Junior Specialist

UC San Francisco Academic; San Francisco (CA); In loco; USD 57.000 - 61.000; Tempo pieno

Descrizione del lavoro

UCSF Department of Cellular and Molecular Pharmacology seeks a Junior Specialist. Typical duties include mouse colony maintenance and genotyping, transcardial perfusions and tissue harvesting, cryostat brain sectioning, biochemistry work (Western blot, ELISA) and cell culture maintenance using sterile technique.

Required: Baccalaureate degree (or equivalent) or at least four years of research experience. Preferred: lab experience in biochemistry or molecular biology, protein

Competenze

• Baccalaureate degree (or equivalent) or at least four years of research experience.
• Prior laboratory experience in biochemistry, molecular biology, or related field preferred.
• Experience with protein expression, purification, and biochemical assays is a plus.

Mansioni

• Mouse colony maintenance and genotyping (breeding, weaning, tagging, PCR).
• Transcardial perfusions and tissue harvesting.
• Cryostat brain sectioning.
• Biochemistry work including Western blotting and ELISA.
• Cell culture maintenance using sterile technique.

Conoscenze

Mammalian cell culture

Biochemistry techniques

Genotyping (PCR)

Western blotting

ELISA

Histology basics

Lab skills

Formazione

Baccalaureate degree (or equivalent)

Descrizione del lavoro

Junior Specialist
Department of Cellular and Molecular Pharmacology
University of California, San Francisco

The Department of Cellular and Molecular Pharmacology is now recruiting a Junior Specialist. Some of the position duties include:

• Mouse colony maintenance and genotyping (breeding, weaning, tagging, and PCR)
• Transcardial perfusions and tissue harvesting
• Cryostat brain sectioning
• Biochemistry work, including Western blotting , ELISA and isolating and purifying circulating tagged proteins from mouse plasma
• Cell culture maintenance using sterile technique

Required Qualifications

• Junior Specialist - Baccalaureate degree (or equivalent degree) or at least four years of research experience

Preferred Qualifications

• Some prior laboratory research experience and/or exposure in biochemistry, molecular biology, or a related field, including experience with protein expression, purification, and biochemical assays.
• Experience with mammalian cell culture using sterile technique and Western blotting.
• Experience with, or familiarity with, histological techniques and tissue processing.

Baccalaureate degree (or equivalent degree) or at least four years of research experience

See Table 24B for the salary range for this position. A reasonable estimate for this position is $57,000-$60,700.