Research Assistant

Harvarduniversity; Boston (MA); On-site; USD 50,000 - 70,000; Full time

Benefits offered by this job

Generous paid time off

Medical, dental, and vision insurance

Retirement plans with university terms

Commuter benefits

Tuition assistance and reimbursement

Job summary

The Yellen lab at Harvard Medical School Neurobiology is seeking a research technician to support studies of neuronal metabolism and its interaction with activity. You will manage a mouse colony, perform molecular biology and tissue culture, and assist with biosensor development in a dynamic lab environment.

Under close supervision, you will learn and apply techniques such as genotyping, stereotactic injections, plasmid work, and imaging workflows.

Qualifications

• One-year related experience or relevant coursework may count.
• College background or equivalent work experience, preferably in a related discipline.

Responsibilities

• Manage a moderate size mouse colony, including genotyping and mouse surgery (stereotactic injections).
• Perform molecular biology procedures including plasmid DNA prep, PCR, subcloning, biochemical assays, bacterial expression of fluorescent proteins, and fluorescence analysis.
• Perform a small amount of tissue culture (cell line maintenance and transfection).

Skills

Mouse handling

Molecular biology

PCR

Subcloning

Sterile technique

Education

College degree or equivalent

Biology background

Tools

Stereotactic surgery

Job description

The Yellen lab at Harvard Medical School Neurobiology is looking for a research technician. Our research is focused on understanding neuronal metabolism and how it is influenced by - and influences - neuronal activity and excitability. The lab develops tools for studying neural metabolism, including engineered proteins that function as fluorescent biosensors of metabolism, and we study the dynamics of metabolism in mouse brain using both ex vivo and in vivo imaging methods. We also work to learn the role of altered metabolism in preventing epileptic seizures, inspired by the ability of ketogenic diets to treat human epilepsy.

The successful candidate will work under close supervision by Dr Yellen and others in the lab to:

1. manage a moderate size mouse colony, including genotyping and performing mouse surgery (stereotactic injection of viral expression vectors);
2. perform molecular biology procedures including plasmid DNA preparation, PCR, subcloning, biochemical assays, bacterial expression of fluorescent proteins, and fluorescence analysis; and
3. perform a small amount of tissue culture (cell line maintenance and transfection).

There may also be the opportunity to do semi-independent projects on fluorescent biosensor development and/or biosensor imaging. The successful candidate should be well-organized, motivated, and a responsible team member.

Basic Qualifications:

• At least one-year related work experience (relevant course work may count towards experience).
• College background or equivalent work experience, preferably in a related discipline.

Additional Qualifications and Skills:

The candidate should have a general education in molecular biology and should be familiar with the basic concepts of plasmids, expression vectors, and subcloning, as well as hands-on experience with good laboratory practices and sterile technique.

Some experience with mice is preferred.

Detailed guidance and training will be provided for each of the specific procedures used. All of the work requires attention to detail, and some of it requires creative problem solving.

In general, the successful candidate is someone who always has their "head in the game."

Term:

This is a one-year term position from the date of hire, with the possibility of extension, contingent upon work performance and continued funding to support the position.

Standard Hours/Schedule:

35 hrs. per week | Monday - Friday | 9:00 am - 5:00 pm

Visa Sponsorship Information:

Harvard University is unable to provide visa sponsorship for this position.

Pre-Employment Screening:

Identity, Criminal, Credit, Pre-Placement Medical Evaluation

Work Format Details

This position has been determined by school or unit leaders that all duties and responsibilities must be performed at a Harvard or Harvard-designated location. Certain visa types may limit work location. Individuals must meet work location sponsorship requirements prior to employment.

Salary Grade and Ranges

This position is salary grade level 052. Please visit Harvard's Salary Ranges to view the corresponding salary range and related information.

Benefits

• Generous paid time off including parental leave
• Medical, dental, and vision health insurance coverage starting on day one
• Retirement plans with university contributions
• Wellbeing and mental health resources
• Support for families and caregivers
• Professional development opportunities including tuition assistance and reimbursement
• Commuter benefits, discounts and campus perks
• Learn more about these and additional benefits on our Benefits & Wellbeing Page .

EEO/Non-Discrimination Commitment Statement

Harvard University is committed to equal opportunity and non-discrimination . We seek talent from all parts of society and the world, and we strive to ensure everyone at Harvard thrives. Our differences help our community advance Harvard's academic purposes.

Harvard has an equal employment opportunity policy that outlines our commitment to prohibiting discrimination on the basis of race, ethnicity, color, national origin, sex, sexual orientation, gender identity, veteran status, religion, disability, or any other characteristic protected by law or identified in the university's non-discrimination policy . Harvard's equal employment opportunity policy and non-discrimination policy help all community members participate fully in work and campus life free from harassment and discrimination.