Research Assistant II - Molecular Genetics & Regeneration

Marine Biological Laboratory; Falmouth (MA); On-site; USD 42,000 - 64,000; Full time

Job summary

The Rosenthal lab at the Marine Biological Laboratory (MBL) in Falmouth, MA has an opening for a full-time Research Assistant II (up to 40 hrs per week). The position is shared between two projects, split 50/50 between Dr. Rosenthal and Dr.

Diaz Quiroz, with a two-year initial appointment and potential extension based on performance. This role offers training across molecular, genetic and histological methods in emerging model organisms including RNA editing regulation and limb regeneration.

Qualifications

• Applicants must have a BS degree in biology or equivalent and prior experience in a research lab.
• Significant experience in molecular biology, histology or animal husbandry is encouraged.

Responsibilities

• Support genetic studies on squid (Euprymna berryi) and amphipods (Parhyale hawaiiensis) by generating CRISPR knockouts.
• Perform embryo microinjection with CRISPR-Cas9 and other reagents to produce genetically modified animals.
• Genotype and maintain injected animals to establish defined lines.
• Characterize the role of neuropeptides during limb regeneration in axolotls and mice; process and section tissues.
• Carry out immunostaining and/or in situ hybridization on tissue sections to examine gene expression in regenerating tissues.

Skills

Genetic studies

Lab maintenance

Record-keeping

Genotyping

Team collaboration

Education

BS in biology

Tools

CRISPR-Cas9

Embryo microinjection

PCR

Cryosectioning

Immunostaining

In situ hybridization

Job description

The Rosenthal lab at the Marine Biological Laboratory (MBL) in Falmouth, MA has an opening for a full-time Research Assistant II (up to 40 hrs per week). The position is shared between two projects, split 50/50 between Dr. Rosenthal and Dr.

Diaz Quiroz, with a two-year initial appointment and potential extension based on performance. This role offers training across molecular, genetic and histological methods in emerging model organisms including RNA editing regulation and limb regeneration.