Laboratory Technician II

University of Florida; Gainesville (FL); On-site; USD 26,000 - 30,000; Full time

Job summary

University of Florida is seeking a Laboratory Technician II to support animal studies, histology, and molecular biology workflows within the College of Medicine. The role involves treating animals, conducting imaging support, and performing biotechnical assays, while ensuring regulatory compliance and data integrity.

The candidate will train and supervise graduate and undergraduate students, maintain records meticulously, and contribute to translational cancer research initiatives.

Qualifications

• Associate's degree in an appropriate area and two years of related experience; or a high school diploma or equivalent and four years of related experience.
• Appropriate college course work or vocational/technical training may substitute at an equivalent rate for the required experience.

Responsibilities

• Treat animals with pharmacological agents or immunotherapy via i.p., i.m., i.v., or intracranial injection.
• Assist with MRI, CT, and MPI imaging techniques in mice.
• Participate in regulatory management (IACUCs).
• Collect organs/tissues and prepare for histopathology; stain and analyze tissues.
• Perform molecular biology tasks including RNA/DNA isolation, real-time PCR, and genotyping of transgenic mice.
• Conduct protein analysis and western blots; perform flow cytometry and multi-parameter panels.

Skills

Rodent handling

Mice experiments

Record keeping

Education

Associate's degree

High school diploma + 4 years related experience

Tools

Immunohistochemistry

FACS

RT-PCR

Genotyping

Western blotting

Microscopy

Job description

Classification Minimum Requirements

Associate's degree in an appropriate area and two years of related experience; or a high school diploma or equivalent and four years of related experience. Appropriate college course work or vocational/technical training may substitute at an equivalent rate for the required experience.

Classification Title

Laboratory Technician II

Classification Minimum Requirements

Associate's degree in an appropriate area and two years of related experience; or a high school diploma or equivalent and four years of related experience. Appropriate college course work or vocational/technical training may substitute at an equivalent rate for the required experience.

Job Description

Animal Studies: Participating in treating animals with pharmacological agents or immunotherapy either through i.p., i.m., i.v., or intracranial injection. Other duties with the mice include assisting with MRI, CT, and MPI imaging techniques. Assist with experimental approaches using magnetic nanoparticles. Plays a key role in regulatory management (IACUCs).

Histological Work/Analysis: Collecting organs and other tissues and preparing for histopathology following the preparation of slides, staining with standard histology stains such as H&E, as well as with immunohistochemistry and immunofluorescence will be done. Conduct image capture with fluorescent microscope, confocal microscope, multiphoton confocal microscope and analysis of tissue following intervention studies.

Molecular Techniques and Analysis: Molecular cloning, Plasmid purification, Primer design, RNA and DNA isolation, Isolating RNA and performing real-time PCR, and PCR genotyping of transgenic mouse lines; immunoassays to gene therapy vectors and transgenes, including ELISAs. This may include expansion/maintenance of cell lines and analysis of transfection. Performing protein analysis by isolating protein of interest from cell or tissue lysates and performing/analyzing western blots. Perform multiparameter flow cytometry and develop proficiency setting multicolor flow panels.

Regulatory Compliance: Ensures the laboratory is compliant with governing rules and regulations. Coordinate all IACUC and IRB (exempt) submissions/renewals. Ensure all EH&S standards are met. Train new laboratory members to ensure compliance standards are met.

Training: Train and supervise graduate and undergraduate students performing diagnostic procedures related to immune therapy experiments.

Any other duties, as needed, to fulfill the mission and to abide by the values of the College of Medicine. This will include performing tasks and experiments as directed by the Principal Investigator

Expected Salary

$19.23 - $22.00/hour

Required Qualifications

Associate's degree in an appropriate area and two years of related experience; or a high school diploma or equivalent and four years of related experience. Appropriate college course work or vocational/technical training may substitute at an equivalent rate for the required experience.

Preferred

• Animal colony management and experience handling rodents (highly preferred).
• Experience administering treatments i.v., i.p., i.m., and/or intracranially to rodents (highly preferred).
• Experience maintaining cell lines, performing plasmid transfections/viral infections, immunohistochemistry, FACS, rt-PCR, genotyping, immunohistochemistry, western blotting, microscopy
• Advanced degree in related scientific field.
• Working knowledge of basic laboratory techniques.
• Must be extremely knowledgeable in laboratory procedures and must be meticulous in recording, tracking & reporting of data collected.
• Strong interest in translational cancer research

Health Assessment Required: Yes