Laboratory Assistant I

NCIRE; San Francisco (CA); On-site; USD 100,000 - 125,000; Full time

Job summary

A leading research lab in San Francisco is offering an entry-level research assistant position. This role involves laboratory experience in studies on exosomes and their roles in cardiometabolic diseases, providing an excellent opportunity for candidates aiming for health professional programs.

Qualifications

• Experience with RNA/DNA and protein isolation methods.
• Experience with biochemical analysis.
• Ability to work collaboratively and assist in training junior staff.

Responsibilities

• Assist PI and senior staff with routine laboratory upkeep.
• Assist with transgenic mouse breeding and PCR genotyping.
• Assist with exosome production and study in cell signaling experiments.

Skills

Attention to detail

Communication

Interpersonal skills

Organizational skills

Education

BS in molecular and cellular biology

BS in biochemistry

Final year of BS program

Tools

Windows Excel

Adobe Photoshop

Job description

Job Details

Description

The Atherosclerosis Research Lab within the UCSF Department of Surgery is offering an entry-level research assistant position. This opportunity is intended for a senior or recent graduate interested in gaining laboratory experience in preparation for entry into a health professional program.

Our laboratory has pioneered the study of extracellular vesicles and exosomes produced by macrophages as modulators of cardiometabolic diseases including diabetes, obesity, and atherosclerosis. Ongoing projects aim to study the role of diabetes and the APOE4 gene in altering macrophage-exosome biology and their impact on accelerating atherosclerosis, cardiac dysfunction, and neurodegeneration in mouse models. We are also developing new methods to produce exosomes that could serve as next-generation biologic medicines to control cardiovascular disease and diabetes.

The successful applicant will assist senior lab staff with studies involving techniques such as exosome production and isolation, western blot, RNA/DNA extraction, PCR, flow cytometry, immunohistochemistry, ultracentrifugation, cell culture, mouse surgery, and husbandry.

Our trainees typically dedicate 2-4 years to our program before moving on to health professional programs, with multiple publications credited to their names. Examples of recent publications can be found online:

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Essential Functions

• Assist PI and senior staff with routine laboratory upkeep.
• Assist with transgenic mouse breeding and PCR genotyping.
• Assist with gel electrophoresis preparation and Western blotting.
• Assist with exosome production and isolation from cultured cells and biofluids, and their study in cell signaling experiments.
• Assist with harvesting mouse tissues and plasma, and isolating hematopoietic cells and lipoproteins using centrifugation and flow cytometry.

Job Requirements

• BS in molecular and cellular biology, biochemistry, or in the final year of a BS program.
• Experience with RNA/DNA and protein isolation methods and biochemical analysis.
• Ability to work collaboratively and assist in training junior staff.
• Strong communication, interpersonal, and organizational skills.
• Accuracy and attention to detail.
• Computer literacy, especially with Windows Excel and Adobe Photoshop for data analysis and visualization.

The salary range for this position is $18.48 - $21.42 per hour. The actual offer will consider various factors including geographic location, experience, skills, education, and internal equity. Additional compensation such as bonuses may also be available.

NCIRE is an Equal Opportunity Employer. This position requires access to US Government research under the Veterans Administration. Qualified US citizens will be prioritized as per VA regulations. Consideration of non-citizens is possible only if no qualified US citizens apply.