Biologist (52837)

GAP SOLUTIONS INC; Baltimore (MD); On-site; USD 135,000 - 145,000; Full time

Job summary

GAP Solutions Inc. is seeking an experienced Biologist for the NIH Baltimore Campus to support the Confocal Imaging Facility core of the NIA IRP.

The role focuses on providing research services and deliverables through advanced microscopy and related laboratory activities. The ideal candidate holds a Master’s degree in biology, has hands-on experience with confocal microscopy, immunohistochemistry, and electron microscopy techniques, and can operate and troubleshoot several imaging systems while

Qualifications

• Master’s degree in biology or a related field.
• Proficiency in Confocal Light Microscopy.
• Must have 4 years demonstrated experience in biological research with embedding and ultramicrotomy for electron microscopy.
• Skilled in Zeiss Zen, FIJI-ImageJ, and MS Office.
• Experience with cryo-fixation preparation techniques.
• 2–5 years using SEMs or TEMs.
• Experience with Focused Ion Beam Scanning.
• Familiarity with automated imaging systems for light or electron microscopes.
• Experience with digital imaging of biology using light microscopy.
• Familiarity with 3D reconstruction software.

Responsibilities

• Study basic principles of plant and animal life.
• Identify, classify, and study structure and function of species.
• Operate confocal microscopy and analyze images.
• Perform IHC/ICC and immunofluorescence techniques.
• Apply super-resolution microscopy concepts.
• Prepare TEM samples and perform related protocols.
• Troubleshoot and optimize lab protocols and instruments.
• Train and support other researchers in confocal microscopy.
• Manage data storage, processing, and analysis.
• Maintain organized laboratory records and supplies.

Skills

Confocal microscopy

Immunohistochemistry

Electron microscopy

Image analysis

Laboratory troubleshooting

Education

Master's degree in biology

Tools

Zeiss Zen

FIJI-ImageJ

MS Office

TEM/SEM

Job description

Job Details:

Level: Experienced, Job Location: National Institutes of Health (NIH) - Baltimore Campus - Baltimore, MD 21224, Position Type: Full Time, Education Level: Masters Degree, Salary Range: $135,000.00 - $145,000.00 Salary/year, Travel Percentage: None, Job Shift: Day, Job Category: Public Health, Position Objective: The Biologist will support services to satisfy the overall operational objectives of the National Institute on Aging (NIA) within the National Institute of Health. The primary objective is to provide research services and deliverables through performance of support services for the Confocal Imaging Facility core of the NIA IRP.

Duties and Responsibilities:

• Study basic principles of plant and animal life, such as origin, relationship, development, anatomy, and function
• Identify, classify, and study structure, behavior, ecology, physiology, nutrition, culture, and distribution of plant and animal species
• Proficiency in Confocal Microscopy image acquisition and analysis 1
• Proficiency in Immunohistochemistry IHC and ICC and immunofluorescence IF techniques 4
• Functional knowledge of super-resolution microscopy techniques 5
• Sample preparation for transmission electron microscopy (TEM); fixation, resin embedding, ultrathin sectioning, post staining, immunogold labeling and negative staining. 2
• Preparation of solutions and chemicals 3
• Troubleshooting and optimizing laboratory protocols and instrumentation.
• User training and support.
• Operate laboratory equipment to analyze samples
• Analyze laboratory findings
• Operation of the TEM and support equipment
• Responsible for a clean and organized laboratory.
• User training and support.
• Program and use computers to store, process, and analyze data
• Interpret research or operational data
• Record research or operational data
• Pursue the research goals of the laboratory by conducting wet-lab works
• Work with staff on performing various experiments and procedures
• Harvest and collect mouse embryos, tissues and organs
• Perform histological techniques, including tissue andor whole-mount in sit hybridization and immunohistochemistry
• Prepare and organize various lab samples
• Conduct molecular biological analyses
• Provide a broad range of technical assistance to laboratory members
• Maintain laboratory supplies, equipment and experimental records
• Provide support for ordering reagents and equipment required for the laboratory
• Work products and documents related to conducting wet-lab works including harvesting and collecting mouse embryos, tissues and organs; perform histological techniques; conduct various molecular biological analyses.
• Work products and documents related to maintaining the laboratory’s supplies, equipment and experimental records.
• Clean Equipment, Run Validation, Inspect Equipment, and Meet with lab members to present updates
• Work products and documents related to training and supervising researchers in confocal microscopy

Qualifications: Basic Qualifications:

• Master’s degree in biology or a related field
• Proficiency in Confocal Light Microscopy
• Must have 4 years demonstrated experience in biological research setting with routine classic embedding and sample preparation techniques including ultramicrotomy for electron microscopy methods.
• Skilled in Zeiss Zen, FIJI-Image J, and MS Office
• Experience with cryo-fixation preparation techniques for cryo
• Demonstrated 2-5 years of experience using Scanning Electron Microscopes (SEMs) or Transmission Electron microscopes (TEMs).
• Experience with Focused Ion Beam Scanning
• Familiarity with automated imaging systems with either light or electron microscopes in a biological research laboratory,
• Experience with digital imaging of biological systems using light microscopy.
• Familiarity with workflow using 3D reconstruction software.

Preferred Qualifications:

• Electron microscopy is a plus
• Electron Microscopy or other volumetric electron microscopy techniques is a plus.
• Ability to communicate effectively, orally and in writing, with non-technical and technical staff
• Detail-oriented and possess strong organizational skills with the ability to prioritize multiple tasks and projects

*This job description is not designed to cover or contain a comprehensive listing of activities, duties or responsibilities that are required by this position.

To perform this job successfully, an individual must be able to perform each essential duty satisfactorily. The requirements listed above are representative of the knowledge, skill, and/or ability required. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.

Qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, protected veteran status or other characteristics protected by law.

This position is contingent upon contract award.