Postdoc: Discovering Cable Bacteria Biosynthetic Enzymes

KU Leuven; Vlaams-Brabant; Sur place; EUR 52 000 - 66 000; Plein temps

Avantages offerts par ce poste

Fully-funded postdoctoral position

International and multidisciplinary R&

Training opportunities and access to设施

Résumé du poste

The lab of Prof. Joleen Masschelein at KU Leuven and the VIB-KU Leuven Center of Microbiology invites applications for a Postdoctoral fellow to study the biosynthesis of conductive cable bacterium structures.

The project combines chemistry and biology, including enzymology, genomics, mass spectrometry and structural biology in a highly international environment. You will work on identifying biosynthetic enzymes, producing recombinant proteins in E.

Qualifications

• PhD in microbiology, biochemistry, molecular biology, chemical biology or a closely related discipline
• First-author publications in international journals
• Strong background in microbial biochemistry, enzymology or microbial chemical biology
• Hands-on experience with recombinant protein production in E. coli and biochemical characterization
• Experience with designing, performing and interpreting in vitro enzyme assays
• Experience with mass spectrometry-based protein or metabolite analysis (LC-MS, intact protein MS, proteomics, metabolite profiling)
• Experience with molecular cloning and site-directed mutagenesis
• Excellent written and oral communication skills in English
• Strong organizational skills and ability to work independently and in a team
• Experience with multidisciplinary collaborations is an asset

Responsabilités

• Investigate the biosynthetic machinery for conductive structures in cable bacteria
• Identify and characterize candidate biosynthetic enzymes using molecular biology, recombinant protein production and purification
• Reconstruct biosynthetic pathways and uncover mechanisms of assembly of conductive molecules

Connaissances

Enzymology

Recombinant protein production

Mass spectrometry

Molecular cloning

Site-directed mutagenesis

In vitro enzyme assays

LC-MS

Team collaboration

English communication

Formation

PhD in microbiology/biochemistry

Outils

E. coli expression

LC-MS workflows

Proteomics

Description du poste

The lab of Prof. Joleen Masschelein at KU Leuven and the VIB-KU Leuven Center of Microbiology invites applications for a Postdoctoral fellow to study the biosynthesis of conductive cable bacterium structures.

The project combines chemistry and biology, including enzymology, genomics, mass spectrometry and structural biology in a highly international environment. You will work on identifying biosynthetic enzymes, producing recombinant proteins in E.