Optogenetic Protein Engineer – Research Scientist

University of Washington; Seattle (WA); Sur place; USD 56 000 - 58 000; Plein temps

Résumé du poste

The University of Washington in Seattle invites applications for a research assistant to support engineering and characterization of optogenetically controlled RNA polymerases and related nucleic-acid writing systems. The role focuses on cloning, protein production, and cell-free assays to generate sponsor milestones and publications.

Candidates should hold a Bachelor's in a relevant field and have hands-on lab experience in molecular biology.

Qualifications

• Bachelor's degree in Biochemistry, molecular biology, bioengineering, chem biol, or related field and one year of relevant experience laboratory research.
• Equivalent education and/or experience may substitute for minimum qualifications except when there are legal requirements, such as a license, certification, and/or registration.
• Molecular cloning: PCR, Gibson/Golden Gate assembly, mutagenesis, bacterial transformation, plasmid preparation, and sequence verification.
• Protein production: bacterial expression, affinity purification, chromatography/FPLC workflows, and protein QC.
• Biochemical assays: experience with enzyme assays, transcription assays, fluorescence-based readouts, or related in vitro functional measurements.
• research assistantships/internships acceptable, strong hands-on

Responsabilités

• Molecular cloning and construct generation – 25%
• Protein expression, purification, and QC – 20%
• In vitro functional assay development and execution – 25%
• Optogenetic characterization and readout integration – 15%
• Sequencing sample preparation and data/QC handoff – 10%
• Project support, documentation, and collaboration – 5%

Connaissances

Molecular cloning

Protein expression

Biochemical assays

Optogenetics

Sequencing

Documentation

Formation

Bachelor's degree in Biochemistry, molecular biology, bioengineering, chem biol, or related field

Outils

PCR

Gibson/Golden Gate

Description du poste

The University of Washington in Seattle invites applications for a research assistant to support engineering and characterization of optogenetically controlled RNA polymerases and related nucleic-acid writing systems. The role focuses on cloning, protein production, and cell-free assays to generate sponsor milestones and publications.

Candidates should hold a Bachelor's in a relevant field and have hands-on lab experience in molecular biology.