iPSC Neuron Research Associate

Quiver Bioscience; Cambridge (MA); On-site; USD 75,000 - 110,000; Full time

Job summary

Quiver Bioscience seeks a biology-focused professional to execute and troubleshoot production and QC of neuronal cultures. You will maintain iPS cell lines and differentiate them into excitatory/inhibitory neurons, plate in 96/384-well formats, and perform RNA/DNA prep along with qPCR readouts.

You will also assess protein expression via ICC and immunoblotting, assist with lab operations, and present progress in internal meetings.

Qualifications

• Master’s degree in biology, cell biology, biotechnology or related field.
• At least two years’ experience with mammalian cell culture and iPS-derived neurons.
• Experience with DNA/RNA/protein prep and readouts (PCR, qPCR, ICC, Western blot).

Responsibilities

• Execute and troubleshoot production and QC of neuronal cultures for Quiver’s multiple customer-based and internal R&D projects.
• Maintain and characterize multiple human iPS cell lines.
• Use transcriptional programming and directed differentiation strategies to produce various types of high-quality human iPS cell-derived neurons.
• Plate and maintain cultures of human iPS cell-derived neurons in 96-well and 384-well assay formats.
• Carry out RNA and DNA preparations from human cell lines and cultured neurons.
• Perform candidate gene expression analysis using quantitative PCR assays.
• Assess protein expression using immunocytochemistry (ICC) and immunoblotting assays.
• Assist with cell culture lab operations, including inventory, purchasing of lab reagents and supplies, and aliquoting of recombinant protein and lentiviral particle stocks.
• Communicate work progress via technical reports, PowerPoint presentations, and seminars at internal meetings.

Skills

Mammalian cell culture

iPS cell-derived neurons

DNA/RNA/Protein preparations

PCR

qPCR

Immunocytochemistry (ICC)

Immunoblotting

Scientific communication

Education

Master’s degree in biology or closely related field

Tools

96/384-well assay formats

Job description

Quiver Bioscience seeks a biology-focused professional to execute and troubleshoot production and QC of neuronal cultures. You will maintain iPS cell lines and differentiate them into excitatory/inhibitory neurons, plate in 96/384-well formats, and perform RNA/DNA prep along with qPCR readouts.

You will also assess protein expression via ICC and immunoblotting, assist with lab operations, and present progress in internal meetings.