Staff Scientist

University of Rochester; United States; On-site; USD 61,000 - 85,000; Full time

Job summary

The University of Rochester in Rochester, NY seeks a Staff Scientist in the Department of Environmental Medicine and Public Health Sciences. This full-time role focuses on environmental tobacco/e-cigarette–mediated oxidative stress and inflammation using in vitro and in vivo models.

You will lead hypothesis-driven research on inflammation, circadian rhythms, and epigenetics of DNA damage/repair, and supervise lab staff, with responsibility for animal work, record keeping, and compliance with

Qualifications

• Experience leading hypothesis-driven research on inflammation and epigenetics.
• Ability to supervise and train undergraduate students, graduate students and postdoctoral fellows.

Responsibilities

• Serve as lab manager for the Principal Investigator and supervise lab activities.
• Perform and troubleshoot molecular biology assays including Western blotting, RT-PCR and ChIP.
• Oversee animal work, breeding, genotyping, and compliance with UCAR/DCM guidelines.
• Coordinate with UCAR for inspections and maintain detailed records of experiments.
• Train and evaluate staff and trainees in lab methods and safety.

Skills

Lab management

Independent researcher

Supervision of staff

Communication

Tools

Western blotting

immunostaining

ELISA

RNA isolation

DNA isolation

ChIP assay

EMSA

RT-PCR

in situ hybridization

immunohistochemistry

tissue staining

Seahorse

Luminex

cytation5

imaging

flow cytometry

spectrophotometers

Job description

As a community, the University of Rochester is defined by a deep commitment to Meliora - Ever Better. Embedded in that ideal are the values we share: equity, leadership, integrity, openness, respect, and accountability. Together, we will set the highest standards for how we treat each other to ensure our community is welcoming to all and is a place where all can thrive.

Job Location (Full Address):

601 Elmwood Ave, Rochester, New York, United States of America, 14642

Opening:

• Worker Subtype: Regular
• Time Type: Full time
• Scheduled Weekly Hours: 40
• Department: 400039 Environmental Medicine
• Work Shift: UR - Day (United States of America)
• Range: UR URG 110
• Compensation Range: $61,000.00 - $85,400.00

The referenced pay range represents the minimum and maximum compensation for this job. Individual annual salaries/hourly rates will be set within the job's compensation range, and will be determined by considering factors including, but not limited to, market data, education, experience, qualifications, expertise of the individual, and internal equity considerations.

Responsibilities:

The Department of Environmental Medicine and Public Health Sciences has an opening for a Staff Scientist. We are particularly interested in a candidate with strong background and experience in environmental tobacco/e-cigarette-mediated oxidative stress and inflammation using in vitro and in vivo models. We seek a person who will be able to lead hypothesis-driven research on inflammation, circadian rhythms, epigenetics of DNA damage/repair. Exosomes, mitochondrial dysfunction and cellular senescence using cutting edge molecular biology and biochemical approaches to investigate the impact of e-cigarette/tobacco smoke in chronic lung diseases including chronic obstructive pulmonary disease and pulmonary fibrosis.

ESSENTIAL FUNCTIONS

Serves as lab manager for a Principal Investigator. Works independently with only occasional suggestions to modify, vary or adapt standard laboratory procedures to perform research related activities to meet the specialized needs of the research projects of the Principal Investigator. Responsibilities will include: Techniques to be performed and troubleshooting: Western blotting, immunostaining, lung functioning experiments, ELISA, bone marrow and stem cell isolation, mitochondrial dysfunction by Seahorse, Northern blotting, ribonuclease protection assays, tissue section staining, various nuclear and cytoplasmic staining assays, immunohistochemistry, in situ hybridization. RNA isolation and DNA isolation, ChIP assay, EMSA (gel shift assay), RT-PCR, restriction enzyme digests, plasmid purification and extraction, quantifying results, analysis and record keeping. Perform new experiments and assays in cells and tissues. Assists and trains undergraduate students, graduate students and postdoctoral fellows to learn and perform assays, routine techniques/methods, and procedures for their experiments. Independently supervises and oversee large colonies of rodent breeding, genotyping and maintenance. Duties will include oversight of preparation of experimental substance (e.g. drugs, environmental chemicals) administration. Oversees the ordering, breeding, and euthanasia of rodents for laboratory use; ensures detailed record keeping of such activities including UCAR and DCM forms/reports is maintained and up-to-date. All animal work will be performed in compliance with DCM and UCAR guidelines. Mouse colony management and exposures to rodents are required. The Staff Scientist will communicate with DCM staff in order to ensure the health of experimental research subjects. They will also coordinate with UCAR for annual and ad-hoc inspections to ensure compliance with all UCAR polices. Provides guidance and trains and supervises other laboratory members (e.g. students, postodocs, technicians, etc.) to complete these duties. Participates and contributes to regularly-scheduled laboratory meetings. Provides a verbal account of progress on lab-specific goals, including experimental progress on research activities, as well as reporting of lab members' compliance with Federal, State, and University regulations for use of laboratory animals, hazardous materials, and general laboratory health and safety as needed. Develops, installs, and maintains operating procedures for the laboratory, and independently operates complex equipment (e.g. Luminex, cytation5, imaging, flow cytometry, seahorse, seahorse, lumicycle, fluorescent microscopes, spectrophotometers). Develops and modifies test methods and selects procedures for data collection and handling. Optimizes methods for data analysis and statistical treatment of data by establishing and updating protocols for data conversion. Interviews, trains, supervises and evaluates laboratory staff, graduate students, post-doctoral trainees and other laboratory members. Directs initial training as well as monitors and fosters continuous skill development of staff/trainees in laboratory t