Doctoral Student (PhD / Dr. rer. nat., m/f/div., E13 TV-L 65%) (m/w/div.)

Deutsches Diabetes-Zentrum; Düsseldorf; Vor Ort; EUR 42.000 - 54.000; Vollzeit

Zusammenfassung

Das Deutsche Diabetes Zentrum (DDZ) in Düsseldorf sucht eine/n Doktoranden/in (PhD / Dr. rer. nat.) in Biochemie und Stoffwechselforschung.

Sie arbeiten an proteomischen Analysen menschlicher Skelettmuskulatur und mechanistischen Studien in Mausmodellen, um Muskelmetabolismus und Insulinwirkung besser zu verstehen. Sie verfügen über einen Masterabschluss in Biochemie/Molekulare Biologie oder einem verwandten Fach, Erfahrung in Western Blot, qPCR und Zellkultur und arbeiten selbstständig sowie im

Qualifikationen

• Master-Abschluss in einem der genannten Fächer.
• Praktische Erfahrung in molekularen und zellulären Techniken wie Western Blot, qPCR und Zellkultur.

Aufgaben

• Proteomische Analysen von menschlichen Skelettmuskelbiopsien aus Kältezulassungsstudien zur Identifikation molekularer Signaturen.
• Mechanistische Studien in wildtyp- und UCP1-defizienten Mäusen zu Wirkung von Kälteexposition und Muskeldaktivierung auf Glukoseaufnahme und Insulinwirkung.
• Funktionelle Charakterisierung etablierter und neuer Regulatoren des Skelettmuskelstoffwechsels.

Kenntnisse

Molekulare Biologie

Ausbildung

Master-Abschluss in Biochemie, Molekularbiologie, Molekulare Medizin, Physiologie, Biomedizin oder verwandtem Fach

Jobbeschreibung

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Institut für Klinische Biochemie und Pathobiochemie (Direktor: Univ.-Prof. Dr. rer. nat. Hadi Al-Hasani), Arbeitsgruppe Pathobiochemie (Leiter: Univ.-Prof. Dr. rer. nat. Hadi Al-Hasani)

The German Diabetes Center (DDZ), Leibniz Institute for Diabetes Research at Heinrich Heine University (HHU) Düsseldorf is an interdisciplinary research institute that integrates basic, translational and clinical research to improve the prevention, diagnosis and treatment of diabetes mellitus and its complications. The Institute for Clinical Biochemistry and Pathobiochemistry (Director: Prof. Dr. Hadi Al-Hasani) investigates the molecular mechanisms underlying insulin resistance, skeletal muscle metabolism and type 2 diabetes.

For a new research project, we are looking for a talented and highly motivated

Doctoral Student (PhD / Dr. rer. nat., m/f/div., E13 TV-L 65%)

Ihr Aufgabengebiet umfasst

Your Project Will Include

• Proteomic analysis of human skeletal muscle biopsies obtained from cold acclimation studies to identify molecular signatures associated with improved insulin sensitivity.
• Mechanistic studies in wild-type and UCP1-deficient mice to investigate the effects of cold exposure and muscle activation on glucose uptake and insulin action.
• Functional characterization of established and novel regulators of skeletal muscle metabolism, including AMPK, GLUT4, Rac1 and newly identified candidate proteins.
• Investigation of GLUT4 trafficking and insulin signaling using advanced molecular and cellular approaches.

The Project Offers Training In

• Mass spectrometry-based proteomics
• Mouse models of insulin resistance and metabolic disease
• Indirect calorimetry and metabolic phenotyping
• Glucose uptake assays in isolated skeletal muscle
• Molecular biology techniques (Western blotting, qPCR, gene expression analyses)
• Immunohistochemistry and skeletal muscle fibre typing
• In vivo electroporation and functional gene manipulation
• Fluorescence-based analysis of GLUT4 translocation

Wir erwarten von Ihnen

Your profile

• You hold (or are close to obtaining) a Master’s degree in Biochemistry, Molecular Biology, Molecular Medicine, Physiology, Biomedical Sciences or a related discipline.
• You have practical experience in molecular and cellular biology techniques such as Western blotting, qPCR and cell culture.
• Experience with metabolic phenotyping, skeletal muscle biology, mouse models or proteomics is advantageous but not required.
• You enjoy interdisciplinary research and are interested in combining human physiology with mechanistic laboratory studies.
• You are highly motivated, curious, and able to work independently as well as collaboratively in an international research environment.
• You have an excellent command of written and spoken English.

Wir bieten Ihnen

We provide an exciting, dynamic and international research environment with access to unique resources in diabetes and metabolism research. The successful candidate will receive comprehensive training in modern metabolic phenotyping, molecular biology and translational research, embedded in a highly collaborative environment at the German Diabetes Center and Heinrich Heine University Düsseldorf. Opportunities for scientific exchange, international collaborations and participation in advanced training programs are available through the German Center for Diabetes Research (DZD) and the institute’s graduate education activities. Initially, the position is available for two years starting from 01.01.2027 or later with the possibility of extension. The employment contract is governed by the current version of the TV-L regulations for the State of North Rhine-Westphalia, including the provisions applicable to scientific staff. Remuneration will be according to TV-L E13 (65%), depending on qualifications and experience.

Female candidates are particularly encouraged to apply.

Applications from persons with disabilities will be given preferential consideration where qualifications are equivalent.

The German Diabetes Center is committed to equal opportunities, diversity and family-friendly working conditions and has been certified under the "Career and Family" audit since 2011.

The provisions of

• 7 (1) of the German Part-Time and Fixed-Term Employment Act (TzBfG) apply.