PhD position: Mitochondrial Lipid Metabolism & FAO Research

Autonomous University of Madrid (Universidad Autónoma de Madrid); Spain; On-site; EUR 32,000 - 45,000; Full time

Job summary

Autonomous University of Madrid (Universidad Autónoma de Madrid) invites applications for a First Stage Researcher position in the Translational Energy Metabolism Group. The role focuses on mitochondrial fatty acid β-oxidation across life stages, employing in vitro and in vivo techniques.

You will work on transgenic mouse models, primary cell/organoid cultures, and multi-omics integration, with CRISPR/Cas9 and bioinformatics as key components of the project.

Qualifications

• Academic performance assessed from undergraduate and master's grades.
• Experience and certification for handling laboratory animals in Spain or the EU.
• Experience with methods to assess mitochondrial function and metabolism.
• Experience and autonomy in CRISPR/Cas9 technology.
• Experience and autonomy in bioinformatics.
• Experience with skeletal muscle and liver analysis using microscopy, flow cytometry and histology.

Responsibilities

• Manage transgenic mouse colonies and develop disease-relevant FAO dysfunction models.
• Culture primary cells and organoids from muscle and liver.
• Analyze mitochondrial function using oxygen consumption assays and BN-PAGE.
• Integrate multi-omics data from preclinical and clinical cohorts.

Skills

Bioinformatics

CRISPR/Cas9

Skeletal muscle analysis

Liver analysis

Education

Undergraduate degree

Master's degree

Tools

Seahorse XF24/XF96

BN-PAGE

Clark-type oxygen electrode

Flow cytometry

Microscopy

Histology

Job description

Autonomous University of Madrid (Universidad Autónoma de Madrid) invites applications for a First Stage Researcher position in the Translational Energy Metabolism Group. The role focuses on mitochondrial fatty acid β-oxidation across life stages, employing in vitro and in vivo techniques.

You will work on transgenic mouse models, primary cell/organoid cultures, and multi-omics integration, with CRISPR/Cas9 and bioinformatics as key components of the project.