Postdoctoral position in multiplexed single-cell RNAseq-based antibody discovery

École polytechnique fédérale de Lausanne, EPFL; Lausanne; Vor Ort; CHF 85.000 - 110.000; Vollzeit

Zusammenfassung

EPFL in Lausanne invites applications for a Postdoctoral position in multiplexed single-cell RNAseq-based antibody discovery. The LBMM lab seeks candidates with a strong background in small-scale transcriptomics and antibody discovery, ideally familiar with 10X Genomics or DropSeq and flow cytometry.

Interdisciplinary collaboration with bioinformatics and microfluidics experts is expected. The successful candidate will optimize sequencing workflows, prepare libraries, analyze data, and help

Qualifikationen

• Hands-on experience in single-cell analysis and/or antibody discovery.
• Knowledge of single-cell RNAseq workflows such as 10X Genomics or DropSeq, experienced in flow cytometry.
• Knowledge in cell biology and biochemistry.
• Prior experience in microfluidics is advantageous but not required.

Aufgaben

• Optimization of single-cell sequencing workflows
• FACS sorting of cells
• Preparation of NGS libraries
• Data analysis
• Experimental design of the screens together with bioinformaticians

Kenntnisse

Single-cell analysis

Antibody discovery

RNAseq workflows

Tools

10X Genomics platform

DropSeq

Flow cytometry

Jobbeschreibung

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EPFL, the Swiss Federal Institute of Technology in Lausanne, is one of the most dynamic university campuses in Europe and ranks among the top 20 universities worldwide. The EPFL employs more than 6,500 people supporting the three main missions of the institutions: education, research and innovation. The EPFL campus offers an exceptional working environment at the heart of a community of more than 18,500 people, including over 14,000 students and 4,000 researchers from more than 120 different countries.

Postdoctoral position in multiplexed single-cell RNAseq-based antibody discovery

Mission

The lab of biomedical microfluidics (LBMM; www.epfl.ch/labs/lbmm ) at the Swiss Federal Institute of Technology in Lausanne (EPFL), is looking for postdocs with a primary training in single-cell biology, next generation sequencing or antibody discovery. Experience in droplet-microfluidics, including use of commercial devices such as the 10X Genomics platform is beneficial, but not absolutely crucial. LBMM has already developed powerful technology platforms in the field of biomedicine and genomics over the past years, also leading to the establishment of two startup companies ( www.veraxa.com and www.thera-me.ch ).

Having a comprehensive microfluidic toolbox at hand (and expanding it continuously), we are now inviting applications for highly multiplexed single-cell RNAseq-based antibody discovery. The idea behind this project is to exploit transcriptomic data sets for the discovery of new therapeutic antibodies.

The lab has a very translational focus, running a tech development lab on the EPFL campus and a translational lab in the AGORA cancer research center (with direct access to patient material). Furthermore, a strong network of clinical partners in Europe and the US has been established.

The successful candidate will closely interact with experts in microfluidics and bioinformatics, while spearheading the above project.

Main duties and responsibilities include

• Optimization of single-cell sequencing workflows
• FACS sorting of cells
• Preparation of NGS libraries
• Data analysis
• Experimental design of the screens together with bioinformaticians

Your profile

• Hands on experience in single-cell analysis and/or antibody discovery
• Good knowledge of single-cell RNAseq workflows such as 10X genomics or DropSeq, experienced in flow cytometry
• Good knowledge in cell biology and biochemistry
• Prior experience in microfluidics is advantageous but not absolutely required
• Highly motivated and keen to work in an interdisciplinary environment

We offer

• A multidisciplinary environment
• Exposure to the latest cutting-edge experimental procedures (e.g. custom single-cell microfluidic platforms)
• Preparation for a future career in academia, industry or our own startup company

Informations

For any further information, please contact: Prof. Christoph Merten, [e-mail]