PhD Student Biophysics of Cellular Immunity

UMC Utrecht; Utrecht; On-site; EUR 36,000 - 45,000; Full time

Benefits offered by this job

Salary €3,217–€4,077 per month

Year-end bonus 8.3%

Holiday allowance 8%

Pension ABP

Public transport reimbursement

Reimbursement per km €0.18

Professional development opportunities

Additional benefits options

Job summary

Vorselen Lab at the Center for Translational Immunology (CTI) in UMC Utrecht is seeking a motivated, quantitative immunology researcher. You’ll develop image analysis pipelines and perform experiments combining immune assays with high-resolution fluorescence imaging to measure cellular forces during phagocytosis and cytotoxic T cell activity.

Based in Utrecht, you will work in a collaborative, bench-to-bedside environment with access to state-of-the-art facilities, including flow cytometry and

Qualifications

• Master's degree in biophysics, bionanoscience or bioinformatics (and biocomplexity), or life sciences with quantitative skills.
• Experience with programming and image analysis; eager to build new image analysis pipelines.
• Hands-on wet lab experience, including cell culture and fluorescent imaging.
• Meticulous, proactive, flexible, responsible, and able to work independently or in a team.

Responsibilities

• Develop new analysis pipelines to increase throughput of cellular force analysis during phagocytosis and cytotoxic T cell activity.
• Conduct experiments combining immune assays and high-resolution fluorescence imaging to acquire data.
• Balance experiments and analysis, with emphasis on analysis (75%).
• Contribute to interpreting biophysical parameters as potential biomarkers.

Skills

Programming

Image analysis

Quantitative skills

Teamwork

Education

Master's degree in biophysics or related

Tools

Flow cytometry

Fluorescent imaging

Job description

Mechanical forces have emerged as key regulators of immune functions. In the Vorselen Lab, we develop new technologies to tune the physical information that cells receive and to measure cellular forces. We combine this approach with high-resolution microscopy and molecular biology approaches. Together, this combination provides a very detailed readout of immune cell behavior and helps us understand the regulation of immune processes by physical forces in health and disease.

In this project, you will focus especially on developing new analysis pipelines to allow increased throughput analysis of cellular forces during phagocytosis and by therapeutic cytotoxic T cells. You will conduct experiments combining immune assays and high-resolution fluorescence imaging to obtain the required data. The balance of experiments and analysis is expected to be 25% and 75% respectively. Together, this approach is expected to reveal new insights into the regulation and functional role of biophysical forces by immune cells. The new tools and insights from this research will be a step towards assessing the potential of biophysical parameters as biomarkers.

You will work in the laboratory of Dr. Daan Vorselen which is focused on biophyiscs and quantitative innate immunity. We are hosted in the computational immunology cluster within the Center for Translational Immunology (CTI) at the UMC Utrecht. The Vorselen group (https://vorselenlab.com/) is composed of a small group with diverse academic backgrounds. The CTI is a well facilitated department with experts in all fields of immunology. We are structured to facilitate bench-to-bed side research. The CTI, or withing the UMCU/UU, we have state-of-the-art research facilities, including flow cytometry and imaging.

We’re looking for a motivated, enthusiastic candidate who is excited by quantitative approaches to cellular immunology research.

You bring:

• A master’s degree (or equivalent) in biophysics, bionanoscience or bioinformatics (and biocomplexity), or a degree in life sciences combined with clear evidence of quantitative skills.
• Experience with programming and image analysis and excitement to build new image analysis pipelines
• Hands-onwet lab experience, including cell culture and fluorescent imaging.
• A meticulous, proactive, and flexible working style, with a strong sense of responsibility.
• Excellentcommunicationandorganizationalskills,and theabilityto work independently and as part of a team.

• A salary between € 3.217,- and € 4.077,- gross per month (salary scale OIO's), based on full‑time employment (36 hours).
• Year‑end bonus of 8.3% and holiday allowance of 8%.
• Pension insurance with ABP. We take care of approximately 70% of the monthly contribution.
• 100% public transport reimbursement. Are you coming on foot, by bike or by car? You will then receive a reimbursement of € 0.18 per km (by car up to a max. of 40 km one way).
• Possibilities to develop yourself personally and professionally.
• The option to select additional employment benefits in exchange for gross salary, such as purchasing a bicycle and memberships.