Research Assistant (Crossley Group) (Fixed Term)

University of Cambridge; Cambridge; On-site; GBP 28,000 - 36,000; Full time

Job summary

University of Cambridge is seeking an experienced Research Assistant to join the Crossley laboratory on R-loop biology projects. You will support mammalian cell culture, molecular biology techniques and contribute to publication-ready work.

You will work with Dr Mawer to start new projects, prepare reagents, maintain stocks, and help run the day-to-day lab operations while potentially pursuing a postgraduate course if offered.

Qualifications

• Experience in mammalian cell culture and molecular biology techniques.
• Competence in cloning, qPCR, Western blotting.
• Familiarity with flow cytometry or immunoprecipitations is desirable.
• Analytical mindset and ability to work collaboratively in a group.

Responsibilities

• Contribute to ongoing projects with the aim of publication.
• Prepare reagents and maintain lab stocks; assist day-to-day lab running.
• Support genome stability, RNA biology, chromatin or cancer biology projects.

Skills

Mammalian cell culture

Molecular biology techniques

Cloning

Western blotting

qPCR

Immunoprecipitations

Microscopy

Flow cytometry

Lentiviral infection

Bioinformatics (NGS considerations)

Education

Bachelor's degree in Biology or related field

Tools

Flow cytometer

Microscope

PCR machines

Job description

We seek an experienced, scientifically curious and collaborative full-time Research Assistant to join the Crossley laboratory (https://www.cruk.cam.ac.uk/research-groups/crossley-group/) in the internationally renowned Cancer Research UK Cambridge Institute, located on the Cambridge Biomedical Campus, Cambridge, UK. You will work alongside Dr Mawer, our Senior Scientific Associate, to bring several ongoing projects to publication, as well as helping to start new projects. In the Crossley research group, we study RNA-DNA hybrid molecules called R-loops - how they are dysregulated in human disease, drive genome instability and inflammation, and how these molecules and cellular responses to them can be harnessed for cancer detection and treatment. We use biochemical, imaging and genomics approaches to study molecular mechanisms underlying R-loop biology and their clinical applications.You will have strong molecular biology skills (cloning, western blotting, lentiviral infection, qPCR, nucleic acid isolation, immunoprecipitations, microscopy, and flow cytometry) and previous experience of mammalian cell culture. In-depth expertise in at least one of genome stability, RNA biology, chromatin biology, innate immunity or cancer biology is required. Experience in next generation sequencing and/or bioinformatics will be advantageous. The successful candidate will also be required to take on other responsibilities, including preparation of reagents, maintenance of lab stocks and assisting with the day-to-day running of the laboratory.With recent long-term funding awarded, you will be joining the group at a formative time. You will be enthusiastic about being part of a growing group. This position is a staff vacancy at the University of Cambridge that would allow for the post holder to also undertake a postgraduate course at the University, should an offer of a place be made following the relevant competitive process.Fixed-term: The funds for this post are available for 1 years in the first instance.Please quote reference SW51133 on your application and in any correspondence about this vacancy.The University actively supports equality, diversity and inclusion and encourages applications from all sections of society.The University has a responsibility to ensure that all employees are eligible to live and work in the UK.Apply online