Proteomics Scientist

The John Innes Centre; Norwich; On-site; GBP 36,000 - 45,000; Full time

Job summary

The John Innes Centre, a prestigious research institute, invites applications for a full-time Proteomics Specialist. The successful candidate will support diverse proteomics projects, engaging with advanced mass spectrometry techniques while collaborating with researchers across disciplines. Join us in advancing scientific knowledge in an inclusive environment.

Benefits

Flexible working conditions

Professional development opportunities

Inclusive and supportive work environment

Qualifications

• Expertise in quantitative proteomics methods is essential.
• PhD in relevant area or equivalent experience required.
• Proficiency with mass spectrometry techniques is crucial.

Responsibilities

• Operate and maintain mass spectrometers and related systems.
• Prepare and analyze samples for proteomics research.
• Contribute to research publications and presentations.

Skills

Expertise in running and analysing proteomics experiments

Proficiency in quantitative proteomics methods

Knowledge in protein separation and enrichment sciences

Excellent communication skills

Time management and organisational skills

Education

PhD or equivalent in relevant field

MSc/BSc in biological sciences

Tools

Mass spectrometry proteomics data analysis software

Job description

Hours per week 37 Project Title The role will be based in the JIC Proteomics Platform. This position is for a Proteomics Specialist, who will help serve the growing needs of proteomics-based technology at the John Innes Centre (JIC). Months Duration Indefinite

Job Description

Main Purpose of the Job

The Proteomics Scientist will work within the Proteomics Platform at JIC to provide proteomics support to a diverse array of researchers. The role will span multiple aspects of complex proteomics experiments, including advising on experimental design, instrument maintenance and operation, method optimisation, data analysis pipelines, interactions with users/clients, training, sample preparation, data processing, presentation of data, and contributions to associated manuscripts.

Key Relationships

Internal: Line manager, proteomics platform manager and, as necessary, other researchers, research support staff and students across the Institute.
External: TSL proteomics team associated with equipment sharing; other clients and collaborators from external institutions; engineers and support specialists associated with proteomics equipment

Main Activities & Responsibilities

Percentage Operate and maintain several advanced mass spectrometers including the requisite software and nano-scale liquid chromatography systems 20 Prepare samples for proteomics analysis including protein quantification, enzymatic digestion, isobaric labelling, sample clean up 20 Run samples on a nanoLCMS system with an Orbitrap Eclipse mass spec for proteomics analysis 20 Process mass spec raw data for peptide/protein identification and quantification 10 Support proteomics projects through employing efficient and accurate data evaluation techniques, placing levels of statistical confidence on data output, and clearly reporting results 10 Interact with faculty and staff and present technical data through presentations and report writing 5 Train others on procedures, operations and new technology, or methods 5 Ensure research and record keeping is carried out in accordance with good practice, Scientific Integrity and in compliance with local policies and any legal requirements 5 Contribute methods, data and analysis to associated research publications 5 As agreed with line manager, any other duties commensurate with the nature of the role

Person Profile

Education & Qualifications

Requirement Importance PhD or other research role with significant proteomics experience Essential MSc/BSc or equivalent in biological sciences Essential

Specialist Knowledge & Skills

Requirement Importance Expertise in running and analysing proteomics experiments Essential Proficiency in quantative proteomics methods, such as isobaric labelling m Essential Proficiency with common mass spectrometry proteomics data analysis software Essential Knowledge in protein separation and enrichment sciences. Essential Knowledge and interest in biological protein structure, function and interaction. Essential Proficiency in data dependent and data independent acquisition modes Desirable

Requirement Importance Experience of oral research presentations and written presentations Essential Experience of supervising, training and mentoring visitors and students Desirable Preparing data / information for grant proposals Desirable Experience in working with different types of mass spectrometers including Orbitrap and MALDI-TOF Desirable Experience with mass spectrometry imaging Desirable

Interpersonal & Communication Skills

Requirement Importance Excellent communication skills, both written and oral, including the ability to present complex information with clarity Essential Good interpersonal skills, with the ability to work as part of a team Essential Demonstrated ability to work independently, using initiative and applying problem solving skills Essential Excellent time management and organisational skills Essential Ability to collaborate with internal and external stakeholders Essential Ability to follow instructions/Standard Operating Procedures Essential Promotes and strives for continuous improvement Essential

Additional Requirements

Requirement Importance Promotes equality and values diversity Essential Willingness to embrace the expected values and behaviours of all staff at the Institute, ensuring it is a great place to work Essential Able to present a positive image of self and the Institute, promoting both the international reputation and public engagement aims of the Institute Essential Ability to maintain confidentiality and security of information where appropriate Essential Willingness to work outside standard working hours when required Essential Ability to undertake occasional travel (national and international) related to collaborations and/or seminars Essential Attention to detail Essential

Who We Are

The John Innes Centre is an independent, international centre of excellence in plant and microbial genetics, carrying out fundamental and strategic research, to train scientists and making our findings available to society.

Our research makes use of a wide range of disciplines in the biological and chemical sciences including; cell biology, biochemistry, chemistry, genetics and molecular biology.

We pride ourselves on being a welcoming and inclusive working environment for all. We have a diverse and multicultural scientific community and thrive on our European and international links, appointing staff from across the world. Any candidate who would like further information on current or anticipated immigration requirements can contact the HR Team on [phone] or [e-mail].

