Lab Technician I - Laboratory

Genesis Global Group; Pennsylvania; In loco; USD 45.000 - 65.000; Tempo pieno

Vantaggi offerti da questo lavoro

Competitive Salary

Medical, Dental & Vision Insurance

401(k) Plan

Paid Vacation & Holiday

Descrizione del lavoro

MDL, a member of Genesis Biotechnology Group, is a CLIA-certified clinical laboratory in Pennsylvania, specializing in PCR-based infectious disease testing. We are seeking a Laboratory Technician to support specimen handling, labeling, accessioning, and molecular workflows in a fast-paced lab.

The role covers manual and robotic extractions, PCR setup, gel electrophoresis, sequencing prep, and data documentation while ensuring safety and patient privacy. Competitive benefits are offered.

Competenze

• Bachelor's degree in biology, chemistry, or a related science field.
• 9-month clinical laboratory technician training.
• Able to work in a CLIA-certified laboratory environment.

Mansioni

• Print labels and attach requisitions to specimens.
• Accession multiple specimen types and photograph all specimens.
• Identify any discrepant information and file paperwork.
• Operate and set up thermocyclers for PCR and analyze results.
• Perform manual and robotic extraction (phenol/chloroform, RNA, and DNA).
• Handle gel electrophoresis and sequencing preparatory steps.
• Maintain safety and privacy standards in the lab.

Conoscenze

Laboratory techniques

Formazione

Bachelor Degree in Biology, Chemistry, or related science field

Strumenti

Thermocycler

Pipette set

SYNGENE UV gel reader

Pippin Prep

Rotor-gene thermocycler

Bio-Rad CFX thermocycler

Descrizione del lavoro

Laboratory Technician

Medical Diagnostic Laboratories (MDL), a member of Genesis Biotechnology Group, is a CLIA certified clinical laboratory with multiple state licensing, specializing in state of the art, automated DNA based molecular analysis of a variety of chronic and infectious illnesses.

MDL specializes and performs Polymerase Chain Reaction (PCR) with a larger menu of testing available in the field of infectious disease. Our main theme of research is in the field of Gynecology, Infectious Diseases, Infectious Arthritis, Tick-borne Diseases, Mycology, and Chronic Fatigue Syndrome (CFS).

MDL is looking for a Laboratory Technician. The Laboratory Technicians responsible for printing labels and attaching requisitions to the corresponding specimens.

Essential Functions

• Print labels and attach to requisitions and the corresponding specimens
• Properly accession multiple specimen types
• Take photographs of all specimens received
• Properly identify any discrepant information
• File all paperwork in order and distribute to the appropriate QC bins
• Properly scan accessioned requisitions
• Scan 2-D barcode elution tubes used for extraction
• Properly locate and record temperatures for all necessary stations

Manual Extraction

• Perform Phenol/Chloroform manual extractions on biopsies and ticks
• Properly separate samples into their organic and inorganic layers via phenol/chloroform
• Properly perform quantitative analysis and hydrate all ticks and biopsies

Robotic Extraction

• Properly extract all samples types using a provided XG sheet as a template
• Properly set up liquid handling robotics for each extracted XG sheet with the correct volumes of all necessary reagents
• Properly dispose of all waste material when required; both hazardous and non-hazardous
• Attend to robotics to address any user interactions and/or robotic malfunctions
• Manually extract all necessary samples via commercial kits

RNA Extraction

• Perform manual RNA extractions via Qiagen kit for all necessary samples
• Perform robotic RNA extractions via VX Reagents kit for all necessary samples

PCR

• Perform manual and robotic Conventional method PCR amplification
• Properly load and start thermocycler machines
• Perform manual and robotic Real-time method PCR amplification
• Properly load and start Rotor-gene thermocycler
• Perform initial analysis on completed Real-time PCR
• Perform Acoustic Real-Time PCR method for amplification with the aid of the ATS-100 Acoustic Dispensor
• Properly load and start Bio-Rad CFX thermocycler
• Perform initial analysis on completed Acoustic Real-Time PCR

Gel Electrophoresis

• Properly identify which completed PCRs from the thermocycler require gel electrophoresis
• Properly identify when to make and/or use 2% versus 2.5% gels
• Complete blank gel template sheets to combine short PCR sheets
• Use dye to stain all necessary samples with the appropriate volume on a test by test basis
• Properly load correct agarose gel with the appropriate volume of ladder and sample; in addition properly identify the appropriate settings on which to run each gel (i.e. voltage & time)
• Properly capture a visual image of the gel with the use of a SYNGENE UV gel reader
• Be able to troubleshoot any gel box or power source errors, as well as any gel reader errors

Pyrosequencing

• Properly perform nested PCR prior to sequencing
• Properly load and run Sequencing Analysis reactions (SQA)
• Properly load and run Single Nucleotide Polymorphism reactions (SNP)
• Properly analyze all run data

Micro-Biology/Cell Culture

• Inoculate appropriate plate with swab media; for Candida species, Urinary Pathogens, GBS, Enterococcus species, and the Wound Panel; Streak for single colonies
• Properly determine specific species present, and inoculate appropriate plate with said species on a quad plate preloaded with antibiotics, or an agar plate stamped with antibiotic discs
• Properly analyze growth plate to determine antibiotic susceptibility or resistance
• For GBS Antibiotic Resistance testing, properly assemble rack and operate Anaerobic Chamber System
• Identify and pick colonies for PCR
• Perform CAMP test and analyze for zones of hemolysis

Next-Generation Sequencing

• Properly perform a series of nested PCRs for BRCA, HPV, and Cystic Fibrosis
• Properly attach samples to appropriate bead mixtures
• Properly perform beads cleaning to remove unused dNTP’s and primers within the amplified PCR product
• Properly perform gel purification with the use of the Pippin Prep machine
• Properly load and operate multiple Life Technologies Equipment
• Properly analyze all run data

Additional Responsibilities

• Performs other similar or related duties as required or assigned.
• Assures activities are conducted in a safe manner and materials are handled according to safety guidelines.
• Assures customer privacy is maintained at all times.
• Participates as a team member and is accountable for own work responsibilities.
• Assures information is delivered in a manner that is supportive, timely and understandable.
• Initiative is demonstrated to proactively diagnose and resolve problems.
• Productive work habits are consistently displayed.
• Accuracy and quality are maintained for all activities.

Job Qualifications

General Knowledge, Skills, and Abilities (KSA’s) required

• 9-month Clinical Laboratory Technician training

Physical Demands

• Ability to sit, stand, walk and perform repetitive motion (manual pipette)
• Proficient visual and audio skills

Workplace Conditions

• Laboratory setting
• Use of personal protective equipment may be necessary
• May be exposed to chemicals, blood/body fluids

Education and Certifications

• Bachelor Degree in Biology, Chemistry, or related science field

We Offer

• Competitive Salary
• Medical, Dental & Vision Insurance
• Short and Long Term Disability
• Life Insurance
• Dependent Care Flex Spending Account
• Voluntary Policies (Accident, Hospital Indemnity, Critical Illness & Supplemental STD)
• Identity Theft Protection
• 401(k) Plan
• Paid Vacation & Holiday
• Business Casual Environment

Visit us at https://www.genesisglobalgrp.com/

Genesis Biotechnology Group is a member of Genesis Global Group, an equal opportunity employer.