Antibody Engineer II - AI-Driven Discovery & Characterization

Nautilus Biotechnology; San Carlos (CA); On-site; USD 140,000 - 210,000; Full time

Job summary

Nautilus Biotechnology in California seeks a Scientist II, Antibody Engineering & Characterization to advance antibody probes for the proteome platform. You will own antibody characterization and contribute to design rules from integrated sequence, structure, and biophysical data.

The role blends hands-on protein engineering with in silico design, driving affinity maturation strategies and ensuring data-driven improvements across the discovery pipeline. Collaboration across teams is essential.

Qualifications

• Direct experience characterizing antibodies and protein-protein interactions with BLI, SPR, Western blot, ELISA and/or other biophysical methods.
• Hands on experience developing, troubleshooting, and fine-tuning assays across multiple technologies such as SPR, BLI, ELISA, Western blot, etc.
• Experience with structure-prediction and modeling tools.
• PhD in protein engineering, structural biology, biochemistry or related fields +2-3 years of relevant industry experience.

Responsibilities

• Apply protein engineering, antibody discovery, and affinity maturation strategies to design antibody variants with improved binding affinity.
• Perform antibody purification, screening and characterization (e.g., BLI, SPR, DSF, SEC/SEC-MALS, Simple Western, ELISA) to validate outcomes.
• Identify, evaluate and implement new technologies and methods for antibody characterization and performance.
• Apply AI/ML and in silico tools to guide antibody affinity maturation and generate variant designs for iterative design-build-test-learn cycles.
• Coordinate screening of antibodies on the Nautilus platform and integrate on-platform performance with off-platform data.
• Communicate data clearly and translate results into actionable recommendations for prioritization.

Skills

Antibody engineering

Affinity maturation

AI/ML tools

Protein-protein interactions

Education

PhD in protein engineering, structural biology, biochemistry or related fields

Tools

BLI

SPR

Western blot

ELISA

Job description

Nautilus Biotechnology in California seeks a Scientist II, Antibody Engineering & Characterization to advance antibody probes for the proteome platform. You will own antibody characterization and contribute to design rules from integrated sequence, structure, and biophysical data.

The role blends hands-on protein engineering with in silico design, driving affinity maturation strategies and ensuring data-driven improvements across the discovery pipeline. Collaboration across teams is essential.