Molecular Biology & Vectorology Associate Scientist/Scientist

Alaya.bio; Villejuif; Sur place; EUR 45 000 - 60 000; Plein temps

Résumé du poste

A biopharmaceutical company in Île-de-France is seeking a talented Molecular Biology & Vectorology Associate Scientist/Scientist. The role involves designing and engineering expression vector systems, optimizing bioproduction of lentivectors, and ensuring compliance with regulatory standards. The ideal candidate should hold an MSc in Molecular Biology or equivalent, possess expertise in plasmid engineering, and have hands-on molecular biology experience. Proficiency in English is essential. This position is based in the Paris area.

Qualifications

• Proven track-record in plasmid engineering and regulatory elements.
• Experience in bioproduction of cell-based products.
• Proficiency in English is essential.

Responsabilités

• Design and optimize molecular constructs for bioproduction.
• Implement selected solutions in biomanufacturing.
• Conduct various biochemical and cellular assays.

Connaissances

Engineering of plasmid

Bioproduction in eukaryotic systems

Hands-on molecular biology

Flow cytometry

Data analysis tools (GraphPad Prism)

Formation

MSc in Molecular Biology or equivalent

PhD in relevant field

Outils

DNA design software (SnapGene, Benchling)

Description du poste

Alaya.bio is a preclinical-stage biotech company pioneering a new generation of polymeric nanoparticles applied to the rapid and universal in situ manufacturing of CAR T-cells.

Founded in July 2022 and based in Paris and Cambridge, Alaya.bio is backed by industry leaders and investors with long track-records in cell therapy. The potential of our universal in situCAR T technology has been recognized by the closing of five partnerships with renowned players in the clinical development of these groundbreaking therapies.

As part of our continuous growth, we are looking for a highly enthusiastic and talented Molecular Biology & Vectorology Associate Scientist/Scientist who will evolve at the interface between our bioproduction and R&D teams. This role will focus on the design and engineering of expression vector systems that will optimize the manufacturability, safety and efficacy of the lentivectors used as payloads for our lead nanoparticles.

The position is located in Paris area.

Key Responsabilities

• Design, plan and execute the screening and optimization of molecular constructs applied to the bioproduction of new generation lentivectors in close collaboration with our Bioprocess Development Specialist and R&D Director.
• Conduct the implementation of selected molecular solutions within internal biomanufacturing development activities (from USP to fill and finish) and the associated analytical developments.
• Management of partners and sub-contractors with whom the company has chosen to work for plasmid design and manufacturing.
• Perform a variety of biochemical (ELISA, MACSPlex™) molecular (RT-qPCR, qPCR) & cellular assays (transfection, transduction and flow cytometry).
• Cell lines culture (adherent and in suspension) for transient and stable transgenes expression studies.
• Design, develop and optimize upstream process unit operations compatible with the industrialization of the bioprocess in agreement with quality by design principles.
• Contribute to the development of appropriate quality controls alongside the optimization of the bioprocess.
• When needed, manufacture engineered lentiviral vector batches used for in vitro and in vivo functional studies.
• Collect, analyze and report data using appropriate software including electronic lab notebooks.
• Support day-to-day laboratory operations, including reagents and consumables purchasing, maintenance of equipment, management of biological, infectious & care activity wastes,…
• Compliance with regulatory standards and health and safety rules in place in the company.
• Writing of technical documents including SOPs, experimental protocols and study reports.
• Participation in the dissemination of the company's scientific results (scientific articles, communications at international conferences).

Your Profile

• MSc in Molecular Biology, Vectorology or equivalent; PhD is a plus.
• Proven track-record in the engineering of plasmid (backbone and promoter optimization, codon usage, multi-cistronic GOIs, logic gating, etc…) and regulatory elements (lcRNA, shRNA, siRNA, microRNA). Expertise in viral vector plasmid design is a plus.
• Strong expertise in the bioproduction of cell-based products in eukaryotic systems (deepwell plates, shaking flasks, bioreactors) at small (3-30 mL) and medium scale (1 to 3 L). Expertise in viral vector production is a plus.
• Hands-on expertise in molecular biology, cell culture, qPCR, RT-qPCR, bacteriology. Flow cytometry is a plus.
• Additional knowledge in the improvement of GMP-compatible bioprocess units is a plus.
• Ability to work in a fast-paced environment, meet deadlines, and prioritize work on multiple projects.
• Proficiency in English (written and spoken) is essential, given the international exposure of the company.
• Good command of IT including DNA design and sequence analysis software (SnapGene, CLC, Benchling,…) and data analysis tools (GraphPad Prism).