Associate Scientist/Technician - CNS Biology

Hlx Life Sciences; Greater London; In loco; GBP 32.000 - 52.000; Tempo pieno

Descrizione del lavoro

Hlx Life Sciences is seeking an Associate Scientist/Technician to support CNS biology research using human iPSC-derived neuronal models. This laboratory-based role focuses on in vitro studies to advance discovery and development of novel therapeutics.

The successful candidate will establish and maintain iPSC-derived neuronal cultures, differentiate cells, and perform routine culture and quality control. Strong experience with immunocytochemistry, ELISA, Western blot and qPCR is essential.

Competenze

• Hands-on experience culturing and maintaining human iPSC-derived neurons.
• Experience differentiating hiPSCs into neuronal cell types and supporting maturation.
• Experience with immunocytochemistry, ELISA, Western blot, RNA/DNA extraction, qPCR/RT-qPCR.
• Ability to work collaboratively within a drug-discovery team.
• Strong analytical, problem-solving and organisational skills; health & safety awareness.

Mansioni

• Establish, maintain and differentiate human iPSC-derived neuronal cultures.
• Perform routine culture of iPSC-derived neurons including seeding, media prep, differentiation, maturation, passaging, cryopreservation, thawing and QC.
• Support pharmacological studies including compound preparation, dose‑response and analysis using biochemical and cellular assays.
• Develop and optimize neuronal models and assays for CNS drug discovery.
• Maintain lab organisation, reagent preparation, equipment maintenance and inventory control.

Conoscenze

iPSC-derived neurons culture

Neuronal differentiation

Immunocytochemistry

Western blotting

ELISA

qPCR/RT-qPCR

RNA/DNA extraction

Data analysis

Collaborative teamwork

Lab safety

Formazione

BSc or MSc in biology/related field

Strumenti

ELISA equipment

Western blot apparatus

Cryopreservation equipment

Descrizione del lavoro

We are seeking a highly motivated Associate Scientist/Technician to join our CNS Biology team. This laboratory-based role will focus on conducting in vitro studies using human iPSC-derived neuronal models to support the discovery and development of novel therapeutics.

The role will report to the Associate Director, Biology and involve:

• Establish, maintain and differentiate human iPSC-derived neuronal cultures, supporting their maturation and ongoing maintenance for compound screening and mechanistic studies.
• Perform routine hands‑on culture of iPSC-derived neurons, including cell seeding, media preparation, differentiation, maturation, passaging where appropriate, cryopreservation, thawing and quality control.
• Support pharmacological studies, including compound preparation, dose‑response experiments and analysis of treatment effects using biochemical and cellular assays including ELISA, Western blotting and immunocytochemistry.
• Support the development and optimisation of robust neuronal cellular models and assays for CNS drug discovery.
• Maintain high standards of laboratory organisation, reagent preparation, equipment maintenance and inventory management.

This is primarily a lab-based role (>75%), with the expectation of being onsite in Hammersmith for a minimum of four days per week.

Qualifications & experience

• BSc or MSc in a relevant biological discipline, such as neuroscience, biochemistry, pharmacology, genetics or a related field.
• Substantial hands‑on experience culturing and maintaining human iPSC-derived neurons is essential.
• Hands‑on experience differentiating human iPSCs into neuronal cell types and supporting neuronal maturation is strongly preferred.
• Candidates should have experience working routinely with iPSC-derived neuronal cultures, rather than solely non‑neuronal iPSC-derived cell types.
• Experience in molecular and cellular laboratory techniques including immunofluorescence/immunocytochemistry, Western blotting, ELISA and other protein‑based assays, RNA/DNA extraction, qPCR/RT‑qPCR and cell viability assays.
• Ability to work collaboratively within multidisciplinary drug‑discovery teams to deliver key data packages.
• Excellent analytical and problem‑solving abilities, with strong interpersonal and organisational skills.
• Comfortable working in an early‑stage environment and dealing with ambiguity and uncertainty.
• Experience in laboratory health and safety management would be desirable.