Research Assistant IV - Pediatric Surgery - Bondoc Lab

CINCINNATI CHILDREN'S HOSPITAL MEDICAL CENTER; Orleans (MA); On-site; USD 58,000 - 75,000; Full time

Job summary

Cincinnati Children’s Hospital Medical Center is seeking a Research Assistant to support the Bondoc Laboratory's work on pediatric liver cancer, liver development, stem cell biology, and organoid models. You will perform complex experiments, support animal studies, analyze data, and collaborate on publications.

The role involves mammalian cell culture, molecular biology assays, histology, and in vivo work. A Bachelor’s or Master’s with experience is required.

Qualifications

• Bachelor’s degree in a related field.
• 3 years of experience in related job discipline OR Master’s degree in a related field and 2 years of experience

Responsibilities

• Design, perform, troubleshoot, and optimize laboratory experiments.
• Execute and adapt research protocols to meet project objectives.
• Analyze and interpret experimental data.
• Maintain accurate laboratory records and documentation.
• Contribute to publications, presentations, and other research activities.
• Maintain research mouse colonies.
• Perform animal handling, monitoring, tissue collection, and surgical procedures.
• Support in vivo studies and data collection.
• Maintain laboratory equipment, supplies, and inventory.
• Troubleshoot technical and equipment issues.
• Ensure compliance with institutional, safety, biosafety, and animal care requirements.
• Train and mentor students, trainees, and junior staff.
• Serve as a technical resource within the laboratory.
• Contribute to a collaborative and productive research environment.

Skills

Human iPSC culture

Organoid culture systems

Mammalian cell culture

Histology and immunostaining

Confocal microscopy

qPCR and Western blotting

Mouse handling and survival surgery

Education

Bachelor’s degree in a related field

Master’s degree in a related field with 2 years of experience

Tools

Flow cytometry

Cell sorting

Job description

Primary Location

Burnet Campus

Department

Pediatric Surgery

Shift

Day (United States of America)

Schedule

Full time

Weekly Hours

40

FTE

1

Employee Status

Regular

*Expected Starting Pay Range

$58,406.40 - $74,588.80

The Bondoc Laboratory is seeking a highly motivated and experienced Research Assistant to support research focused on pediatric liver cancer, liver development, stem cell biology, and organoid models. The successful candidate will independently perform complex laboratory experiments, support animal studies, analyze data, and contribute to collaborative research projects.

Representative Responsibilities

Research

Design, perform, troubleshoot, and optimize laboratory experiments.

Execute and adapt research protocols to meet project objectives.

Analyze and interpret experimental data.

Maintain accurate laboratory records and documentation.

Contribute to publications, presentations, and other research activities.

Laboratory Techniques

Perform mammalian cell culture, primary cell culture, and human iPSC culture.

Conduct molecular biology assays including PCR, qPCR, and Western blotting.

Perform histology, immunohistochemistry, immunofluorescence, and microscopy-based analyses.

Prepare and process samples for flow cytometry and related applications.

Assist with development, optimization, and implementation of new laboratory methods.

Animal Studies

Maintain research mouse colonies.

Perform animal handling, monitoring, tissue collection, and surgical procedures.

Support in vivo studies and associated data collection.

Laboratory Operations

Maintain laboratory equipment, supplies, and inventory.

Troubleshoot technical and equipment issues.

Ensure compliance with institutional, safety, biosafety, and animal care requirements.

Support laboratory organization and day-to-day operations.

Training and Collaboration

Train and mentor students, trainees, and junior staff.

Serve as a technical resource within the laboratory.

Contribute to a collaborative and productive research environment.

Preferred Experience

Human iPSC culture and differentiation

Organoid culture systems

Flow cytometry and cell sorting

Histology and immunostaining

Confocal microscopy

qPCR and Western blotting

Mouse handling, colony management, and survival surgery

JOB QUALIFICATIONS

• Bachelor’s degree in a related field
• 3 years of experience in related job discipline OR Master’s degree in a related field and 2 years of experience

About Us

At Cincinnati Children’s, we come to work with one goal: to make children’s health better. We believe in a holistic team approach, both in caring for patients and their families, and in advancing science and discovery. We strive to do better and find energy and inspiration in our shared purpose. If you want to be the best you can be, you can do it at Cincinnati Children’s.

Cincinnati Children’s is:

• Recognized by U.S. News & World Report as a top 10** **best Children’s Hospitals in the nation for more than 15 years

• Consistently among the top 3 Children’s Hospitals for National Institutes of Health (NIH)Funding

• Recognized as one of America’s Best Large Employers (2025), America’s Best Employers for New Grads (2025)

• One of the nation’sAmerica’s Most Innovative Companiesas noted by Fortune

• Consistently certified as great place to work

• A Leading Disability Employeras noted by theNational Organization on Disability

• Magnet® designated for the fourth consecutive time by theAmerican Nurses Credentialing Center (ANCC)

We Embrace Innovation—Together. We believe in empowering our teams with the tools that help us work smarter and care better. That’s why we support the responsible use of artificial intelligence. By encouraging innovation, we’re creating space for new ideas, better outcomes, and a stronger future—for all of us.

Comprehensive job description provided upon request.

Cincinnati Children’s is proud to be an Equal Opportunity Employer committed to creating an environment of dignity and respect for all our employees, patients, and families. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, age, genetic information, national origin, sexual orientation, gender identity, disability or protected veteran status. EEO/Veteran/Disability