CryoEM Staff Scientist

Howard Hughes Medical Institute (HHMI); Ashburn (VA); On-site; USD 110,000 - 165,000; Full time

Benefits offered by this job

On-site childcare

Campus housing

Shuttle service to DC metro area

Job summary

HHMI's CryoEM Shared Resource at Janelia Research Campus seeks a scientist who wants their scientific judgment to shape data, not just operate equipment. You’ll collect high-throughput data with Titan Krios, Aquilos2, and Hydra cryo FIBSEM and collaborate with users across the HHMI community.

You’ll lead cryoEM aspects of research collaborations, develop, implement, and test new protocols for sample preparation, data collection, and image processing, and train users and staff to share expertise

Qualifications

• PhD in a related field and ability to view experiments from a collaborator's perspective.
• Proficiency with Linux, bash, and Python to troubleshoot issues.
• Ability to manage multiple simultaneous user projects.
• Experience or aptitude for teaching and training others in cryoEM methods.

Responsibilities

• Run the microscopes end to end and troubleshoot hardware/software with peers.
• Lead cryoEM portions of collaboration projects from planning to results review.
• Develop, implement, and test new protocols for sample prep, data collection, and image processing.
• Keep the facility current by adopting new cryoEM developments.
• Train users and staff on cryoEM methods and equipment.
• Handle samples, cryogen dewars, dry shippers, and data transfer properly.

Skills

Linux

bash scripting

Python

Education

PhD in related field

Tools

Titan Krios

Aquilos2

Hydra cryo FIBSEM

Job description

This is a role for someone who wants their scientific judgment to show up in other people's data, not just their own.

The CryoEM Shared Resource at Janelia Research Campus runs four highly configured Titan Krios microscopes, an Aquilos2 and a Hydra cryo FIBSEM, screening electron microscopes, cryo light microscopes, and a full grid-preparation setup, serving structural and cell biologists across the entire HHMI community.

You’ll collect high-throughput data for users, take a leading role in the cryoEM side of collaboration projects, and build new methods and protocols when the existing ones fall short.

You’ll work closely with users and collaborators throughout: planning experiments together, reviewing what the data shows, and figuring out the next step as a peer, not just an instrument operator.

What you will actually do

• Run the microscopes, end to end. Operate and maintain equipment from sample-preparation systems to the Krios microscopes, and troubleshoot hardware and software issues directly with peers and vendor engineers.

• Own pieces of collaboration projects. Take a leading role in the cryoEM portion of research collaborations, from planning experiments through reviewing results and proposing next steps.

• Build new protocols and methods. Develop, implement, and test new protocols for sample preparation, data collection, and image processing, and fold what works into the facility's standard workflow.

• Keep the facility current. Track new developments in cryoEM and actively bring useful techniques back into the workflow rather than waiting for someone else to.

• Teach it forward. Train users and new staff on cryoEM methods and equipment so the facility's expertise doesn't live in one person's head.

• Handle the physical realities. Manage samples, cryogen dewars, and dry shippers correctly. A mishandled sample is data nobody gets back. Manage and transfer data properly.

What we are looking for

• A scientist first. You hold a PhD in a related field and think about an experiment the way a collaborator would, not just an instrument operator.

• Fluent in the hardware. Comfortable with Linux, bash scripting, and basic Python, enough to troubleshoot a problem rather than just report it.

• Steady inside a moving target. You can hold several concurrent user projects in your head and still make room for whatever breaks that day.

• A real teacher. You enjoy explaining a technique as much as running it, and training someone else doesn't feel like a distraction from your work.

Nice to have

• Hands-on experience with cryoEM sample preparation, data collection, or image processing.

• A background in 4D STEM, if you're coming from outside biology.

• Experience with image-processing software.

What this role is not

• Not a role you can do on scientific judgment alone. You're also running and troubleshooting sophisticated hardware every day, hands on.

• Not a role you can do on technical skill alone. You're constantly translating what a user needs into an experiment, then explaining the result back to them.

• Not a customer-service role that happens to sit next to a microscope. The service mindset matters, but so does the science and the hardware fluency behind it.

Practical details

Based on site at HHMI's Janelia Research Campus in Ashburn, Virginia. Requires a PhD in a related field. Comes with a competitive total-rewards package, including on-site childcare, campus housing, and shuttle service to and from the Washington, DC metro area.

Physical Requirements

Remaining in a normal seated or standing position for extended periods of time; reaching and grasping by extending hand(s) or arm(s); dexterity to manipulate objects with fingers, for example using a keyboard; communication skills using the spoken word; ability to see and hear within normal parameters; ability to move about workspace. The position requires mobility, including the ability to move materials weighing up to several pounds (such as a laptop computer or tablet).

Persons with disabilities may be able to perform the essential duties of this position with reasonable accommodation. Requests for reasonable accommodation will be evaluated on an individual basis.

Please Note:

This job description sets forth the job’s principal duties, responsibilities, and requirements; it should not be construed as an exhaustive statement, however. Unless they begin with the word “may,” the Essential Duties and Responsibilities described above are “essential functions” of the job, as defined by the Americans with Disabilities Act.

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