Biotechnologist (Upstream)

Tek Systems; Singapore; On-site; SGD 50,000 - 70,000; Full time

Job summary

A leading biotech company in Singapore is seeking a motivated Biotechnologist to manage upstream operations in Cell Culture. The role involves bioreactor operation and maintenance, ensuring production quality of clinical and commercial biologics while adhering to strict GMP guidelines. Candidates should possess a degree in a related life-science field with hands-on experience in cell culture or fermentation processes.

Qualifications

• Studied in Biotechnology, Biochemical Engineering, Microbiology, or related life-science field.
• Minimally internship experience in mammalian cell culture or microbial fermentation.
• Familiarity with GMP, GCP, and GDP standards.

Responsibilities

• Execute routine and critical manufacturing operations within the Cell Culture area.
• Prepare and operate bioreactors, monitor culture parameters.
• Conduct viability and sterility checks under sterile conditions.

Skills

Aseptic technique

Cleanroom behavior

Documentation skills

Education

Degree in Biotechnology

Degree in Biochemical Engineering

Degree in Microbiology

Tools

Sartorius Biostat

Ambr

Job description

• Bioreactor Operations
• Media and Reagent Preparation
• Cell Banking & Subculturing

Role Summary

We are looking for a motivated Biotechnologist, you will execute routine and critical manufacturing operations within the Cell Culture (upstream) area, ensuring consistent production of clinical and/or commercial biologics. You will operate and maintain bioreactors and related equipment in compliance with GMP guidelines and SOPs, contributing to process reliability and product quality.

Key Responsibilities

• Bioreactor Operations:
  • Prepare, set up, and operate single-use and stainless-steel bioreactors (from 50 L to 2 000 L scale).
  • Monitor and adjust culture parameters (pH, DO, temperature, agitation) to maintain optimal cell growth.
• Media and Reagent Preparation:
  • Formulate and sterilize culture media, feed solutions, and buffers.
  • Perform buffer exchanges and feed additions according to batch protocols.
• Cell Banking & Subculturing:
  • Thaw, expand, and maintain working and master cell banks under sterile conditions.
  • Conduct viability and sterility checks (trypan blue exclusion, microscopic inspection).
• Sampling & In-Process Testing:
  • Collect and process samples for cell density, viability, metabolite, and metabolomic analyses.
  • Document results accurately in electronic batch records.
• Equipment Cleaning & Sterilization:
  • Execute CIP/SIP procedures on reactors, tubing, and ancillary equipment.
  • Maintain cleanroom attire discipline and adhere to gowning/ungowning protocols.
• Documentation & Compliance:
  • Follow SOPs, batch records, and deviation/incident reporting processes.
  • Participate in periodic audits, investigations, and continuous improvement initiatives.
• Cross-Functional Support:
  • Collaborate with Purification, Quality, and Engineering teams to troubleshoot process deviations.
  • Assist in scale-up activities and technology transfer to downstream processes.

Must-Have Qualifications

• Studied in Biotechnology, Biochemical Engineering, Microbiology, or related life-science field.
• Minimally internship experience of hands-on experience in mammalian cell culture or microbial fermentation at production scale.
• Familiarity with GMP, GCP, and GDP standards in a biologics manufacturing environment.
• Strong aseptic technique and cleanroom behavior.
• Experience with single-use bioreactor systems and associated control software (e.g., Sartorius Biostat, Ambr).
• Proficient in documentation.

We regret to inform that only shortlisted candidates will be notified.

EA Registration No:R22109832,LEE YAO HONG MALCOLM

Allegis Group Singapore Pte Ltd, Company Reg No. 200909448N, EA License No. 10C4544