Postdoctoral Position

Instruct-ERIC; Greater London; Hybrid; GBP 28,000 - 35,000; Full time

Job summary

The Institute of Cancer Research in central London invites applications for a Postdoctoral position in Alessandro Vannini's lab to structurally and functionally characterize large multi-subunit complexes involved in RNA Polymerase III transcription and cancer.

Applicants should hold a PhD in biochemistry or molecular biology with experience in multi-subunit production and purification; crystallography or cryo-EM experience is desirable.

Qualifications

• PhD (or equivalent) in biochemistry or molecular biology.
• Experience in purification of recombinant and/or endogenous proteins for structural biology.
• Background in production of multi-subunit complexes; crystallography or cryo-EM desirable.

Skills

Multi-subunit complex production

Purification of proteins

Protein crystallography

Single particle electron microscopy

Education

PhD in biochemistry or molecular biology

Tools

AKTA Pure

Bruker Microstar

FEI Tecnai F20

FEI Tecnai T12

crystallisation robots

ITC

multi-angle light scattering

Job description

Jobs in Structural Biology and Related Fields

Postdoctoral Position

London, United Kingdom

A PostDoctoral position is immediately available in Alessandro Vannini's lab at the Institute of Cancer Research in central London to structurally and functionally characterise large multi-subunit complexes involved in eukaryotic transcription (www.icr.ac.uk/alessandrovannini).

We are looking for highly motivated and creative individuals with a strong interest in structural characterization of multi-subunit complexes. We currently employ single particle electron microscopy analysis and x-ray crystallography, as well as biochemical and biochemical analysis, to elucidate the role of the RNA Polymerase III transcription apparatus in cancer and other fundamental cellular processes (www.icr.ac.uk/alessandrovannini).

The lab is well equipped with a fleet of AKTAs (including a brand new AKTA Pure) and have access to divisional managed facilities for protein crystallography (Bruker Microstar and CCD detector and crystallisation robots), cryo-electron microscopy (FEI Tecnai F20 and T12), and protein production with expertise in multi-subunit expression (insect cell, yeast and bacterial expression, including a 60 L fermentor). The Division is also well equipped with equipment for biophysical analysis (e.g. ITC, fluorescence, multi-angle light scattering).

Applicants should possess a PhD (or equivalent) in biochemistry or molecular biology with a sound knowledge of multi-subunit complex production and purification. Experience in purification of recombinant and/or endogenous proteins for structural biology analysis is essential for this post. Experience in protein crystallography and/or single particle electron microscopy would be desirable. Individuals with a strong background in biochemical characterization of transcription machineries wishing to learn structural biology techniques will be taken into serious consideration.

The lab is located in the heart of central London, in the vibrant area of South Kensington.

Appointment will be on Fixed Term Contract for 2 years in the first instance, with a starting salary in the range of £28,087 to £34,529 p.a. inclusive (based on previous post-doctoral experience).

Job Ref. No. 1355633

Closing Date: 15/09/2013