Assistant Scientist – Molecular Operations (Short-Term Contract)

nuCare Medical Solutions, Inc; Frederick (MD); On-site; USD 60,000 - 80,000; Full time

Job summary

nuCare Medical Solutions, Inc seeks an Assistant Scientist for a short-term contract in an on-site laboratory. The role centers on executing bench-scale molecular biology experiments with emphasis on PCR assays, sample preparation, and routine laboratory maintenance.

The candidate will handle nucleic acid extraction, perform qPCR/RT-PCR workflows, and document data in ELN while adhering to SOPs and safety standards.

Qualifications

• BS/MS in Molecular Biology, Biochemistry, Biotechnology, or related life sciences.
• 1–3 years of hands-on lab experience running PCR/qPCR workflows.
• Experience with nucleic acid extraction and standard or quantitative PCR.

Responsibilities

• Perform routine and high-throughput PCR assays (qPCR, RT-PCR, standard end-point PCR) per SOPs.
• Extract, isolate, and quantify DNA/RNA from various matrices.
• Prepare master mixes, plates, primers/probes, and standard curves with precision to minimize cross-contamination.
• Analyze run data (Ct values, melt curves) and document results in ELN.
• Maintain inventory and calibration of thermocyclers and related equipment.

Skills

PCR workflows

qPCR

Nucleic acid handling

Aseptic technique

Pipetting accuracy

Education

BS/MS in Molecular Biology

Tools

QuantStudio

Bio-Rad CFX

Job description

Job Description: Assistant Scientist – Molecular Operations (Short-Term Contract)

Position Title: Assistant Scientist (PCR Operations)

Employment Type: Short-Term Contract

Location: On-site (Laboratory)

Position Overview

We are seeking a detail-oriented and self-motivated Assistant Scientist for a short-term contract to execute bench-scale molecular biology experiments, with a primary focus on PCR assay execution, sample preparation, and laboratory maintenance. The ideal candidate brings hands-on experience with nucleic acid extraction and standard or quantitative PCR, capable of delivering reliable data in a fast-paced laboratory environment.

Key Responsibilities

• Experimental Execution: Perform routine and high-throughput PCR assays (e.g., qPCR, RT-PCR, standard end-point PCR) following established standard operating procedures (SOPs).
• Sample Processing & Preparation: Extract, isolate, and quantify DNA/RNA from various biological matrices using manual and automated methods.
• Reagent & Assay Setup: Prepare Master Mixes, reaction plates, primers/probes, and standard curves with high precision to minimize cross-contamination.
• Data Analysis & Recording: Analyze run data (e.g., $C_t$ values, melt curves, agarose gel electrophoresis), document results promptly in Electronic Lab Notebooks (ELN), and flag anomalies to senior leadership.
• Laboratory Support & Maintenance: Maintain organized inventory of reagents and consumables, calibrate/maintain thermocyclers and liquid handling equipment, and ensure adherence to safety standards.

Qualifications & Requirements

• Education: B.S. or M.S. in Molecular Biology, Biochemistry, Biotechnology, or a related life sciences discipline.
• Experience: 1–3 years of hands-on laboratory experience running PCR/qPCR workflows (industry experience preferred).
• Core Technical Skills:
• Proficient in aseptic technique, pipetting precision, and nucleic acid handling.
• Familiarity with PCR instrument software (e.g., QuantStudio, Bio-Rad CFX) and data interpretation.
• Solid understanding of standard laboratory safety and biosafety protocols.
• Soft Skills: High attention to detail, strong organizational skills, and the ability to work independently under tight contract timelines.