Bench Scientist 42456

Makro Scientific; Andover (MA); On-site; USD 65,000 - 85,000; Full time

Job summary

Makro Scientific is hiring a Quantitative Cellular-Biomarker Scientist in Andover, Massachusetts. This role requires skilled cell biologists/immunologists to perform cellular assays using advanced techniques like flow cytometry and help in biomarker discovery through various methodologies.

The ideal candidate should hold a BS/MS degree and possess 3-6 years of relevant experience in laboratory settings. Responsibilities include deep immune-phenotyping, receptor quantification, and operating sophisticated laboratory equipment.

Qualifications

• 3-6 years of laboratory work experience in academic or industry setting.
• Deep knowledge of human and mouse immunology.
• Proven expertise in multi-color flow cytometry.

Responsibilities

• Perform deep immune-phenotyping and phospho-signaling assays.
• Independently operate, calibrate, maintain flow cytometers.
• Assist in assay and reagent development for biomarker discovery.

Skills

Cellular and functional assays using flow cytometry

Cell culture techniques

Immune staining for multi-color flow assays

Biochemical techniques

Receptor quantification and internalization

Education

BS/MS in immunology, cell biology, or related fields

Tools

Flow cytometers

Mass cytometry (CyTOF)

Microscopes (time-lapse and confocal)

Job description

Job Description

Quantitative cellular-Biomarker scientist (Pharmacokinetics Dynamics & Metabolism) (contingent worker).

skilled cell biologist/immunologist to join a state-of-the‑art cellular biomarker‑biomeasures laboratory in PDM, Expertise in cellular and functional assays using flow cytometry, cell culture and other immunological assays is required. The lab based role entails performing deep immune‑phenotyping and phospho‑signaling assays of human, mouse and cynomolgus cells, receptor quantification, receptor internalization, and receptor occupancy to support portfolio projects. This position requires deep knowledge of human and mouse immunology, proven expertise in multi‑color flow cytometry and biochemical techniques (antibody conjugation to fluorochromes) and a strong background in quantitative cell biology. Extensive experience with cell Biological techniques such as cell culture (cell lines as well as primary immune cells), cell isolation from human blood and mouse tissues, immune‑staining for multi‑color flow assays is required, Expertise in the operation and maintenance of flow cytometers

Qualifications

BS/MS level education in immunology, cell biology or related fields required, 3-6 years of experience in laboratory work in academic or industry setting.

Additional Information

Prepare and stain cells for receptor quantification, receptor internalization kinetics, immune‑phenotyping, functional assays and microscopy. Run flow assays under minimal supervision; initiative in method development is expected. Independently carry out daily operation, calibration, maintenance and troubleshooting of flow cytometers and time‑lapse microscopes. Help in assay and reagent development, sample preparation and analysis for biomarker discovery using mass cytometry (CyTOF). Expertise in the operation and maintenance of microscopes (time‑lapse and confocal). Expertise in biochemical assays using MSD and Octet platforms. Open to learning assays for biomarker analysis using mass spectrometry.