Research Fellow, Molecular Pharmacology & TRP Channels

University of Southampton; Southampton; On-site; GBP 36,000 - 48,000; Full time

Benefits offered by this job

Generous holiday allowance

Flexible working

USS pension

Health and fitness facilities

Staff discounts

Job summary

The University of Southampton invites applications for a Research Fellow to join an HFSP-funded project examining how sensory receptor and ion channel systems evolved in early animals, with emphasis on TRP channels from non-bilaterian organisms.

You will apply molecular cloning, heterologous expression and pharmacological assays to characterise candidate receptors, express proteins in mammalian cells, and perform medium- to high-throughput screening to derive pharmacological profiles.

Qualifications

• PhD or near completion in pharmacology, molecular biology, neuroscience, biochemistry or evolutionary biology.
• Experience in molecular biology, cloning and construct preparation.
• Experience with heterologous expression systems and mammalian cell culture.
• Experience with functional receptor or ion-channel assays using fluorescence- or luminescence-based readouts would be desirable.
• Ability to analyse quantitative experimental data, including concentration–response curves and statistical analyses.

Responsibilities

• Characterise candidate sensory receptors and TRP channels at molecular and functional levels.
• Generate and validate receptor and channel constructs and express proteins in mammalian cells.
• Develop and optimise functional assays and perform medium- to high-throughput pharmacological screening.

Skills

Molecular biology

Cloning

Heterologous expression

Mammalian cell culture

Data analysis

EC50 determination

Electrophysiology

Python

R

Education

PhD or near completion

Tools

Mammalian cell culture

Fluorescence readouts

Luminescence readouts

Plate-reader assays

Xenopus oocyte expression

Electrophysiology

Job description

The University of Southampton invites applications for a Research Fellow to join an HFSP-funded project examining how sensory receptor and ion channel systems evolved in early animals, with emphasis on TRP channels from non-bilaterian organisms.

You will apply molecular cloning, heterologous expression and pharmacological assays to characterise candidate receptors, express proteins in mammalian cells, and perform medium- to high-throughput screening to derive pharmacological profiles.