams with the tools that help us work smarter and care better. That's why we support the responsible use of artificial intelligence. By encouraging innovation, we're creating space for new ideas, better outcomes, and a stronger future—for all of us. Comprehensive job description provided upon request.

Cincinnati Children's is proud to be an Equal Opportunity Employer committed to creating an environment of dignity and respect for all our employees, patients, and families. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, age, genetic information, national origin, sexual orientation, gender identity, disability or protected veteran status.

Cincinnati Children's is proud to be an Equal Opportunity Employer committed to creating an environment of dignity and respect for all our employees, patients, and families. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, age, genetic information, national origin, sexual orientation, gender identity, disability or protected veteran status.