Brain Tumor Targeting Imaging Research Assistant

Brigham and Women's Hospital; Boston (MA); On-site; USD 60,174,000 - 83,097,000; Full time

Job summary

The Brigham and Women's Hospital (BWH) is recruiting an early-career Technical Research Assistant I to support a multidisciplinary program focused on rare brain tumors. You will identify and validate therapeutically actionable cell-surface proteins, with emphasis on ADC targets, using advanced tissue-imaging and spatial technologies.

You will work with CyCIF, ORION, IHC/IF, and spatial transcriptomics, collaborating with Harvard Medical School and Dana-Farber, to map targets in patient tissues

Qualifications

• Bachelor's degree in biology, biochemistry, biomedical engineering, or a related field.
• Demonstrated interest in biomedical research, cancer biology, molecular measurement, tissue imaging, or quantitative analysis.
• Prior lab experience, particularly involving microscopy, immunofluorescence, molecular or biochemical assays, or quantitative data analysis.

Responsibilities

• Develop a spatial molecular atlas of therapeutic targets across brain tumors by generating and analyzing multiplexed tissue-imaging data.
• Perform multiplexed tissue-imaging experiments (CyCIF, ORION, IF/IHC) to quantify cell-surface targets.
• Develop and qualify antibodies against targets, compare clones and optimize staining for specificity and signal.
• Design multiplex antibody panels with targets and tumor markers.
• Prepare and analyze FFPE tissues, TMAs, and patient-derived models with scarce samples.
• Quantify whole-slide images; QC, single-cell segmentation, target expression, spatial patterns.
• Integrate protein imaging with spatial transcriptomics to prioritize targets.
• Support studies comparing patient tumors with derived cell lines and xenografts.
• Help develop standardized ORION imaging assays for future trials.
• Maintain detailed experimental records and quality-control datasets.
• Troubleshoot and optimize protocols and contribute ideas for improvements.
• Prepare figures and datasets for presentations and manuscripts.
• Present work at lab meetings and discuss results and next steps.

Skills

Immunofluorescence

Microscopy

Quantitative data analysis

Image analysis

Lab protocol optimization

Education

Bachelor's degree in biology or related field

Tools

ImageJ/Fiji

Python

R

MATLAB

Job description

The Brigham and Women's Hospital (BWH) is recruiting an early-career Technical Research Assistant I to support a multidisciplinary program focused on rare brain tumors. You will identify and validate therapeutically actionable cell-surface proteins, with emphasis on ADC targets, using advanced tissue-imaging and spatial technologies.

You will work with CyCIF, ORION, IHC/IF, and spatial transcriptomics, collaborating with Harvard Medical School and Dana-Farber, to map targets in patient tissues