Post-doctoral position

scBiomarkers UTechS - Institut Pasteur; Wall Lake (IA); On-site; USD 48,000 - 62,000; Full time

Job summary

The Institut Pasteur invites applications for a post-doctoral fellowship funded by ANR in microbiome studies. The successful candidate will join the Biology and Genetics of the Bacterial Cell Wall Unit led by Ivo G.

Boneca to study Helicobacter pylori and peptidoglycan fragment release using organoids, gut-on-chip and in vivo models. Candidates should have strong microbiology and biochemistry expertise, plus experience in mass spectrometry, cellular/animal models and confocal microscopy.

Qualifications

• Strong microbiology and organoid experience required.
• Experience with imaging techniques such as confocal microscopy is preferred.
• Interest in microbiome and peptidoglycan biology advantageous.

Responsibilities

• Study Helicobacter pylori mechanisms of peptidoglycan release in organoid and in vivo models.
• Apply organoid, gut-on-chip and imaging approaches to immunomodulation by peptidoglycan fragments.
• Collaborate with international teams on project milestones.

Skills

Microbiology expertise

Organoid experience

Mass spectrometry

Confocal/immunofluorescent microscopy

Tools

LC-MS/MS

Job description

Post-doctoral position: We have a fellowship funded by the ANR for a post-doctoral fellow specialized in microbiome studies at the Institut Pasteur.

The selected candidate will be working at the Biology and Genetics of the Bacterial Cell Wall Unit headed by Ivo G. Boneca. This is a collaborative project with the teams of Raquel Mejias-Luque of the Technical University of Munich and of Richard Ferrero of the Monash University.

The project builds on our previous work on the dissemination of peptidoglycan fragments across the body from the gut.

We are looking for somebody that has highly transversal expertise in biochemistry of peptidoglycan, mass spectrometry, cellular and animal models, and in confocal/immunofluorescent microscopy. The postdoc will study the model organism Helicobacter pylori and its mechanisms of release of immune-stimulatory peptidoglycan fragments to shape the host immune system using in vitro organoids, gut-on-chip and in vivo models.

The successful candidate should have a strong expertise in microbiology and organoids. Expertise with animal models and/or by LC-MSMS analysis would be a plus.

Viala J, C Chaput, IG Boneca, A Cardona, SE Girardin, AP Moran, R Athman, S Mémet, M Huerre, AJ Coyle, PS DiStefano, PJ Sansonetti, A Labigne, J Bertin, DJ Philpott, RL Ferrero. 2004. Nod1 responds to peptidoglycan delivered by the Helicobacter pylori cag pathogenicity island. Nat Immunol. 5(11):1166-1174. doi: 10.1038/ni1131.

Grubman A, M Kaparakis, J Viala, C Allison, L Badea, A Karrar, IG Boneca, L Le Bourhis, S Reeve, IA Smith, EL Hartland, DJ Philpott, RL Ferrero. 2010. The innate immune molecule, NOD1, regulates direct killing of Helicobacter pylori by antimicrobial peptides. Cell Microbiol. 12(5):626-639. doi: 10.1111/j.1462-5822.2009.01421.x.

Irving A, H Mimuro, T Kufer, C Lo, R Wheeler, L Turner, BJ Thomas, C Malosse, MP Gantier, LN Casillas, BJ Votta, J Bertin, IG Boneca, C Sasakaw, DJ Philpott, RL Ferrero, M Kaparakis-Liaskos. 2014. NOD1 interacts with peptidoglycan and RIP2 on early endosomes to promote RIP2-dependent autophagy and inflammatory signaling. Cell Host Microbe. 15(5):623-635. doi: 10.1016/j.chom.2014.04.001.

Dias Bastos PA, R Wheeler, IG Boneca. 2021. Uptake, recognition and responses to peptidoglycan in the mammalian host. FEMS Microbiol Rev. 45(1):fuaa044. https://doi.org/10.1093/femsre/fuaa044

Wheeler R, PA Dias Bastos, O Disson, A Rifflet, I Galbanyi, J Spielbauer, M Berard, M Lecuit, IG Boneca. 2022. Microbiota-induced active translocation of peptidoglycan across the intestinal barrier dictates its within-host dissemination. Proc Natl Acad Sci U S A. 120(4):e2209936120. doi: 10.1073/pnas.2209936120.

Contact

Ivo G. Boneca [e-mail]