PhD position (m/f/d) on immune reset through CAR T in chronic inflammatory diseases

Universitätsklinikum Erlangen; Erlangen; Hybrid; EUR 22.000 - 32.000; Vollzeit

Zusammenfassung

Universitätsklinikum Erlangen is seeking a PhD student to explore how human immune-cell networks shape tissue inflammation, regeneration and responses to cellular therapies. The project combines patient-derived material with experimental models to address mechanistic questions in cellular immunology.

You will characterize immune-cell populations, study interactions and analyze molecular data using RNA sequencing and other transcriptomic technologies, contributing to advances in immunology

Qualifikationen

• Experience with multiparameter flow cytometry is welcome.
• Interest in immune cell interactions and human disease.
• Ability to work independently and learn new techniques.

Aufgaben

• Characterizing human immune-cell populations and their functional states using multiparameter flow cytometry and high-dimensional immune phenotyping.
• Investigating cellular interactions and immune networks by integrating phenotypic, functional and molecular data from immune cells and tissues.
• Studying immune responses to cellular therapies, including the mechanisms underlying immune depletion, reconstitution and tissue regeneration after CD19 CAR-T-cell therapy.
• Generating and analyzing molecular data using approaches such as bulk and single-cell RNA sequencing and other transcriptomic or spatial technologies.
• Establishing and applying experimental models to investigate mechanisms identified in human samples, including selected mouse models of intestinal inflammation, immune reconstitution and tissue regeneration after CD19 CAR-T-cell therapy.
• Developing and combining experimental and analytical approaches ranging from established methods such as ELISA, Western blotting and flow cytometry to advanced multidimensional cytometry, UMAP-based analysis and transcriptomic approaches.
• The project offers substantial scope to develop an individual scientific profile. Previous experience with these techniques is welcome, but curiosity, scientific independence, willingness to learn, and enthusiasm for understanding how immune cells interact in human disease are similarly important.

Kenntnisse

Flow cytometry

Single-cell RNA sequencing

Bulk RNA sequencing

Transcriptomics

Immunology

Tools

ELISA

Western blotting

Flow cytometry

Jobbeschreibung

State-of-the-art medicine and nursing care you can rely on! - Since being founded in 1815, Universitätsklinikum Erlangen has offered medicine of the highest standard and incorporated the latest insights from medical research and state-of-the-art equipment into diagnosis and therapy. More than 10.000 employees from approximately 50 different occupations are needed to provide the wide range of services offered by Universitätsklinikum Erlangen, ensuring our patients receive the very best care around the clock.

As a PhD student, you will investigate how human immune-cell networks shape tissue inflammation, regeneration and response to cellular therapies. The project will focus primarily on the human immune system and combine patient-derived material with experimental models to address mechanistic questions in cellular immunology.

• Characterizing human immune-cell populations and their functional states using multiparameter flow cytometry and high-dimensional immune phenotyping.
• Investigating cellular interactions and immune networks by integrating phenotypic, functional and molecular data from immune cells and tissues.
• Studying immune responses to cellular therapies, including the mechanisms underlying immune depletion, reconstitution and tissue regeneration following CD19 CAR-T-cell therapy.
• Generating and analyzing molecular data using approaches such as bulk and single-cell RNA sequencing and other transcriptomic or spatial technologies.
• Establishing and applying experimental models to investigate mechanisms identified in human samples, including selected mouse models of intestinal inflammation, immune reconstitution and tissue regeneration after CD19 CAR-T-cell therapy.
• Developing and combining experimental and analytical approaches ranging from established methods such as ELISA, Western blotting and flow cytometry to advanced multidimensional cytometry, UMAP-based analysis and transcriptomic approaches.
• The project offers substantial scope to develop an individual scientific profile. Previous experience with these techniques is welcome, but curiosity, scientific independence, willingness to learn, and enthusiasm for understanding how immune cells interact in human disease are similarly important.