Bacterial Immunology Research Assistant

Beth Israel Lahey Health; Boston (MA); On-site; USD 21,000 - 33,000; Full time

Job summary

Beth Israel Lahey Health is seeking an entry-level Research Assistant for the Kostic Laboratory. You will perform bench experiments spanning bacterial culture, cellular immunology, and animal studies, with accurate documentation of work.

Responsibilities include culture under anaerobic conditions, EV purification and characterization, immune cell culture, and immunoassays such as ELISA and flow cytometry, alongside in vivo mouse work and data analysis.

Qualifications

• Bachelor's degree in biology, biochemistry, immunology, microbiology, or related field.
• Entry level with hands-on laboratory research experience.
• Preferred: mouse handling, flow cytometry, cell culture, ELISA, anaerobic culture, EV characterization, chromatography, PCR/Western blot.
• Demonstrated reliability, organization, and attention to detail, with clear documentation.

Responsibilities

• Culture bacterial strains under anaerobic conditions and purify extracellular vesicles (EVs) by tangential-flow filtration and SEC.
• Characterize EVs by NTA, electron microscopy, and endotoxin testing; prepare samples for mass spectrometry.
• Culture mouse bone marrow-derived dendritic cells and human monocyte-derived dendritic cells.
• Perform cytokine ELISAs and multicolor flow cytometry to assess immune responses.
• Support in vivo mouse studies with gavage dosing and weekly glucose monitoring; maintain accurate records.
• Harvest tissues for immunophenotyping and assist with flow cytometry and histology.
• Ensure compliance with IACUC and safety regulations; work with PBMCs from healthy and Type 1 diabetes donors.
• Maintain electronic lab notebook, organize reagents, manage inventory, and maintain equipment.
• Analyze and summarize data using FlowJo and GraphPad Prism; perform routine data analyses in R or Python.
• Other duties as assigned.

Skills

Lab techniques

Flow cytometry

Cell culture

ELISA

Mouse handling

Data recording

Education

Bachelor's degree in biology/related field

Tools

FlowJo

GraphPad Prism

R

Python

Job description

Beth Israel Lahey Health is seeking an entry-level Research Assistant for the Kostic Laboratory. You will perform bench experiments spanning bacterial culture, cellular immunology, and animal studies, with accurate documentation of work.

Responsibilities include culture under anaerobic conditions, EV purification and characterization, immune cell culture, and immunoassays such as ELISA and flow cytometry, alongside in vivo mouse work and data analysis.