We are proud to hold a prestigious Athena SWAN Gold award , recognising the impact of our work in promoting gender equality in science. We are also a proud member of the Stonewall Diversity Champion’s programme , a founding signatory of the Tecnician Commitment and, as a Disability Confident employer, guarantee to offer an interview to all disabled applicants who meet the essential criteria for the post.

The John Innes Centre is a registered charity (No. 223852), limited by guarantee (registered in England No. 511709).

Department

Biochemistry and Metabolism

Researchers within the Department of Biochemistry and Metabolism investigate the structure and function of biological macromolecules and fundamental aspects of plant and microbial metabolism. They aim to understand the mechanisms underpinning metabolic diversity and how to manipulate proteins, carbohydrates, small molecules and DNA, research which is facilitating advances of agricultural, environmental, medical and industrial importance.

The position will be placed in the JIC Proteomics Facility team and help provide a comprehensive Proteomics service to JIC, as well as for the wider Norwich Research Park and other external partners.

Proteomics Scientist
Salary: £36,720 - £44,500 per annum depending on qualifications and experience.
Contract: Indefinite, full-time.
Location: John Innes Centre, Norwich, UK.
Closing date: 23 July 2025
Reference: 1004842

The John Innes Centre (JIC) seeks a talented, motivated individual as a proteomics specialist to serve the growing needs of proteomics-based technology at JIC, which is a globally recognized, independent research institute specializing in plant and microbial sciences.

About the Proteomics Platform Group:

The position will be in the JIC Proteomics Platform team, which provides a comprehensive Proteomics service to JIC, as well as for the wider Norwich Research Park and other external partners. The platform is equipped with state-of-the-art mass spectrometers, including a Thermo Orbitrap Eclipse Tribrid equipped with aFAIMS PRO DUOinterface. A MALDI source is also available to perform MALDI imaging on the Orbitrap Eclipse.

Further instruments include a Bruker MALDI-TOF and a Waters Synapt G2-Si Q-TOF. These facilities allow for a broad range of methods including analysis of individual proteins, the qualitative and quantitative analysis of complex protein samples (e.g. CoIPs, expression studies, modifications, isotope labelling), as well as mass spectrometry imaging and ion mobility. We offer collaboration, service and advice across the Norwich Research Park, the wider research community and commercial users.

The role:

Applications are invited for an enthusiastic and talented proteomics specialist to join our Proteomics Platform in a full-time, indefinite post. The specialist will work alongside an experienced platform manager to support proteomics projects, including instrument maintenance and operation, sample preparation, method optimization, data analysis pipelines, interactions with users/clients, advising on experimental design, and training. The specialist will also assist with data processing and contribute to associated research papers.

While the work is focused on proteomics on the Orbitrap Eclipse, the role will also involve the operation and maintenance of several advanced mass spectrometers (Thermo Orbitrap Eclipse, Bruker MALDI-TOF, Waters Synapt Q-TOF) including the requisite software and nano-scale liquid chromatography systems. The specialist will prepare samples for proteomics analysis including protein quantification, enzymatic digestion, isobaric labelling, and sample clean up. Projects will be further supported by employing efficient and accurate data evaluation techniques, and the role will involve close interactions with research groups to aid with experimental design, data analysis and associated training.

The ideal candidate:

The ideal candidate will have substantial experience in proteomics-based work and a PhD in a relevant subject, or equivalent research experience in proteomics. Hands-on experience in the application of mass spectrometry/LC-MS techniques to the detailed characterization of proteins is essential for this role. Prior experience of running quantitative MS experiments is essential.

The candidate will have knowledge in protein separation and enrichment sciences, as well as an interest in biological protein structure, function and interactions. Knowledge and proficiency in the operation of advanced Orbitrap, MALDI and Q-TOF mass spectrometers is highly desirable, along with associated liquid chromatography systems.

The candidate should be skilled in the preparation of high-quality samples for advanced experiments, such as protein quantification and isobaric labelling. Proficiency with common mass spectrometry proteomics data analysis software is desirable (e.g. Mascot, Proteome Discoverer, MaxQuant, MSFragger, DIA-NN, Skyline), along with experience in handling large data sets and performing statistical data analysis of proteomics results. Experience in working with different types of mass spectrometers, such as mass spec imaging (MALDI-imaging), is desirable.

While experience in instrument maintenance is highly desirable, the platform will provide full training across all relevant instruments and techniques, ensuring you’ll be equipped to handle a variety of tasks.

Additional information:

This role meets the criteria for a visa application, and we encourage all qualified candidates to apply. Please contact the Human Resources Team if you have any questions regarding your application or visa options.

We are an equal opportunities employer, actively supporting inclusivity and diversity. As a Disability Confident organisation, we guarantee to offer an interview to all disabled applicants who meet the essential criteria for this vacancy. We are proud to hold a prestigious Gold Athena SWAN award in recognition of our inclusive culture, commitment and good practices towards advancing of gender equality. We offer an exciting, stimulating, diverse research environment and actively promote a family friendly workplace. The Institute is also a member of Stonewall’s Diversity Champions programme.

The John Innes Centre is a registered charity (No. 223852) grant-aided by the Biotechnology and Biological Sciences Research Council.