Protein Scientist

JR United Kingdom; London; On-site; GBP 100,000 - 125,000; Full time

Job summary

An innovative biotechnology company is on the lookout for a passionate Protein Scientist to join their team in London. This role involves designing DNA constructs to overexpress proteins, performing tissue culture, and supporting drug screening efforts. You will play a crucial role in advancing research and developing therapies that improve patients' lives. The ideal candidate will have a PhD in Biochemistry and experience in producing recombinant proteins and biophysical characterization techniques. If you are motivated and ready to make a significant impact in the biotech field, this opportunity is perfect for you.

Qualifications

• PhD in Biochemistry with 2-3 years post-doc experience in a lab or industry.
• Experience in producing recombinant proteins and biophysical characterization.

Responsibilities

• Design DNA constructs to overexpress proteins in recombinant systems.
• Perform routine tissue culture and monitor cell viability.
• Execute experiments for purification of recombinant proteins.

Skills

Protein Design

Tissue Culture

Cell Viability Monitoring

Protein Purification

Biophysical Characterization

Drug Screening

Education

PhD in Biochemistry

Tools

SDS-PAGE

Western Blots

HPLC

ELISA

Biolayer Interferometry (BLI)

Surface Plasmon Resonance (SPR)

Job description

Our client is a pioneering biotechnology company dedicated to advancing research and developing various solid tumour programs. We are committed to improving the lives of patients worldwide by harnessing the power of science and medicine. As we continue to expand our portfolio of ground-breaking therapies, we are seeking a highly motivated Protein Scientist.

Key Responsibilities:

• Design DNA constructs and/or vectors to overexpress proteins within recombinant systems.
• Perform routine tissue culture of suspension/transient mammalian cell lines under sterile conditions, monitoring aspects such as cell counts and viability. Perform cell banking, as required.
• Harvest and clarify cell culture fluid, as suitable for downstream protein purification.
• Support other drug screening efforts using cell-based assays and monitoring functional readouts, such as cell viability, proliferation and cytotoxicity.
• Design, execute and/or oversee experiments for the purification of recombinant proteins and antibodies.
• Perform experiments and/or interpret data derived from QC analyses such as SDS-PAGE, Western blots, binding ELISAs, endotoxin-testing and HPLC, biophysical analyses of proteins using techniques such as fluorescence, biolayer interferometry (BLI) and surface plasmon resonance (SPR).

Qualifications:

• PhD in Biochemistry or a related field with 2-3 years post-doc experience preferably working in a laboratory or industrial setting (Biotech/Pharma/Academia).
• Experience of producing recombinant proteins from mammalian cell lines.
• Experience of biophysical characterisation techniques, QC and/or profiling of proteins.
• Experience of screening approaches for drug discovery using ELISA and/or cell-based assays.
• This role will require on-site presence 5 days per week, thus the candidate must be already living in London or willing to relocate to London.