Associate Scientist or Scientist, DMPK and ADME

Ambrosia Biosciences Inc.; Boulder (CO); On-site; USD 80,000 - 120,000; Full time

Benefits offered by this job

Medical, dental and vision insurance

Disability and life/AD&D insurance

401(k) with 4% match

Unlimited paid time off

Job summary

Ambrosia Biosciences in Boulder, CO is hiring for an Associate Scientist or Scientist to drive in vitro ADME and bioanalytical work in a hands-on lab setting. You will quantify compounds, analyze PK data and support project teams with weekly data delivery.

Ideal candidates have strong ADME/bioanalytical experience, LC-MS/MS expertise, and the ability to coordinate CRO studies. The role offers a collaborative environment and opportunities in obesity/metabolic disease research.

Qualifications

• Hands-on in vitro ADME or bioanalytical experience in industry or academia.
• Experience with LC-MS/MS bioanalysis of in vivo PK samples.
• Ability to plan and run experiments independently and meet weekly deadlines.

Responsibilities

• Run weekly in vitro ADME screens (MDCKII, MDR1, metabolic stability).
• Develop and run LC-MS/MS methods on SCIEX instruments for plasma/tissue samples.
• Analyze PK data and perform noncompartmental analysis.
• Coordinate CRO studies and track progress.
• Maintain cell lines, instruments, reagents and lab records.

Skills

In vitro ADME

LC-MS/MS

Data analysis

Scientific communication

Independent planning

Education

B.S. in life sciences

Ph.D. in pharmaceutical sciences

Advanced degree preferred

Tools

SCIEX instruments

Phoenix WinNonline

CDD Vault

GraphPad Prism

Excel

Job description

At Ambrosia Biosciences, our mission is to develop innovative, orally delivered small-molecule therapies that address the global challenges of obesity and metabolic disorders. Founded in 2024 and headquartered in Boulder, Colorado, we leverage a world-class team of scientists to pioneer next-generation treatments targeting incretins and GPCRs. Backed by a premier syndicate of investors including Blue Owl Healthcare Opportunities, Redmile, Deep Track Capital, BVF Partners, and Boulder Ventures, we are committed to advancing safe, effective, and accessible therapeutics that transform lives worldwide.

As our lead oral small molecule GLP-1 candidate enters Phase 1 clinical trials and we accelerate the development of our broader preclinical pipeline, we seek passionate, driven individuals eager to make a meaningful impact in a dynamic, fast-paced environment. If you're ready to contribute to cutting-edge science with real-world applications and help shape the next generation of combinable metabolic therapies, we invite you to join our team.

Position Overview

Ambrosia's DMPK and ADME team is hiring an Associate Scientist or Scientist for its Boulder laboratory. The team runs in vitro ADME screens in house every week, quantifies compounds from in vivo studies by LC-MS/MS, analyzes pharmacokinetic data, and places specialized studies with CRO partners. Results go to medicinal chemistry and project teams each week and feed directly into compound design.

This is a hands-on, lab-based role. Its emphasis will follow the successful candidate's background: either in vitro ADME screening, centered on cell-based permeability and efflux assays, or bioanalysis and in vivo pharmacokinetics. The level will be set by the candidate's experience and qualifications.

Key Responsibilities

• Run Ambrosia's weekly in vitro ADME screens, including MDCKII permeability and MDR1 (P-gp) efflux assays with monolayer-integrity controls, and in vitro metabolic stability assays
• Develop and run LC-MS/MS methods on SCIEX triple quadrupole instruments, including MRM tuning for screening compounds and quantitation of compounds in plasma and tissue samples from in vivo studies
• Analyze pharmacokinetic data, including noncompartmental analysis in Phoenix WinNonlin
• Process, review and interpret data, load results into the company database, and report them to project teams and medicinal chemists on a weekly cycle
• Help coordinate studies at CRO partners, such as solubility, CYP and UGT inhibition, transporter and in vivo PK studies, including tracking progress and reviewing data
• Maintain the cell lines, instruments, reagents and laboratory records the assays depend on

Education & Experience

• Associate Scientist: B.S. with 2 or more years, or M.S. with up to 2 years, of hands‑on in vitro ADME or bioanalytical experience in industry or an academic laboratory
• Scientist: Ph.D. in pharmaceutical sciences, pharmacology, drug metabolism or a related discipline with up to 2 years of experience after the degree; M.S. with 3 or more years; or B.S. with 6 or more years of hands‑on ADME, DMPK or bioanalytical experience

Qualifications

• Hands‑on experience running in vitro ADME assays, such as MDCK or Caco-2 permeability, efflux transporter, metabolic stability, protein binding or solubility assays, or LC‑MS/MS bioanalysis of samples from in vivo PK studies
• Working knowledge of LC‑MS/MS on triple quadrupole instruments, ideally SCIEX, including method setup and troubleshooting
• Understanding of ADME principles and of how permeability, metabolism, protein binding and solubility bear on compound design
• Ability to plan and run experiments independently, keep accurate records, and meet weekly screening deadlines
• Clear written and verbal communication of data to scientists outside DMPK

Additional Skills

• Mammalian cell culture, particularly growing MDCK or Caco-2 monolayers for transport assays
• Phoenix WinNonlin for non‑compartmental analysis
• Design and analysis of in‑vivo PK studies in rodents
• Bioanalytical method validation, for example under ICH M10
• Exposure to PBPK or PK/PD modeling and human dose prediction
• Experience working with CROs
• Small‑molecule drug discovery experience in biotech or pharma, and interest in obesity or metabolic disease
• Proficiency with Excel and scientific data tools such as CDD Vault or GraphPad Prism
• Associate Scientist salary range: $80,000 to $100,000 a year
• Scientist salary range: $100,000 to $120,000 a year
• Eligible for an annual bonus based on company performance
• Medical, dental and vision insurance
• Short- and long‑term disability insurance, and life and AD&D insurance
• 401(k) plan with a 4% company match
• Unlimited paid time off for vacation and sick days, and paid company holidays
• Paid family and medical leave under Colorado FAMLI, with premiums paid by Ambrosia, and paid parental leave
• $60 monthly cell phone stipend

Ambrosia Biosciences is an equal opportunity employer, and all qualified applicants will receive consideration for employment without regard to race, color, religion, age, sex, national origin, disability status, genetics, protected veteran status, sexual orientation, gender identity or expression, or any other characteristic protected by federal, state or local laws.

Ambrosia Biosciences participates in E‑Verify to confirm the employment eligibility of all newly hired employees. Please click on the following links for more information:

Right to Work: https://www.e-verify.gov/sites/default/files/everify/posters/IER_RightToWorkPoster%20Eng_Es.pdf