Engineer in Homologous Recombination (F/M)

PSL Université Paris; Paris; Sur place; EUR 40 000 - 60 000; Plein temps

Résumé du poste

A leading research institution in Paris seeks a candidate for a fixed-term position in the Compartmentalization and Dynamics of Nuclear Functions team. The successful applicant will join a project aimed at identifying inhibitors of homologous recombination through high-content screening. Candidates should hold a Master's degree in Molecular Biology or Engineering and be fluent in English. The role includes responsibilities such as cell culture experiments and molecular biology techniques. The position offers a full-time contract with various benefits.

Prestations

Collective catering

Reimbursement of transportation fees up to 70%

Supplementary health insurance

Qualifications

• Fluency in English is required, as English is the working language of the laboratory.
• Background in cell culture, molecular biology, and/or image analysis is advantageous.

Responsabilités

• Performing cell culture experiments using human cell lines, including inhibitor treatments and transfections.
• Conducting molecular biology experiments, including cloning.
• Developing and optimizing the screening pipeline in collaboration with the BioPhenics platform.
• Validating candidate targets using cellular assays.

Connaissances

Cell culture

Molecular biology

Image analysis

Interdisciplinary techniques

Formation

Master’s degree in Molecular and Cellular Biology or Engineering degree

Description du poste

Institut Curie Research Center is a major player in the research and fight against cancer. It consists of a Hospital group and a Research Center of more than 1000 employees with a strong international representativeness. The objective of the Research Center is to develop basic research and to use the knowledge produced to improve the diagnosis, prognosis, and therapeutics of cancers as part of the continuum between basic research and innovation serving the patient.

Missions

The laboratory

The successful candidate will join the Compartmentalization and Dynamics of Nuclear Functions team led by Angela Taddei at Institut Curie (https://curie.fr/equipe/taddei). Our research focuses on understanding how nuclear organization regulates genome functions, with a particular emphasis on homologous recombination. We combine state-of-the-art in vivo quantitative microscopy approaches, including super-resolution imaging, with genetic and molecular biology techniques.

The project

This position is part of a newly funded technology transfer project aimed at identifying novel inhibitors of homologous recombination. The candidate will be actively involved in all phases of the project, from the establishment of the laboratory pilot assay to the execution of a large-scale screen at the BioPhenics high-content imaging platform, followed by validation of candidate hits. This project offers a unique opportunity to work at the interface of fundamental research, advanced imaging, and translational science.

Education and competences

• Master’s degree in Molecular and Cellular Biology or Engineering degree
• Fluency in English is required, as English is the working language of the laboratory

The candidate is expected to contribute actively to the development, execution, and analysis of a high-content screening strategy. Responsibilities will include:

• Performing cell culture experiments using human cell lines, including inhibitor treatments and transfections with siRNA and plasmids
• Conducting molecular biology experiments, including cloning
• Developing and optimizing the screening pipeline in close collaboration with the BioPhenics platform, encompassing both technical implementation and data analysis
• Validating candidate targets using cellular assays such as homologous recombination assays, focus formation assays, and cytotoxicity assays

Skills and profile

• Background or hands-on experience in cell culture, molecular biology, and/or image analysis is advantageous but not mandatory
• Strong motivation to learn interdisciplinary techniques and work in a collaborative environment

Contract information

Type of contract: Fixed-term contract.

Duration: 12 months

Working time: full time

Remuneration: according to the current grids

Benefits

• Collective catering
• Reimbursement of transportation fees up to 70%
• Supplementary health insurance

Location of the position: Paris

Contact

Please send your CV and letter of motivation to

Institut Curie is an inclusive, equal opportunity employer and is dedicated to the highest standards of research integrity